DEVELOPMENT AND EVALUATION OF POLYHERBAL GEL FOR ANTIFUNGAL ACTIVITY

  • Umadevi A. Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India
  • Champa Kumari Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India
  • P. Ajith Kumar Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India
  • Hameemath Shamana Nasmin Am Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India
  • Divya K. Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India
  • Hisana P. V. Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasaragod, Kerala, India

Abstract

Objective: Piper betal and Piper nigrumare traditional medicinal plants that have antifungal activity against Candida albicans, a combination of these two plants have not been known for its activity against this fungus. The purpose of this research was to formulate topical gel, a combination of P. betal and P. nigram which has antifungal activity against Candida albicans.

Methods: The antifungal activity test of P. betal and P. nigrum using agar well diffusion method was carried out. Thereafter, a topical gel formulation was prepared using Sodium carboxymethyl cellulose as a gelling agent of concentration 1; 1.5 and 2%. Test parameters for topical gel includes organoleptic, pH, extrudability, spreadability, diffusion, and stability test.

Result: The results of this study showed that P. betel and P. nigrum extracts had antifungal activity. Antifungal activity combination of P. betel and P. nigrum leaf extract is synergistic. For the formulation materials, the concentration chosen is 1: 1 (P. betel: P. nigrum) because in that combination the value of the resistor area is still categorized well. The stability test results stated that all the formulas were stable even after 30 d of stability studies.

Conclusion: This is the first report on the scientific evaluation of betel and pepper leaf extracts combination as a gel for antifungal activity. Thus our study reveals both leaf extracts to be good antifungals; their methanolic hydro extracts may be formulated as hydrogels with satisfactory physicochemical parameters.

Keywords: Piper betal, Piper nigrum, Antifungal activity, Candida albicans, Gel

References

1. De PV, Mark N, Abel BL. Synthesis and fungicidal, insecticidal activity of new amide derivatives of piperine. Pest Management Sci 2000;56:168-74.
2. Das K, Dang R, Machale M. Formulation and evaluation of a novel gel of stevia extract. Iranian J Dermatol 2009;12:117-22.
3. Gouttum Misra DS, Gopalakrishna Pillai GK. Polyherbal formulation encapsulated in phospholipids for the hypoglycemic activity of Momordicacharantia, Trigonellafoenum-graecum, and Withaniasomnifera. J Ethnopharmacol 2017;5:108-547.
4. G Sharmila Banu, G Kumar, AG Murugesan. Antihyperglycemic effect of Garlip, a poliherbal formulation in induced diabetics in rats. Food Chem Toxicol 2009;47:2361-5.
5. Zoheir A Damanhouri, Aftab Ahmad. A review on the therapeutic potential of Piper nigrum L. (Black Pepper): King Spices 2014;3:1-6.
6. Amonkar AJ, Nagabhushan M, D‟Souza AV, Bhide SV. Hydroxychavicol: a new phenolic antimutagen from betel leaf. IARC Sci Publications 1991;105:520-4.
7. Madhavi B, Nath AR, Banji D, Madhu MN, Ramalingam R, Swetha D. Extraction, identification, formulation and evaluation of piperine in alginate beads. Int J Pharm Pharm Sci 2009;1:156-8.
8. Ahmad N, Fazal H, Abbasi BH, Farooq S, Ali M. Biological role of Pipernigrum L. (Black pepper): a review. Asian Pacific J Tropical Biomed 2012;2:1945-53.
9. Aiyalu R, Govindarajan A, Ramaswami A. Formulation and evaluation of herbal gel for the treatment of arthritis in an animal model. Brazilian J Pharm Sci 2016;52:493-507.
10. Kaur LP, Garg R, Gupta GD. Development and evaluation of topical gel of minoxidil from different polymer bases in the application of alopecia. Int J Pharm Pharm Sci 2010;2:43-7.
11. Mahmoud DA, Hassanein NM, Youssef KA, Abouzeid MA. Antifungal activity of different Neem leaf extracts and the nimonol against some important human pathogens. Brazilian J 2011;42:1007-16.
12. Velraj M, Soumya D, Sndhukavi D. Antibacterial and antifungal activity of herbal gel from the ethanolic extract of stem bark of Bauhinia variegate Linn. Int J Pharm Sci Rev Res 2016;41:53-6.
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How to Cite
A., U., C. Kumari, P. A. Kumar, H. S. Nasmin Am, D. K., and H. P. V. “DEVELOPMENT AND EVALUATION OF POLYHERBAL GEL FOR ANTIFUNGAL ACTIVITY”. International Journal of Current Pharmaceutical Research, Vol. 10, no. 5, Sept. 2018, pp. 40-43, doi:10.22159/ijcpr.2018v10i5.29694.
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