CELLULAR UPTAKE STUDY AND CYTOTOXICITY STUDY OF RESVERATROL-GOLD-PEG-FOLATE (RSV-AU-PEG-FA) NANOPARTICLES ON HELA HUMAN CERVICAL CANCER CELL LINE

  • DARA A. PUTRI Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia
  • SUTRIYO SUTRIYO Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia
  • FADLINA C. SAPUTRI Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia

Abstract

Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line.


Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.


Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 mM and RSV IC50 9.66±1.44 mM on HeLa cells


Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

Keywords: Resveratrol, Gold nanoparticles, Folic acid, Active targeting, Cellular uptake, Cytotoxicity, HeLa cells

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PUTRI, D. A., SUTRIYO, S., & SAPUTRI, F. C. (2020). CELLULAR UPTAKE STUDY AND CYTOTOXICITY STUDY OF RESVERATROL-GOLD-PEG-FOLATE (RSV-AU-PEG-FA) NANOPARTICLES ON HELA HUMAN CERVICAL CANCER CELL LINE. International Journal of Applied Pharmaceutics, 12(4), 113-118. https://doi.org/10.22159/ijap.2020v12i4.37307
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