METHOD DEVELOPMENT FOR QUANTIFICATION OF OXIDATION COMPLEXES OF NADOLOL AND RESVERATROL BY VISIBLE SPECTROPHOTOMETRY
Keywords:
Nadolol (NAD), Resveratrol (RES), 2, 2-bipyridyl and ferric chlorideAbstract
Objective: The present work is a report on two colorimetric methods based on oxidation and complexation of nadolol and resveratrol with ferric chloride and 2, 2'-bipyridyl.
Methods: The methods were developed for Perkin Elmer LAMBDA 25 UV –VIS spectrophotometer with 1 cm matched quartz cells. The functional groups vulnerable to oxidation were oxidized by ferric chloride and the reduced ferrous salts were complexed with 2, 2'-bipyridyl. The reaction conditions were optimized and validated to achieve maximum color intensity.
Results: The colored complexes show maximum absorbance measured at 424 nm for NAD and 522 nm for RES. The absorbance was found to increase linearly with an increase in concentration which was corroborated by the calculated regression coefficients (0.9998-0.9999). Linearity was obeyed in the range 5-25 and 20-100 µg/ml for RES and NAD, respectively. The molar absorptivity, sandell's sensitivity, LOD, LOQ and other validation parameters have been evaluated extensively as per ICH guidelines and all the parameters were found within the acceptance criteria for all the methods.
Conclusion: The proposed methods were proven to be more accurate, simple, precise and rapid by statistical validation and recovery studies; hence can be used for routine laboratory analysis.
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References
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