ISOLATION AND CHARECTERIZATION OF TAMARIND SEED POLYSACCHARIDESâ€“A NATURAL RELEASE RETARDANT
Objective: The main objective was to isolate and characterise a naturally obtain polysaccharides which have the property to formulate sustain release product.
Methods: Tamarind Seed Polysaccharides (TSP) was isolated from seed of Tamarindus indica by crushed the seed into powder and boiled with water at 45 Â°C to extract the polysaccharides. After boiling for 12 h the supernatant liquids were collected and stored in cool place. After the liquids become cooled acetone was added and freeze at-40 Â°C. Freeze materials then lyophilized to extract out the Tamarind seed polysaccharides. After that polysaccharide was evaluated for organoleptic, micromeritic and other characterization parameter along with microbial contamination
Results: Yield of polysaccharides was found to be 16.85%. As per the micromeritic property flow of polysaccharides, tapped and bulk density was found in acceptable range. Microbial studies confirmed that TSP doesn't support microbial growth.
Conclusion: The polysaccharides were isolated, extracted and characterized. The characterization was carried out for different physiochemical properties and powder properties.
2. Samina Kabir khanzada, W Shaikh, Shahzadi Sofia, TG Kazi, K Usmanghani, Amina Kabir, et al. Chemical constituents of Tamarindus indica medicinal plant in Sindh. Pakistan J Bot 2008;40:2553-9.
3. Yerram Chandramouli, Shaik Firoz, Amaravathi Vikram, B Mahitha, B Rubia Yasmeen, K Hemanthpavankumar. Tamarind seed polysaccharide (TSP)-An adaptable excipient for novel drug delivery systems. Int J Pharm Practice Drug Res 2012;2:57-63.
4. Phani Kumar GK, Gangarao B, Kotha NS, Lova R. Isolation and evaluation of tamarind seed polysaccharide being used as a polymer in pharmaceutical dosage forms. Res J Pharm Biol Chem Sci 2011;2:274.
5. Barath Srinivasan, Anusha Ganta, Deveswaran Rajamanikam, Basavaraj Basappa, Madhavan Varadharajan. Evaluation of tamarind seed polysaccharides as drug release retardant. Int J Pharm Sci Rev Res 2001;9:27-31.
6. Anonymous. The British Pharmacopoeia. British Pharmacopoeia Commission. 200 Her Majestyâ€™s stationary office. London. A; 2004. p. 13.
7. Kulkarni G. Development of controlled release spheroids using natural polysaccharide as release modifier. Drug Delivery 2005;12:201-6.
8. Kulkarni D, Ddwivedi DK, Sarin JPS, Singh S. Tamarind seed polyose: a potential polysaccharide for sustained release of verapamil hydrochloride as a model drug. Indian J Pharm Sci 1997;59:1-7.
9. Das B, Dash S, Choudhury RC, Chakraborty J, Deb Roy S. Optimization and characterization of purified polysaccharide from Terminalia Belarica Gum as pharmaceutical excipient. Int J Pharm Res Allied Sci 2014;3:21-9.
10. Rashmi Manchanda, Arora SC, Rajesh Manchanda. Tamarind seed polysaccharide and its modifications versatile pharmaceutical excipientsâ€“a review. Int J PharmTech Res 2014;6:412-20.
11. Anonymous. Determination of the GRAS status of the addition of tamarind seed polysaccharide to conventional foods as a stabilizer and thickener. Available from: https://www.fda.gov/ downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm403104.pdf. [Last accessed on 15 Dec 2016]