THE VIABILITY TEST OF SAPPAN WOOD (CAESALPINIA SAPPAN L.) ETHANOL EXTRACT IN THE H9C2 CELL LINE

  • IFA SULISTIYORINI Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia
  • RATU SAFITRI Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia
  • RONNY LESMANA Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
  • MAS RIZKY A. A. SYAMSUNARNO Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, 2Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia

Abstract

Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE).


Methods: Sappan wood was extracted in 96% ethanol and divided into dose concentrations of 2.5, 5, 10, 50, 100, and 150 μg/ml, with deferiprone used as a control. Cell viability was assessed using the PrestoBlue Cell Viability Reagent, according to manufacturer protocols.


Results: Microscopic examination showed that the cell viability of H9C2 was preserved by SWEE treatments at a dose of 10 μg/ml and suggested dose concentrations of 50 μg/ml of SWEE. The percentage of viable cells was greater than 95% with a dose concentration of 10 μg/ml of SWEE, but it was significantly reduced with a dose concentration of 50 μg/ml of SWEE (p<0.05).


Conclusion: The optimal dose concentration of SWEE to reach 95% cell viability was 10 μg/ml.

Keywords: H9C2 cell line, iron overload, Sappan wood ethanol extract

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SULISTIYORINI, I., SAFITRI, R., LESMANA, R., & SYAMSUNARNO, M. R. A. A. (2020). THE VIABILITY TEST OF SAPPAN WOOD (CAESALPINIA SAPPAN L.) ETHANOL EXTRACT IN THE H9C2 CELL LINE. International Journal of Applied Pharmaceutics, 12(3). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39479
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