SYNTHESIS AND STABILITY TEST OF RESVERATROL-CONJUGATED GOLD NANOPARTICLE WITH POLYVINYL ALCOHOL STABILIZATION

  • SUTRIYO SUTRIYO Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • RADITYA ISWANDANA Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • MARINA DWI HAFSHARI Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia

Abstract

Objective: Gold nanoparticles (AuNPs) have been developed as a promising effective site-specific drug to increase drug efficacy and reduce potential
side effects. However, AuNPs are unstable because they easily aggregate. This study aims to produce stable resveratrol (RSV)-conjugated AuNPs using
polyvinyl alcohol (PVA).
Methods: AuNPs were synthesized using the Turkevich method, which involves the reduction of chloroauric acid with sodium citrate as a reductor.
AuNPs were then modified with PVA as a stabilizing agent and conjugated with RSV as a drug model in the carrier system. The formed conjugates were
characterized using ultraviolet–visible spectrophotometry, Fourier transform infrared spectroscopy, particle size analysis, and high-performance
liquid chromatography. Furthermore, stability tests were performed in various media (2% bovine serum albumin [BSA], 1% cysteine, phosphatebuffered
saline [PBS] pH 4, PBS pH 7.4, and 0.9% NaCl) for 28 days.
Results: RSV–AuNPs–PVA had a particle size of 78.75 nm, with polydispersity index (PDI) of 0.356, zeta potential of −36.1 mV, and highest entrapment
efficiency of 78.1%±0.7. RSV–AuNPs without PVA stabilization had a particle size of 51.97 nm, with PDI of 0.694 and zeta potential of −24.6 mV. The
results of the stability tests demonstrated that RSV–AuNPs–PVA was stable in 2% BSA, PBS pH 7.4, PBS pH 4, and NaCl 0.9% and were unstable in 1%
cysteine. RSV–AuNPs without PVA were stable in 2% BSA and PBS pH 7.4 and unstable in 1% cysteine, PBS pH 4, and 0.9% NaCl.
Conclusion: PVA can improve the physical stability of RSV-AuNPs conjugates.

Keywords: Conjugate, Gold nanoparticles, Polyvinyl alcohol, Resveratrol, Stability

