EFFECT OF LUTEIN IN HYPERCHOLESTEROLEMIA INDUCED OXIDATIVE STRESS IN MALE WISTAR RATS
Keywords:Hypercholesterolemia, Oxidative stress, Liver, Lutein
Objective: Oxidative stress induced by reactive oxygen species (ROS) plays an important role in the etiology of several diseases, including atherosclerosis and coronary heart disease. Hypercholesterolemia is reported to be associated with the oxidative stress that results from the increased production of ROS or impairment of the antioxidant system. Hence the objective of this study was to evaluate the antioxidant effect of lutein in hypercholesterolemiainduced oxidative stress in male wistar rats.
Methods: Male Wistar rats were divided into 6 groups of 6 each. Group I served as control. Group II III, IV, V & VI rats were received high cholesterol diet. Group III was treated with Atorvastatin 5 mg/kg. Group IV, V & VI rats was treated with 25 mg/kg, 50 mg/kg & 100 mg/kg of Lutein. After 16 weeks, blood samples &liver tissue samples were collected from all the groups of animals to evaluate antioxidant levels in blood & tissue samples.
Results: Catalase, superoxide dismutase, glutathione peroxidase, glutathione levels significantly increased in both plasma & liver in lutein treated groups. TBARs level is increased in plasma in hypercholesterolemicrats and Malondialdehyde level in the liver tissue is also significantly increased in hypercholesterolemic rats.
Conclusion: The results of this study indicate Lutein is an effective nutritional supplement to prevent oxidative stress in hypercholesterolemia.
Libby P. The pathogenesis, prevention and treatment of Atherosclerosis. In: Harrisons Principles of Internal Medicine. Eds. Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson JL, Loscalzo J. 18th ed. McGraw-Hill Book Co. Newyork, 2012;241:1983-91.
Chisolm GM. The oxidative modification hypothesis of atherogenesis: an overview. Biol Med 2001;28:1815â€“26.
Valko M. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44â€“84.
Reieckschloss U, Galle J, Holtz J. Induction of NADPH oxidase by oxidized LDL in human endothelial cells: antioxidative potential of HMG-Coenzyme A reductase inhibitor therapy. Circulation 2001;104:1767-72.
Antoniades C, Tousoulis D, Stefanadis C. Effect of endothelial nitric oxide synthase gene polymorphisms on oxidative stress, inflammatory status and coronary atherosclerosis: an example of transient phenotype. J Am Coll Cardiol 2007;49:1226.
Bourcier T, Sukhnowa G, Libby P. The nuclear factor of kappa Î² signalling pathway participates in dysregulation of vascular smooth muscle cells in vitro and in human atherosclerosis. J Biol Chem 272:15817-24.
Lonn EM, Yusuf S, Dzavik V. Effects of rampril and vitamin E on atherosclerosis: the study to evaluate carotid ultrasound changes in patients treated with rampril and vitamin E. Circulation 2001;103:1059-63.
Singh U, Devaraj S, Jialal I. Vitamin E, oxidative stress and inflammation. Annu Rev Nutr 2005;25:151-74.
Witzum J, Steinberg D. Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest 1991;88:1785-92.
Asokan BR, Jaikumar S, Ramaswamy S, Thirugnanasambandam P, Nirmala P. Anti-hyperlipidemic activity of a poly herbal formulation in experimental models. Pharmacologyonline 2010;433-42.
Hao Han, Wei Cui, Danhong Gao, Bingquing Huang, Lin Fan. Oxygenated carotenoid Lutein supplementation against early atherosclerosis in apo-deficient mice through alleviation the dyslipidemia and attenuation the oxidative stress.
Nicholas S Brown, Roy Bicknell. Hypoxia and oxidative stress in breast cancer: Oxidative stress: its effects on the growth, metastatic potential and response to therapy of breast cancer. Breast Cancer Res 2001;3(5):323â€“7.
Girotti J. Mechanisms of lipid peroxidation. Free Radicals Biol Med 1985;1:87-95.
Kyoto Nishi, Masaa Killno, Kenji fukuzawa. Cllinico pathological significance of lipid peroxidation in carotid plaques. Atherosclerosis 2002;160(2);289-96.
Philippe Becuwe, Marie Ennen, Remiklotz Claire Barbieux, Stephanie grandemange. Manganese superoxide dismutase in breast cancer:-from molecular mechanisms of gene regulation to biological & clinical significance. Free Radical Biol Medâ€ 2014;77:139-51.
Tsutomu Tamai, Hirofumi Uto. Serum manganese superoxide dismutase & thioredoxin are potential prognostic markers for hepatitis C virus related hepatocellular carcinoma. World J Gasteroenterol 2011;17(44):4890-8.
Hosono M, Iwamoto N, Hayashi H, Takenchi N, Iwamura J. Measurement of serum catalase activity and its clinical significance Rinshi Byoriâ€ 1996;44(5):444-8.
Xiang Qi, Kevin Tan Pan Ng, Qi Jhonlian, Xiao Bing Liu, Chang Xian Li, Wei gengchang, et al. Clinical significance & therapeutic value of glutathione peroxidase 3 (Gpx 3) in hepatocellular carcinoma Oncotargetâ€; 2014.
Davey MW, Stals E, Panis B, Keulemans J, Swennen RL. High-throughput determination of malondialdehyde in plant tissuesâ€ Anal Biochem 2005;347(2)201-7.
Pryor WA, Stanley JP. Letter: A suggested mechanism for the production of malondialdehyde during the antioxidant of polyunsaturated fatty acids. Non enzymatic production of prostaglandin endoperoxidase during autoxidation. J Org chem 1975;40(24):3615-7.
Farmer EE, Davoine C. Reactive electrophile species. Curr Opin Plant Boil 2007;10(4);380-6.
Marnett LJ. Lipid peroxidationâ€“DNA damage by malondialdehyde. Mutat Res 1999;424(1-2);83-95.
Tiku ML, Narla H, Jain M, Yalamanchili P. Glucosamine prevents in vitro collagen degradation in chondrocytes by inhibiting advanced lipoxidation reactions & protein oxidation. Arthritis Res Ther 2007;9(4)R76.
Buddi R, Lin B, Atilano SR, Zorapapel NC, Kenney MC, Brown DJ. Evidence of oxidative stress in human corneal diseaseâ€ J Histochem cytochem 2002;50(3):341-51.
Nair V, Oâ€™Neil LC, Wang PG. Malondialdehydeâ€™ Encyclopedia of reagents for organic synthesis John Wiley & Sons: New York; 2008.