FORMULATION AND EVALUATION OF MICROEMULSION CONTAINING NEEM SEED OIL

  • YOGESH S. THORAT Department of Pharmaceutics, D. S. T. S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India
  • NAGESH S. KOTE Department of Pharmaceutics, D. S. T. S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India
  • VIRENDRA V. PATIL Department of Pharmaceutics, D. S. T. S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India
  • AVINASH H. HOSMANI Department of Pharmaceutics, Government College of Pharmacy, Ratnagiri

Abstract

Objective: The objective of the present study was to formulate Microemulsion containing seed oil. Neem seed (Azadirachtaindica) oil was extracted from its seeds by the soxhlet apparatus. Acetone is used as a solvent. PEG 400 and Carbopol 940p was select as surfactant, co-surfactant and hydrogel thickening agent. Microemulsions were characterized for pH, viscosity, spreadability, in vitro drug transport study and in vivo antibacterial activity and shows satisfactory results. Antibacterial activity of formulation against E. coli Shows at a concentration of 3%. The neem seed oil microemulsion has the potential for antibacterial activity.


Methods: A ratio of surfactant and cosurfactant i.e, S/CoSchoosen and corresponding mixture was made. The mixture was mixed with oil. Each mixture was mixed thoroughly using magnetic stirrer until homogenous dispersion/solution was obtained. Double distilled water was used in this formulations as to prevent the incorporation of surface active impurities. The mixture was titrated with water and ambient temperature with constant stirring at the endpoint where the mixture become clouded, the quantity of aquous phase added. The percentage of three different pseudo-phases incorporated were calculated. 


Results: Solubility studies in various solvents reveals that the oil is insoluble in distilled water and ethanol. Soluble in methanol.


Conclusion: It was observed that the microemulsion having multilamellar nature. Batches with carbopol shows better homogenous distribution. The stability of microemulsion prepared with carbopol 71 was gretter than with xanthan gum. The in vitro study of microemulsion was performed and Batch (F7) is optimized batch which shows highest drug release.

Keywords: Neem seed oil, Microemulsion, Topical application, Antibacterial activity

References

1. Vidya Sabale, Sejal Vora. Formulation and evaluation of microemulsion-based hydrogel for topical delivery. Int J Pharm Invest 2012;2:141-9.
2. Faizi Muzaffar, UK Singh. Design development and evaluation of topical microemulsion. Int Res J Pharm 2017;8:95-110.
3. Vidya Sabale, Sejal Vora. Formulation and evaluation of microemulsion-based hydrogel for topical delivery. Int J Pharm Invest 2012;2:140-9.
4. Muhammed Majeed, Satyan KS. Neem oil limonoids: product overview; 2007. p. 2-8.
5. Mohammad A. Review on spermicidal activity of azardirachta Indica. J Pharmacol Phytochem 2013;1:61-75.
6. Rodina Sadr Ghotbi, Marziyeh Khatibzadeh, Shadi Kordbacheh. Preparation of neem seed oil nanoemulsion. Proceedings of the 5th International Conference on Nanotechnology: Fundamentals and Applications Prague, Czech Republic; 2014;11:1-5.
7. BA Ikyenge, BO Ogenyi. An evaluation of the impact of epoxidized neem seed oil (azadirachtaindica) modification on the adhesive properties of soybean glue. Int J Adv Res Chem Sci 2015;2:1-5.
8. Dubhashi S, Pranay Singaiah M, Prasad V. V studies on extraction and HPLC analysis of azadirachtin from kernels of neem seed. J Adv Pharm Edu Res 2013;3:57-60.
9. Patel J, Trivedi J. Formulation and evaluation of microemulsion based topical hydrogel containimg Lornoxicom. J Appl Pharm Sci 2014;4:625-38.
10. Walekar SS, Wankkhade NB. Microemusion based gel system: anovel approach for topical drug delivery. Int J Adv Pharm Sci 2014;5:1776-82.
Statistics
22 Views | 9 Downloads
Citatons
How to Cite
THORAT, Y. S., N. S. KOTE, V. V. PATIL, and A. H. HOSMANI. “FORMULATION AND EVALUATION OF MICROEMULSION CONTAINING NEEM SEED OIL”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 3, May 2020, pp. 31-36, doi:10.22159/ijcpr.2020v12i3.38301.
Section
Original Article(s)