References

1. Kumar CG, Poornachandra Y, Mamidyala SK. Green synthesis of
bacterial gold nanoparticles conjugated to resveratrol as delivery
vehicles. Colloids Surf B Biointerfaces 2014;123:311-7.
2. Kadian R. Nanoparticles: A promising drug delivery approach. Asian J
Pharm Clin Res 2018;11:30-5.
3. Lavanya N, Muzib I, Jithan A, Umamahesh B. Novel nanoparticles for
the oral delivery of low molecular weight heparin: In vitro and in vivo
assessment. Asian J Pharm Clin Res 2017;10:254-61.
4. Kong FY, Zhang JW, Li RF, Wang ZX, Wang WJ, Wang W. Unique
roles of gold nanoparticles in drug delivery, targeting and imaging
applications. Molecules 2017;22:E1445.
5. Ajnai G, Chiu A, Kan T, Cheng C, Tsai T, Chang J. Trends of gold
nanoparticle-based drug delivery system in cancer therapy. J Exp Clin
Med 2014;6:172-8.
6. Sharma N, Bhatt G, Kothiyal P. Gold nanoparticles synthesis,
properties, and forthcoming applications-a review. Indian J Pharm Biol
Res 2015;3:13-27.
7. Kumar A, Mansour H, Friedman A, Blough E. Nanomedicine in Drug
Delivery. Boca Raton: CRC Press; 2017.
8. Rahme K, Chen L, Hobbs RG, Morris MA, O’Driscoll C, Holmes JD.
PEGylated gold nanoparticles: Polymer quantification as a function of
PEG lengths and nanoparticle dimensions. RSC Adv 2013;3:6085-94.
9. Pac?awski K, Streszewski B, Jaworski W, Luty-B?ocho M, Fitzner K.
Gold nanoparticles formation via gold (III) chloride complex ions
reduction with glucose in the batch and in the flow microreactor
systems. Colloids Surf Physicochem Eng Asp 2012;413:208-15.
10. Gaikwad P, Sharma S, Sudarshan K, Kumar V, Kshirsagar A, Pujari P.
Molecular packing of polyvinyl alcohol in PVA-gold nanoparticles
composites and its role on thermo-mechanical properties. Polym
Compos 2016;39:1-6.
11. Park SY, Chae SY, Park JO, Lee KJ, Park G. Gold-conjugated
resveratrol nanoparticles attenuate the invasion and MMP-9 and COX-
2 expression in breast cancer cells. Oncol Rep 2016;35:3248-56.
12. Souto AA, Carneiro MC, Seferin M, Senna MJ, Conz A, Gobbi K.
Determination of trans-resveratrol concentrations in Brazilian red
wines by HPLC. J Food Compost Anal 2001;14:441-5.
13. Djajadisastra J, Sutriyo S, Purnamasari P, Pujiyanto A. Antioxidant
activity of gold nanoparticles using gum Arabic as a stabilizing agent.
Int J Pharm Pharm Sci 2014;6:462-5.
14. Zhang Y, Chu W, Foroushani AD, Wang H, Li D, Liu J, et al. New
gold nanostructures for sensor applications: A review. Materials (Basel)
2014;7:5169-201.
15. Esumi K, Suzuki A, Yamahira A, Torigoe K. Role of poly (amidoamine)
dendrimers for preparing nanoparticles of gold, platinum, and silver.
Langmuir 2000;16:2604-8.
16. Pertiwi RD, Djajadisastra J, Mutalib A, Pujiyanto A. Preparation of
gold nanoparticles with based on conjugated gum Arabic vincristine
and evaluation of their in vitro characteristics. J Ilmu Kefarmasian
Indones 2018;16:6-11.
17. Avadi MR, Sadeghi AM, Mohammadpour N, Abedin S, Atyabi F,
Dinarvand R, et al. Preparation and characterization of insulin
nanoparticles using chitosan and Arabic gum with ionic gelation
method. Nanomedicine 2010;6:58-63.
18. Mohanraj VJ, Chen Y. Nanoparticles a review. Trop J Pharm Res
2006;5:561-73.
19. Chen W. What is Zeta Potential? San Diego: The American Filtration
and Separations Society; 2016.
20. Kattumuri V. Gold nanoparticles for biomedical applications: Synthesis,
characterization, in vitro and in vivo studies. USA: Dissertation the
Faculty of Graduate School, University of Missouri, Columbia; 2006.
21. Oliveira J, Prado A, Keijok W, Ribeiro M, Pontes M, Nogueira B,
et al. A helpful method for controlled synthesis of monodisperse
gold nanoparticles through response surface modeling. Arab J Chem
2017;1-11.
22. Vaseghi A, Safaie N, Bakhshinejad B, Mohsenifar A, Sadeghizadeh M.
Detection of Pseudomonas syringae pathovars by thiol-linked DNAgold
nanoparticle probes. Sens Actuators B Chem 2013;181:644-51.
23. Wagers K, Chui T, Adem S. Effect of pH on the stability of gold
nanoparticles and their application for melamine detection in infant
formula. IOSR J Appl Chem 2014;7:15-20.
24. Butt H, Graf K, Kappl M. Physics and Chemistry of Interfaces.
Weinheim: Wiley-VCH; 2006.
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SUTRIYO, S., ISWANDANA, R., & HAFSHARI, M. D. (2020). SYNTHESIS AND STABILITY TEST OF RESVERATROL-CONJUGATED GOLD NANOPARTICLE WITH POLYVINYL ALCOHOL STABILIZATION. International Journal of Applied Pharmaceutics, 12(1), 221-229. https://doi.org/10.22159/ijap.2020.v12s1.FF049
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