• ASHISH KUMAR Department of Pharmaceutics, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh 201303, India, Amity Institute of Pharmacy, Amity University, Haryana 122413, India
  • VINAY PANDIT Department Pharmaceutics, Laureate Institute of Pharmacy, Himachal Pradesh 177101, India
  • UPENDRA NAGAICH Department of Pharmaceutics, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh 201303, India


Objective: The present study focuses on the development and optimization of copper nanoparticles (CNPs) loaded hydrogel for the treatment of dermal burn injuries.

Methods: CNPs gel was prepared by dispersing the variable concentration of polyvinylpyrrolidone (PVP K30) and hydroxypropyl methylcellulose (HPMC) in distilled water, PEG 400, and copper nanoparticles. factor screening study was performed for identification of influential factors, followed by optimization study using three-factor Box-Behnken design.

Results: Optimized nanogel formulation, when compared to normal control (NC), shows a significant reduction of pro-inflammatory cytokines (IL-6 = 39.74 % and TNF-α =49.37%) and increased level of anti-inflammatory cytokines (IL-10 = 30.90%), indicating reduced inflammation. Further, the wound closure rate of CNPs gel shows significant (12.27 %) wound closure as compared to the NC group and complete wound closure (100 %) on the 14th day, indicating accelerated wound healing.

Conclusion: the present investigation endorses accelerated scar-free, accelerated wound healing potential of copper nanoparticles gel with anti-inflammatory potential.

Keywords: Copper nanoparticles, Optimization, Hydrogel, Wound healing


1. Menna C, Calista N, Aurino L, Dwijayanti A. Aloe vera vs. Silver sulfadiazine for treating second-degree burn wounds: evidence-based case report. Int J Appl Pharm 2019;11:146–8.
2. Evers LH, Bhavsar D, Mailander P. The biology of burn injury. Exp Dermatol 2010;19:777–83.
3. Singh A. Hydrogel nanotubes with ice helices as exotic nanostructures for diabetic wound healing. Mater Horizons 2019;6:274–84.
4. Rose LF, Chan RK. The burn wound microenvironment. Adv Wound Care 2016;5:106–18.
5. Ferreira SLC. Box-behnken design: an alternative for the optimization of analytical methods. Anal Chim Acta 2007;2:179–86.
6. Taneja G, Sud A, Pendse N, Panigrahi A, Kumar A, Sharma AK. Nano-medicine and vascular endothelial dysfunction: options and delivery strategies. Cardiovasc Toxicol 2019;19:1–12.
7. Nochos A, Douroumis D, Bouropoulos N. In vitro release of bovine serum albumin from alginate/HPMC hydrogel beads. Carbohydr Polym 2008;3:451–7.
8. Kumar A, Pandit V, Nagaich U. Therapeutic evaluation of chemically synthesized copper nanoparticles to promote full-thickness excisional wound healing. Int J Appl Pharm 2020;12:136–42.
9. Kamarudin NB, Sharma S, Gupta A, Kee CG, Chik SMSBT, Gupta R. Statistical investigation of extraction parameters of keratin from chicken feather using Design-Expert 3. Biotech 2017;7:1–9.
10. Sharma AK, Kumar A, Taneja G, Nagaich U, Deep A, Rajput SK. Synthesis and preliminary therapeutic evaluation of copper nanoparticles against diabetes mellitus and-induced micro-(renal) and macro-vascular (vascular endothelial and cardiovascular) abnormalities in rats. RSC Adv 2016;6:36870–80.
11. Kim B, Peppas NA. In vitro release behavior and stability of insulin in complexation hydrogels as oral drug delivery carriers. Int J Pharm 2003;266:29–37.
12. Valdes O. Methamidophos removal from aqueous solutions using a super adsorbent based on crosslinked poly(vinyl alcohol) hydrogel. J Appl Polym Sci 2018;5:45964.
13. Cai EZ. Creation of consistent burn wounds: a rat model. Arch Plast Surg 2014;41:317–24.
14. Hannestad J, Dellagioia N, Bloch M. The effect of antidepressant medication treatment on serum levels of inflammatory cytokines: a meta-analysis. Neroew 2011;36:2452–9.
15. Manyasree D, Peddi KM, Ravikumar R. CuO nanoparticles: synthesis, characterization and their bactericidal efficacy. Int J Appl Pharm 2017;9:71–4.
16. Sharma AK. Combined and individual strategy of exercise generated preconditioning and low dose copper nanoparticles serve as superlative approach to ameliorate ISO-induced myocardial infarction in rats. Pharmacol Reports 2018;70:789–95.
17. Sharma AK, Kumar A, Sahu M, Sharma G, Datusalia AK, Rajput SK. Exercise preconditioning and low dose copper nanoparticles exhibits cardioprotection through targeting GSK-3? phosphorylation in ischemia/reperfusion induced myocardial infarction. Microvasc Res 2018;120:59–66.
94 Views | 118 Downloads
How to Cite
KUMAR, A., PANDIT, V., & NAGAICH, U. (2021). PREPARATION AND EVALUATION OF COPPER NANOPARTICLES LOADED HYDROGEL FOR BURNS. International Journal of Applied Pharmaceutics, 13(2), 180-189.
Original Article(s)