THERAPEUTIC EVALUATION OF CHEMICALLY SYNTHESIZED COPPER NANOPARTICLES TO PROMOTE FULL-THICKNESS EXCISIONAL WOUND HEALING

  • ASHISH KUMAR Department of Pharmaceutics, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh 201303, India
  • VINAY PANDIT Department Pharmaceutics, Laureate Institute of Pharmacy, Himachal Pradesh 177101, India
  • UPENDRA NAGAICH Department of Pharmaceutics, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh 201303, India

Abstract

Objective: The purpose of this research was, synthesis of copper nanoparticles using environment friendly cementation method and evaluate their wound healing property on full-thickness excisional wound.


Methods: Present study reports the synthesis of CNPs by single-step cementation method. Evaluation of CNPs was endorsed by morphological and chemical properties. Furthermore, CNPs was evaluated for its antibacterial potential and invitro hemocompatibility. Additionally, pharmacological evaluation of CNPs was assessed against excisional wound.


Results: Characterization of final product indicate, particle size of CNPs were ranging from 100-150 nm. CNPs showed significant antibacterial activity (A= 2.1±0.1 mm, B =2.1±0.1 mm, C = 1.9±0.2 mm, at 10µg/ml), along with superior hemocompatibility (RBC cell survival 97±1 %). Further CNPs formulation shows increased level of anti-inflammatory cytokinin’s (IL-10, 42.7%) as compared to standard (STD), vehicle control, and normal control groups, attributed to accelerated wound healing (p<0.05 vs STD).


Conclusion: The consequences the present investigation endorse the accelerated wound healing potential of CNPs with its anti-inflammatory potential.

Keywords: Copper nanoparticle, Biocompatibility, Antibacterial potential, Wound healing

