MORAXELLA OSLOENSIS MEDIATED SYNTHESIS OF TIO2 NANOPARTICLES
Keywords:
Moraxella osloensis, biogenesis, TiO2 NP, characterization, cytotoxicityAbstract
Objective: The main idea behind the present investigation is to explore the ability of Moraxella osloensis to reduce Titanium dioxide to nanosizes in a facile manner.
Methods: The bacterium was grown in nutrient broth for 24 hr. The culture supernant was used for the bioreduction process. The synthesized nanoparticles were characterised by instrumental analyses namely FTIR, SEM and XRD. Toxicity of the nanoparticles was tested against HaCaT and Hep2 cell lines.
Results: Moraxella osloensis with the dye degrading ability was found to be capable of reducing bulk Titanium dioxide to nanostructure. These biogenic TiO2 nanoparticles (TiO2 NPs) were in the size range of 60–150 nm with the average size of 72 nm. The particles were found to irregularly shaped and uniformly dispersed with less aggregation. In addition, XRD analysis indicated the presence of titanium beta. FTIR confirmed the involvement of proteins in the bioreduction and stabilization. Nano TiO2 was found to be more toxic against HaCaT and Hep2 cell lines than its bulk counterpart.
Conclusion: Moraxella osloensis successfully synthesised TiO2 NPs in environmentally safe and cost effective method in an extracellular fashion.
Keywords: Moraxella osloensis, Biogenesis, TiO2 NP, Characterization, Cytotoxicity
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