PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF DELONIX REGIA (HOOK) RAF. BARK (FAMILY-LEGUMINOSAE)
Keywords:Delonix regia, Anti-microbial, Agar disc diffusion method, Zone of Inhibition
Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.
Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.
Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.
Hussain M, Shahid MR, Khalid HJ, Khan MRU, Aziz A, Majeed A. In vitro comparative study of antimicrobial activity of whole plant and rootâ€™s bark of Delonix regia (Bojer Ex. Hook). Int J Pharma Sci 2014;4:736-41.
Vivek MN, Swamy SHC, Manasa M, Kambar Y, Chaithra M, Mallikarjun N, et al. Antioxidant activity of leaf and flower extract of Caesalpinia pulcherrima, Delonix regia and Peltaphorum ferrugineum. JAPS 2013;3:64-71.
Patil DD, Mhaske DK, Machindra AP, Wadhawa GC. Antibacterial and antioxidant, the anti-inflammatory study of leaves and root Delonix regia. Int J Pharm Chem Sci 2014;3:131-5.
Nagarajan SS, Muthusamy P, Ramalingam R. An overview-royal poinciana. J Pharmacogn Phytochem 2015;7:232-8.
Kurian S, Josekumar VS. Phytochemical screening, antimicrobial activity and brine shrimp lethality bioassay of different extracts of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ. (family: fabaceae). Int J Pharm Sci 2017;9:1-6.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci 2011;1:98-106.
Ibironke AA, Sarah ON, Adewale A. Anti-microbial activity and phytochemical screening of five selected seeds from Nigeria. Int J Biomed Pharm Sci 2010;4:104-6.
Chetan PS, Murugananthan G, Komaleeswari K. Evaluation of antimicrobial activity of methanolic extract fractions of Delonix elata bark. Int J Res Ayurveda Pharm 2012;3:425-7.
Gayathri G, Saraswathy A, Vijayalakshmi K. Anti-microbial activity of medicinal plant Bauhinia variegata Linn. Int J Pharma Bio Sci 2011;1:400-8.