• K. i. Young Shin
  • Beom Young Won
  • Hyun Jee H. a. Braincell LaboratoryCo. Ltd,Seoul, South Korea
  • Yeo Sang Yun
  • Keun a. Chang
  • Hyung Gun Lee
  • Myeong Hwan O. h. International Ginseng and Herb Research Institute,Geumsan, Korea
  • Hwan Lee
  • Young Sik Park
  • Jong Dae Park
  • M. i. Kyung Pyo International Ginseng and Herb Research Institute, Geumsan, Korea


Ginseng, Nil, Acetylcholinesterase, Ultrafiltration, Memory


Objective: The goal of this study was to research for an effective fraction on memory improvement of Korean red ginseng.

Methods: In this study, 80 % ethanol red ginseng extract (RE) was divided into inner fluid (REUI) and outer fluid (REUO) by the ultrafiltration and then REUO was further separated into four fractions namely, REUO-00, REUO-30, REUO-50 and REUO-70, respectively, by Diaion HP-20 column chromatography.

Results: REUO has protected more significantly the H2O2-induced SHSY-5Y cell death than REUI. Interestingly, the hydrophobic parts of the REUO (REUO-EtOHs) such as REUO-30,-50 and-70 decreased more significantly the H2O2-induced cell death than its hydrophilic part (REUO-00) in a dose-dependent manner. Then, we focused on the activity of a candidate for cholinergic functions, because memory deficits of neurodegenerative diseases are closely associated with cholinergic dysfunctions. The REUO-EtOHs (1.25 mg/ml) inhibited the activity of the acetylcholinesterase and its half maximal inhibitory concentration (IC50) was about 2.358 mg/ml. Additionally, we investigated whether the intake of the REUO (50 mg/kg/d) during 12 w could improve memory impairment of 12-month old Tg2576 mice and decrease total soluble amyloid-β (Aβ) proteins in the mouse brain cortex. The REUO alleviated significantly the memory impairment and successfully reduced the levels of the soluble Aβ proteins in the mouse cortex.

Conclusion: We finally suggest that the REUO, including majorly its hydrophobic part that may be considered as more effective for memory improvement, will be highly considered as valuable candidate for the memory-enhancing ingredients against cholinergic dysfunctions and cognitive impairments of neurodegenerative diseases including Alzheimer's disease.

Keywords: Ginseng, Alzheimer's disease, Acetylcholinesterase, Ultrafiltration, Memory



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How to Cite

Shin, K. i. Y., B. Y. Won, H. J. H. a., Y. S. Yun, K. a. Chang, H. G. Lee, M. H. O. h., H. Lee, Y. S. Park, J. D. Park, and M. i. K. Pyo. “ULTRAFILTRATED FRACTION OF KOREAN RED GINSENG EXTRACT IMPROVES MEMORY IMPAIRMENT OF TG2576 MICE VIA INHIBITION OF SOLUBLE AÎ’ PRODUCTION AND ACETYLCHOLINESTERASE ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 4, Apr. 2016, pp. 272-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/10684.



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