SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY HPTLC

Authors

  • MRUNALINI H. KULKARNI Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • POONAM R. INAMDAR Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • PALLAVI DHEKALE Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • AMRITA THAKUR Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • SWATI MUTHA Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • NEETA RAI Department of Pharmacy, School of Pharmacy Vishwakarma University, Pune
  • VISHAL GALAVE BITS PILANI, Hyderabad

Keywords:

Valsartan, Hydrochlorothiazide, HPTLC, Tablet dosage Form, ICH guidelines

Abstract

Objective: The prevalence of Hypertension in India is estimated to be greater than 45% in middle age population, Elevated hypertension is one of the known causes of CHD, strokes and death. The objective of this work was to develop a simple, accurate and robust HPTLC method   was developed for simultaneous estimation of Valsartan and Hydrochlorothiazide in bulk and tablets.

Methods: The mobile phase optimized by HPTLC method consists of Toluene: Ethyl acetate: formic acid in the ratio of (3:7:0.3, v/v/v). The solvent front was run up to distance of 80 cm; which took 15 mins for development of TLC plate. Analytical Wavelength for UV detection selected was at 225 nm.

Results: Retention factor was found to be 0.74 ± 0.02 and 0.45 ± 0.02 for VAL and HTZ respectively. Developed chromatographic method was validated as per ICH, Q2(R1) guidelines. Linearity was found at concentration range 300-1800 ng/spot (r2 = 0.999), 50-300ng/spot (r2 = 0.9991) for VAL and HTZ respectively. LOD were found to be 23.02 ng/spot and 4.12ng/spot respectively for Valsartan and Hydrochlorothiazide. LOQ was found to be 69.76ng/spot and 12.5ng/spot for, Valsartan and Hydrochlorothiazide respectively.

Conclusion: Percentage RSD was found out to less than 2 indicating the developed method was precise and can be successively applied to pharmaceutical formulation. No interferences with the excipients were reported.

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References

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Published

21-06-2022

How to Cite

KULKARNI, M. H., INAMDAR, P. R., DHEKALE, P., THAKUR, A., MUTHA, S., RAI, N., & GALAVE, V. (2022). SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY HPTLC. International Journal of Applied Pharmaceutics, 14. Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/45524

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