References

1. Godbout JP, Glaser R. Stress-induced immune dysregulation: Implications for wound healing, infectious disease and cancer. J Neuroimmune Pharm 2006;1:421–7.
2. Mühl H. Pro-inflammatory signaling by IL-10 and IL-22: bad habit stirred up by interferons? Front Immunol 2013;4:18.
3. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2018;9:7204–18.
4. Larouche J, Sheoran S, Maruyama K, Martino MM. Immune regulation of skin wound healing: mechanisms and novel therapeutic targets. Adv Wound Care 2018;7:209–31.
5. Guo S, DiPietro LA. Critical review in oral biology and medicine: factors affecting wound healing. J Dent Res 2010;89:219–29.
6. Borkow G. Using copper to improve the well-being of the skin. Curr Chem Biol 2015;8:89–102.
7. Vincent M, Hartemann P, Engels Deutsch M. Antimicrobial applications of copper. Int J Hyg Environ Health 2016;219:585–91.
8. Mahavir J, Sneh L, Preeti K, Tulika M. Application of nanostructures in antimicrobial therapy. Int J Appl Pharm 2018;10:11–25.
9. Gopal A, Kant V, Gopalakrishnan A, Tandan SK, Kumar D. Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats. Eur J Pharmacol 2014;731:8–19.
10. Levy SB, Bonnie M. Antibacterial resistance worldwide: causes, challenges and responses. Nature Med 2004;1:122–9.
11. Manyasree D, Peddi KM, Ravikumar R. CuO nanoparticles: synthesis, characterization and their bactericidal efficacy. Int J Appl Pharm 2017;9:71–4.
12. Bahadar H, Maqbool F, Niaz K, Abdollahi M. Toxicity of nanoparticles and an overview of current experimental models. Iran Biomed J 2016;20:1–11.
13. Sharma AK, Kumar A, Taneja G, Nagaich U, Deep A, Rajput SK. Synthesis and preliminary therapeutic evaluation of copper nanoparticles against diabetes mellitus and-induced micro-(renal) and macro-vascular (vascular endothelial and cardiovascular) abnormalities in rats. RSC Adv 2016;6:36870–80.
14. Saber Tehrani M, Rastegar F, Parchehbaf A, Rezvani Z. Determination of copper by flame atomic absorption spectrometry after preconcentration with activated carbon impregnated with a new Schiff base. Chinese J Chem 2005;23:1437–42.
15. Chandra S, Kumar A, Tomar PK. Synthesis and characterization of copper nanoparticles by reducing agents. J Saudi Chem Soc 2014;1:149–53.
16. Lim J, Yeap SP, Che HX, Low SC. Characterization of the magnetic nanoparticle by dynamic light scattering. Nanoscale Res Lett 2013;8:381.
17. Gittings M, Saville D. The determination of hydrodynamic size and zeta potential from electrophoretic mobility and light scattering measurements. Colloids Surfaces A Physicochem Eng Asp 1998;141:111–7.
18. Rodriguez P, Plana D, Fermin DJ, Koper MTM. New insights into the catalytic activity of gold nanoparticles for CO oxidation in electrochemical media. J Catal 2014;311:182–9.
19. Mühlfeld C, Rothen Rutishauser B, Vanhecke D, Blank F, Gehr P, Ochs M. Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopy. Part Fibre Toxicol 2007;4:11.
20. Rades S, Hodoroaba VD, Salge T, Wirth T, Lobera MP, Labrador RH, et al. High-resolution imaging with SEM/T-SEM, EDX and SAM as a combined methodical approach for morphological and elemental analyses of single engineered nanoparticles. RSC Adv 2014;4:49577–87.
21. Kumar V, Sharma AK, Rajput SK, Pal M, Dhiman N. Pharmacognostic and pharmacological evaluation of Eulaliopsis binata plant extracts by measuring in vitro/in vivo safety profile and anti-microbial potential. Toxicol Res 2018;7:454–64.
22. Shakeel A, Singh A, Das S, Suhag D, Sharma AK, Rajput SK, et al. Synthesis and morphological insight of new biocompatible smart hydrogels. J Polym Res 2017;24:113.
23. Sangam S, Gupta A, Shakeel A, Bhattacharya R, Sharma AK, Suhag D, et al. Sustainable synthesis of single-crystalline sulphur-doped graphene quantum dots for bioimaging and beyond. Green Chem 2018;20:4245–59.
24. Mendes JJ, Leandro CI, Bonaparte DP, Pinto AL. A rat model of diabetic wound infection for the evaluation of topical antimicrobial therapies. Comp Med 2012;62:37–48.
25. Gal P, Kilik R, Mokry M, Vidinsky B, Vasilenko T, Mozes S, et al. Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: Standardization of semi-quantitative and quantitative histological assessments. Vet Med 2008;53:652–9.
26. Kaur P, Sharma AK, Nag D, Das A, Datta S, Ganguli A, et al. Novel nano-insulin formulation modulates cytokine secretion and remodeling to accelerate diabetic wound healing. Nanomed Nanotechnol 2019;15:47–57.
27. Joseph E, Singhvi G. Multifunctional nanocrystals for cancer therapy: a potential nanocarrier. Nanomaterials Drug Delivery Ther 2019;1:91–116.
28. Suhag D, Sharma AK, Patni P, Garg SK, Rajput SK, Chakrabarti S, et al. Hydrothermally functionalized biocompatible nitrogen-doped graphene nanosheet based biomimetic platforms for nitric oxide detection. J Mater Chem B 2016;4:4780–9.
29. Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: a comprehensive review. Adv Wound Care 2014;3:445–64.
30. Efron PA, Moldawer LL. Cytokines and wound healing: the role of cytokine and anticytokine therapy in the repair response. J Burn Care Rehabil 2004;25:149–60.
31. Hussain A, AlAjmi MF, Rehman MT, Amir S, Husain FM, Alsalme A, et al. Copper(II) complexes as potential anticancer and nonsteroidal anti-inflammatory agents: in vitro and in vivo studies. Sci Rep 2019;9:5237.
32. Bettinger DA, Pellicane JV, Tarry WC, Yager DR, Diegelmann RF, Lee R, et al. The role of inflammatory cytokines in wound healing: accelerated healing in endotoxin-resistant mice. J Trauma 1994;36:810–3.
33. Strbo N, Yin N, Stojadinovic O. Innate and adaptive immune responses in wound epithelialization. Adv Wound Care 2014;3:492–501.
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KUMAR, A., PANDIT, V., & NAGAICH, U. (2020). THERAPEUTIC EVALUATION OF CHEMICALLY SYNTHESIZED COPPER NANOPARTICLES TO PROMOTE FULL-THICKNESS EXCISIONAL WOUND HEALING. International Journal of Applied Pharmaceutics, 12(6), 136-142. https://doi.org/10.22159/ijap.2020v12i6.38869
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