EXTRA VIRGIN OLIVE OIL EFFECT IN THE COMBINATION OF ALCOHOL AND BENZENE ON THE KIDNEY OF RATTUS NOVERGICUS

ADETUNJI OPEYEMI ADEBOLA; ADETUNJI OLUWASEYI ADEGOKE; NWOBI JOSEPH CHIGBOGU; ADETUNJI IYABODE TOYIN; OGUNBIYI BABAFEMI TOSIN; NWOBI NNENNA LINDA

doi:10.22159/ijms.2021.v9i6.43029

Authors

ADETUNJI OPEYEMI ADEBOLA Department of Anatomy, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
ADETUNJI OLUWASEYI ADEGOKE Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
NWOBI JOSEPH CHIGBOGU Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
ADETUNJI IYABODE TOYIN Department of Microbiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
OGUNBIYI BABAFEMI TOSIN Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.
NWOBI NNENNA LINDA Department of Chemical Pathology, School of Basic Clinical Sciences, Benjamin S. Carson (Snr.) College of Health and Medical Sciences, Babcock University, Ogun State, Nigeria.

DOI:

https://doi.org/10.22159/ijms.2021.v9i6.43029

Keywords:

Ethanol, Benzene, Extra virgin olive oil, Nephron

Abstract

Objective: This study investigated the use of extra virgin olive oil (EVOO), which is said to possess anti-inflammatory components as an ameliorative substance for the induced inflammation.

Methods: Forty-eight female Wistar rats were used for this study. They were divided into eight (1–8) groups (n=6); Control, Ethanol, Benzene, E+B, E+O, B+O, E+B+O with the administration of 25% Ethanol, 200 mg/kg/b.w Benzene, 2 ml (O) EVOO in respect to the tagged grouping names. Animals were euthanized through cervical dislocation after the last day of administration and the liver was excised and part was fixed in formalin solution of 10% for histological processing and the other part was homogenized for biochemical assay in phosphate buffer before centrifugation.

Results: Histological evidence creates an understanding of the nephrons in which benzene and ethanol manifest likewise the mitigated ability of EVOO. The relative organ weight provides information on the extent of the damage on nephrons. The kidney test level is significantly expressed.

Conclusion: Ethanol and benzene in combination cause serious damage; also, they separately induce nephrons dysfunction. EVOO was shown to mitigate these nephrons damages.

References

Linlin C, Huidan D, Hengmin C, Jing F, Zhicai Z, Junliang D, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2017;9:7204-18.

Takeuchi O, Akira S. Pattern recognition receptors and inflammation. Cell 2010;140:805-20.

Hörl WH. Nonsteroidal anti-inflammatory drugs and the kidney. Pharmaceuticals (Basel) 2010;3:2291-321.

Neghab M, Hosseinzadeh K, Hassanzadeh J. Early liver and kidney dysfunction associated with occupational exposure to sub-threshold limit value levels of benzene, toluene, and xylenes in unleaded petrol. Saf Health Work 2015;6:312-6.

Saeed A. Ethanol-induced hepatic and renal histopathological changes in BALB/c mice. J Natural Sci Res 2015;5:12-20.

Adebola AO, Adegoke AO, Toyin AI, Linda NN. Role of extra virgin olive oil on the liver of alcohol and benzene-treated Wistar rats. World J Med Sci 2021;18:51-8. Available from: https://www.idosi.org/ wjms/18(2)21/2.pdf

Ying Z, Yan H, Haihua H. Triptolide attenuates inflammatory response in membranous glomerulo-nephritis rat via downregulation of NF-κB signaling pathway. Kidney Blood Press Res 2016;41:901-10.

Suryanarayana V, Vulimiri, Mahedevan B, Reproductive Developmental Toxicity of Solvents and Gases. 2nd ed. Silo. Pub; 2017. p. 1409-46.

Abel EL, DiGiovanni J. The Molecular Basis of Cancer. 4th ed., Vol. 7. Amsterdam, Netherlands: Elsevier; 2015. p. 103-28.

Das S, Vasudevan D. Alcohol induced effects on kidney. Indian J Clin Biochem 2008;23:4-9.

Tajmim A, Siddique AB, El Sayed K. Optimization of taste-masked (-)-oleocanthal effervescent formulation with potent breast cancer progression and recurrence suppressive activities. Pharmaceutics 2019;11:515.

Dhifi W, Khedher MB, Bellili S, Sadaka C, Wakim L, Beyrouthy ME, et al. Effects of olive drying and storage on the oxidative status, aroma, chlorophyll and fatty acids composition of olive oil. Int J Pharm Pharm Sci 2015;7:102-8. Available from: https://www.innovareacademics.in/ journals/index.php/ijpps/article/view/1991

Giri D. Periodic Acid Schiff (PAS) Staining Technique for Carbohydrates; 2019. Available from: https://www.laboratoryinfo.com/ periodic-acid-schiff-pas-staining-technique-for-carbohydrates

Giri D. Hematoxylin and Eosin Staining: Principle, Procedure and Interpretation; 2019. Available from: https://www.laboratoryinfo.com/ hematoxylin-and-eosin-staining

Laaboudi W, Ghanam J, Ghoumari O, Sounni F, Merzouki M, Benlemlih M. Hypoglycemic and hypolipidemic effects of phenolic olive tree extract in streptozotocin diabetic rats. Int J Pharm Pharm Sci 2016;8:287-91.

Paidesetty SK, Rout SK, Sahoo J. Antimicrobial and antioxidant activities of some newly synthesized benzene-1, 3-diol congeners and their characterizatio. Int J Pharm Pharm Sci 2015;7:427-33.