ISOLATION AND CHARECTERIZATION OF TAMARIND SEED POLYSACCHARIDESâ€“A NATURAL RELEASE RETARDANT

Trideep Saikia; Jonab Ali; Biswajit Das

doi:10.22159/ijcpr.2017v9i4.20972

Trideep Saikia Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017
Jonab Ali Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017
Biswajit Das Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017

DOI https://doi.org/10.22159/ijcpr.2017v9i4.20972

Abstract

Objective: The main objective was to isolate and characterise a naturally obtain polysaccharides which have the property to formulate sustain release product.

Methods: Tamarind Seed Polysaccharides (TSP) was isolated from seed of Tamarindus indica by crushed the seed into powder and boiled with water at 45 Â°C to extract the polysaccharides. After boiling for 12 h the supernatant liquids were collected and stored in cool place. After the liquids become cooled acetone was added and freeze at-40 Â°C. Freeze materials then lyophilized to extract out the Tamarind seed polysaccharides. After that polysaccharide was evaluated for organoleptic, micromeritic and other characterization parameter along with microbial contamination

Results: Yield of polysaccharides was found to be 16.85%. As per the micromeritic property flow of polysaccharides, tapped and bulk density was found in acceptable range. Microbial studies confirmed that TSP doesn't support microbial growth.

Conclusion: The polysaccharides were isolated, extracted and characterized. The characterization was carried out for different physiochemical properties and powder properties.

Keywords: Tamarind seed polysaccharides (TSP), Freeze drying, Differential Scanning Calorimetry (DSC), Acute Toxicity

References

1. Baveja SK, Rao KV, Arora J. Examination of natural gums and mucilages as sustaining materials in tablet dosage forms. Indian J Pharm Sci 1989;5:115-9.
2. Samina Kabir khanzada, W Shaikh, Shahzadi Sofia, TG Kazi, K Usmanghani, Amina Kabir, et al. Chemical constituents of Tamarindus indica medicinal plant in Sindh. Pakistan J Bot 2008;40:2553-9.
3. Yerram Chandramouli, Shaik Firoz, Amaravathi Vikram, B Mahitha, B Rubia Yasmeen, K Hemanthpavankumar. Tamarind seed polysaccharide (TSP)-An adaptable excipient for novel drug delivery systems. Int J Pharm Practice Drug Res 2012;2:57-63.
4. Phani Kumar GK, Gangarao B, Kotha NS, Lova R. Isolation and evaluation of tamarind seed polysaccharide being used as a polymer in pharmaceutical dosage forms. Res J Pharm Biol Chem Sci 2011;2:274.
5. Barath Srinivasan, Anusha Ganta, Deveswaran Rajamanikam, Basavaraj Basappa, Madhavan Varadharajan. Evaluation of tamarind seed polysaccharides as drug release retardant. Int J Pharm Sci Rev Res 2001;9:27-31.
6. Anonymous. The British Pharmacopoeia. British Pharmacopoeia Commission. 200 Her Majestyâ€™s stationary office. London. A; 2004. p. 13.
7. Kulkarni G. Development of controlled release spheroids using natural polysaccharide as release modifier. Drug Delivery 2005;12:201-6.
8. Kulkarni D, Ddwivedi DK, Sarin JPS, Singh S. Tamarind seed polyose: a potential polysaccharide for sustained release of verapamil hydrochloride as a model drug. Indian J Pharm Sci 1997;59:1-7.
9. Das B, Dash S, Choudhury RC, Chakraborty J, Deb Roy S. Optimization and characterization of purified polysaccharide from Terminalia Belarica Gum as pharmaceutical excipient. Int J Pharm Res Allied Sci 2014;3:21-9.
10. Rashmi Manchanda, Arora SC, Rajesh Manchanda. Tamarind seed polysaccharide and its modifications versatile pharmaceutical excipientsâ€“a review. Int J PharmTech Res 2014;6:412-20.
11. Anonymous. Determination of the GRAS status of the addition of tamarind seed polysaccharide to conventional foods as a stabilizer and thickener. Available from: https://www.fda.gov/ downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm403104.pdf. [Last accessed on 15 Dec 2016]