• CHAMPA KUMARI Malik Deenar College of Pharmacy, Seethangoli, Kasaragod, Kerala
  • UMADEVI A. Malik Deenar College of Pharmacy, Seethangoli, Kasaragod, Kerala
  • P. AJITH KUMAR Malik Deenar College of Pharmacy, Seethangoli, Kasaragod, Kerala




Actinomycetes, Rhizosphere, Ocimum sanctum, Plant growth promoter, Secondary metabolite


Objective: The study was aimed to isolate and identify Actinomycetes from rhizosphere soil of Malik deenar college of pharmacy, Kasaragod, Kerala, and to screen the plant growth promoting the activity of isolated actinomycetes on Ocimum sanctum.

Methods: The Actinomycetes were isolated from rhizosphere region soil by serial dilution and pour plating method. Then they were identified to a generic level based on morphological characters, and biochemical characters. Plant growth promoting activity was screened by placing 3 sets of Ocimum sanctum seedlings in different pots and the shoot length was measured after 10 d and compared with control.

Results: 19 different types of isolates were chosen based on their morphological characteristics and used for assessments of plant growth promoting activity. Among the 19 isolates, 17 isolates were identified as Streptomyces spp. And 2 were belongs to Nocardia spp. The actinomycetes isolates which belong to Streptomyces spp were shown plant growth promoting activity.

Conclusion: The result of this study revealed that the rhizosphere soil has different types of actinomycetes, among these Streptomyces spp. are more abundant and common. These Streptomyces spp. have predominant activity on plant growth promotion with respect to Ocimum sanctum.


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1. Panhwar QA, Othman Rahman ZA, Meon S, Ismail MR. Isolation and characterization of phosphate solubilizing bacteria from aerobic rice. Afr J Biotechnol 2012;11:6701-50.
2. Sousa CS, Soares ACF, Garrido MS. Characterization of streptomyces with potential to promote plant growth and biocontrol. Scientia Agricola 2008;65:50-5.
3. Ilic SB, Konstaninovic SS, Todorovic ZB, Lazic ML, Veljkovic VB, Jokovic N, et al. Characterization and antimicrobial activity of the bioactive metabolites in streptomyces isolates. Microbiology 2007;76:421-8.
4. Carla de Silva, Ana Christiana, Fermino Soares, Mrlon de Silva Garnido. Characterization of streptomyces with potential to promote plant growth and biocontrol. Agric 2008;65:50 5.
5. Gautham SA, Shobha KS, Onkarappa R, Prashith Kekuda TR. Isolation, Characterization and antimicrobial potential of streptomyces species from western ghat of Karnataka, India. Res J Pharm Technol 2012;5:974-3678.
6. Franco Correa M, Quintana A, Duque C. Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Appl Soil Ecol 2010;45:209-17.
7. Aneja KR. Culture media. In: Experiments in microbiology, plant pathology and biotechnology. (4th edition), new age international pvt. Ltd, New Delhi; 2007. p. 102, 106, 246-274.
8. Cappuccino, Sherman. Microbiology a laboratory manual. P. Fourth edition. The Benjamin/cummings publication company Inc; 1996. p. 20-30.
9. Gopala Krishna S, Humayun P, Srinivas V, Vijayabharathi R, Bhimineni RK, Rupela O. Plant growth promoting traits of streptomyces with biocontrol potential isolated from herbal vermicomposting. Biocontrol Sci Technol 2012;22:1199-210.
10. Holt JG, Krieg RN, Sneath PHA, Staley JT, Williams ST. Bergeys manual of determinative Bacteriology, Lippincott Williams and Wilkins, Baltimore, Maryland, 9th edn; 2000.
11. Merzaeva OV, Shirokikh IG. Colonization of plant rhizosphere by actinomycetes of different genera. Microbiology 2006;75:226-30.
12. Shirling EB, Gottlieb D. Methods for characterization of streptomyces species. Int J Syst Bacteriol 1996;16:313-40.



How to Cite

KUMARI, C., . U. A., and P. A. KUMAR. “EFFECT OF ACTINOMYCETES ON GROWTH OF OCIMUM SANCTUM”. International Journal of Current Pharmaceutical Research, vol. 11, no. 3, May 2019, pp. 45-47, doi:10.22159/ijcpr.2019v11i3.34096.



Original Article(s)