PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF AZADIRACHTA INDICA AND PLECTRANTHUS AMBOINICUS EXTRACT
Keywords:Herbal plant, Antimicrobial activity
Objective: In the present research, a clear systematic investigation of phytochemical screening and antibacterial activity of herbal plants such as Azadirachta indica and Plectranthus amboinicus has been carried out.
Methods: The aqueous and alcoholic extract was prepared in soxhlet apparatus and phytochemical analysis of extracts was performed and analysed. The in vitro antimicrobial activity was performed by cup plate method. These extracts were studied under agar diffusion method against three bacterial species such as Bacillussubtills, Staphylococcus aureus, and Escherichia coli at 5µg, 50 µg and 250 µg concentration.
Results: The combine extract showed a predominant activity against these bacteria, which confirmed antimicrobial activity in AEAI and AEPA
Conclusion: The results obtained in this study clearly indicate that AEAI and AEPA has a significant potential to use as an antimicrobial agent
2. D Ragunath. Emerging antibiotic resistance in bacteria with special reference. Indian J Biosci 2008;33:593-603.
3. Venugopalan Santhosh Kumar, Visweswaran Navaratnam, Neem (Azadirachta indica). Prehistory to contemporary medicinal uses to humankind; 2013. p. 505–14.
4. lRamadass N, Subramanian N. Study of phytochemical screening of neem (Azadirachta indica). Int J Zool Studies 2018;3:209-12.
5. Porter, Anna Horsbrugh. Neem: India's tree of life, BBC; 2006.
6. Jairajpuri S, Zillur Rahman, M Shamim SL. Neem in unani medicine. Neem in unani medicine. India New Delhi; 1993.
7. Agrawal DP. Medicinal properties of Neem: New Findings; 1992.
8. Roshan Patel, Naveen Mahobia, Naheed Waseem, Nitin Upwar. Phyto-physicochemical investigation of leaves of plectranthus amboinicus. Pharmacogn J 2010;2:25-7.
9. Overlay Panel Seham S, El-hawary Rabie H, El-sofany Azza R, Abdel Monem Rehab S, Ashour. Phytochemical screening, DNA fingerprinting, and nutritional value of plectranthus amboinicus (Lour.). Spreng 2012;4:20-30-32.
10. Whistler WA. Tropical ornament SL: Timber Press Portland, Oregon USA; 2000.
11. Perez C, Pauli M, Bazerqu. An antibiotic assay by the well agar method. Acta Biol Med Exp 1990;15:13-5.
12. Shukla, Nayan R, Bhalodia VJ. Antibacterial and antifungal activities from leaf extracts of cassia fistula l.: an ethnomedicinal plant. J Adv Pharm Technol Res 2011;2:104–9.
13. Choudhary, Vijay. Description and medicinal uses of azadirachta Indica (Neem); 2019.
14. Khan, SS Rao, S Darsipudi, SG Divya, H Amaranad. Phytochemical studies and screening of leaves extracts of azadirachta indica for its anti-microbial activity against dental pathogens. IJCP 2010;2:23-6.
15. C Koti, Aparna Gore, AHM Thippeswamy, AHM Viswanatha Swamy, Rucha Kulkarni. Alcoholic leaf extract of plectranthus amboinicus regulates carbohydrate metabolism in alloxan-induced diabetic rats. Indian J Pharmacol 2011;6:32-6.
16. B Jayalakshmia, KA Raveeshab, M Muralic, KN Amruthesh. Phytochemical, antibacterial and antioxidant studies on leaf extracts of piper betle l. Int J Pharm Pharm Sci 2015;7:10.
17. Panel Zainab, Saif Saleh, Al-Hashemi Mohammad. Biological activities of different neem leaf crude extracts used locally in Ayurvedic medicine. Pacific Sci Rev: Nat Sci Eng 2016;18:128-31.
18. https://www.webmd.com/vitamins/ai/ingredientmono-577/neem. [Last accessed on 10 Aug 2019]
19. Sharada L Deore, Khadabadi Lalita, Bhagure SS, DS Ghorpade. In vitro antimicrobial and antioxidant studies on Enicostemma axillare (Lam) Raynal leaves. Nat Prod Radiance 2008;7:409-12.