FORMULATION OF GEL FROM GYNURA SEGETUM EXTRACT AND ITS ACTIVITY ON BURN WOUND HEALING
Keywords:Gynura segetum, Gel, Burn wound healing, Carbomer
Objective: The aims of this study were to formulate gel from Gynura segetum (GS) extract and evaluate its burn-healing activity.
Methods: GS extract was formulated using carbomer and carboxymethylcellulose (CMC) as a stabilizer with various concentrations. Furthermore, the gel of GS extract was evaluated, including organoleptic, pH and viscosity. A burn-healing evaluation was conducted by making a wound with a hot plate on male Wistar rats, and 600 mg of the gel was applied. Subsequently, the presence of erythema and width contraction of the burns were observed for 15 d.
Results: The result showed that the formulation containing 1 % of carbomer and 1.2 % of GS extract has the best physical stability. The gel also increased the rate of the healing process with decreased burn wound contraction (5.67 mm after 15 d) and the erythema than the control (8.50 mm after 15 d). The significance value was less than 0.05, indicating that the rate of the healing process was significantly different between the GS extract gel and the control.
Conclusion: This finding demonstrated that the gel of GS extract can significantly improve the burns wound healing process and may also be safely used for topical preparation.
Pirbalouti AG, Yousefi M, Nazari H, Karimi I, Koohpayeh A. Evaluation of burn healing properties of Arnebia euchroma and Malva sylvestris. Electron J Biol 2009;5:62-6.
Mokaddas E, Rotimi VO, Sanyal SC. In vitro activity of piperacillin/tazobactam versus other broad antibiotics against nosocomial gram-negative pathogens isolated from burn patients. J Chemother 1998;10:208-14.
Edgar DW, Fish JS, Gomez M, Wood FM. Local and systemic treatments for acute edema after burn injury: a systematic review of the literature. J Burn Care Res 2011;32:334-47.
Sommer K, Sander AL, Albig M, Weber R, Henrich D, Frank J, et al. Delayed wound repair in sepsis is associated with reduced local pro-inflammatory cytokine expression. PloS One 2013;8:e73992.
Purna SK, Babu M. Traditional medicine and practices in burn care: need for newer scientific perspectives. Burns 1998;24:387-8.
Nayak BS, Pereira LM. Catharanthus roseus flower extract has wound-healing activity in sprague dawley rats. BMC Complement Altern Med 2006;6:1-6.
Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns 2007;33:139-48.
Seow LJ, Beh HK, Sadikun A, Asmawi MZ. Preliminary phytochemical and physicochemical characterization of Gynura segetum (Lour) merr. (Compositae) leaf. Trop J Pharm Res 2013;12:777-82.
Yuan SQ, Gu GM, Wei TT. Studies on the alkaloids of Gynura segetum (Lour.) Merr. Acta Pharm Sin 1990;25:191-7.
Budiman A, Rusnawan DW, Yuliana A. Antibacterial activity of Piper betle L. extract in cream dosage forms against Staphylococcus aureus and Propionibacterium acne. J Pharm Sci Res 2018;10:493-6.
Islam MT, Rodriguez Hornedo N, Ciotti S, Ackermann C. Rheological characterization of topical carbomer gels neutralized to different pH. Pharm Res 2004;21:1192-9.
Budiman A, Praditasari A, Rahayu D, Aulifa DL. Formulation of antioxidant gel from black mulberry fruit extract (Morus nigra L.). J Pharm Bioallied Sci 2019;11:216-22.
Djerrou J, Maameri Z, Hamdo Pacha Y, Serakta M, Riachi F, Djaalab H, et al. Effect of virgin fatty oil of Pistacia lentiscus on experimental burn wound’s healing in rabbits. Afr J Tradit Complement Altern Med 2010;7:258-63.
Shivhare Y, Singour PK, Patil UK, Pawar RS. Wound healing potential of methanolic extract of Trichosanthes dioica Roxb (fruits) in rats. J Ethnopharmacol 2010;127:614-9.
Arun M, Satish S, Anima P. Herbal boon for wounds. Int J Pharm Pharm Sci 2013;5:1-2.
Tsala DE, Amadou D, Habtemariam S. Natural wound healing and bioactive natural products. Phytopharmacology 2013;4:532-60.
Liu P, Zhai M, Li J, Peng J, Wu J. Radiation preparation and swelling behavior of sodium carboxymethyl cellulose hydrogels. Radiat Phys Chem 2002;63:525-8.
Islam MT, Rodríguez Hornedo N, Ciotti S, Ackermann C. Fourier transform infrared spectroscopy for the analysis of neutralizer-carbomer and surfactant-carbomer interactions in aqueous, hydroalcoholic, and anhydrous gel formulations. AAPS J 2004;6:61-7.
Lane ME. Skin penetration enhancers. Int J Pharm 2013;447:12-21.
Patil PM, Wankhede SB, Chaudhari PD. Stability-indicating HPTLC method for simultaneous determination of ketoprofen, methyl paraben and propyl paraben in gel formulation. J Pharm Res 2013;6:945-53.
Dotto SR, Travassos RMC, De Oliveira EPM, de Lima Machado ME, Martins JL. Evaluation of ethylenediaminetetraacetic acid (EDTA) solution and gel for smear layer removal. Aust Endod J 2007;33:62-5.
Maia AM, Baby AR, Pinto CA, Yasaka WJ, Suenaga E, Kaneko TM, et al. Influence of sodium metabisulfite and glutathione on the stability of vitamin C in O/W emulsion and extemporaneous aqueous gel. Int J Pharm 2006;322:130-5.
Jyothi D, Koland M. Formulation and evaluation of an herbal anti-inflammatory gel containing Trigonella foenum greacum seed extract. Int J Pharm Pharm Sci 2016;8:41-4.
Pednekar A, Dandagi P, Gadad A, Mastiholimath V. Formulation and characterization of meloxicam loaded emulgel for topical application. Int J Pharm Pharm Sci 2015;7:216-22.
Machekposhtia SA, Soltani M, Raahemifarc K. PASI area and erythema scoring using YIQ color space. Dermatol Res Skin Care 2017;1:8-14.
Budiman A, Khoerunnisa R, Qoriah TA. Wound-healing test of Piper betle leaf extract and aloe vera in gel preparation. Int J Appl Pharm 2018;10:86-91.