Cellular Uptake Study and Cytotoxicity Study of Resveratrol-Gold-PEG-Folate (RSV-Au-PEG-FA) Nanoparticles on HeLa Human Cervical Cancer Cell Line
Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in RSV targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line.
Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.
Results: Cellular uptake study has shown that RSV-Au-PEG-FA is potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA IC5067.06±2.14mM and RSV IC509.66±1.44 mM on HeLa cells
Conclusion: These results indicated that RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively. However, RSV-Au-PEG-FA nanoparticles need higher concentration to cause toxic effect on HeLa cells compared to free RSV.
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