EVALUATION OF STRESS ALLEVIATING POTENTIAL OF PERGULARIA DAEMIA ON CERTAIN IMMUNOLOGICAL PARAMETERS
Keywords:
Stress indices, Immunomodulatory, bioactive compounds, Pergularia daemiaAbstract
Objective: Pergularia daemia [PD] Forsk, commonly called Veliparuthi†in Tamil belongs to the family of Asclepiadaceae. It has been used by villagers as traditional folk medicine for various ailments such as antipyretic, anti-venom, emmenagogue, anti-helminthic, and to treat malarial fever, infantile diarrhea. Scientific reports revealed several pharmacological properties of PD extract. Preliminary in vitro studies on the plant revealed the antioxidant, anti-inflammatory and antimicrobial activities of the plant. So the present study planned to investigate the in vivo stress alleviating the potential of  PD extract on noise stress induced model of wistar albino rats.
Methods: Adult Wistar albino rats were exposed to pure tone noise stress for 45 min for one day and the effect of ethanolic PD extract on plasma corticosterone, organ weight body weight ratio of lymphoid organs and adrenal gland,immunological parameters like total leucocyte count, differential leukocyte, neutrophil adherence test and candida phagocytosis was studied.
Results: Results revealed that acute stress significantly increased the plasma corticosterone level [p<0.001] and decreased the organ weight body weight ratio of the spleen [p<0.001] and thymus [p<0.01]. It also significantly decreased the total leucocyte count [p<0.001], percentage of neutrophils [p<0.001], eosinophils [p<0.004] and lymphocytes [p<0.001], neutrophil adherence [p<0.001] and enhanced the phagocytic index and avidity index [p<0.001] in acute noise stress exposed groups. Administration of PD extract significantly prevented those acute noise stress-induced changes [p<0.001].
Conclusion: We can be  PD extract protected against the noise stress induced changes in albino rats and this stress alleviating and immunomodulatory potential of the plant may be attributed to the phytochemicals and bioactive compounds present in it.
Keywords: Stress indices, Immunomodulatory, bioactive compounds, Pergularia daemiaÂ
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References
Gaurav Akrani. What is Stress? Meaning, Definition and Causes of Stress. Available from: http://kalyan-city.blogspot.com/ 2011/03/what-is-stress-meaning-definition-and. html. [Last accessed on 01 Jan 2016].
Ravindran R, Devi RS, Samson J, Senthilvelan M. Noise stress induced brain neurotransmitter changes and the effect of Ocimum sanctum Linn treatment in albino rats. J Pharmacol Sci 2005;98:354-60.
Berguland B, Lindvall T. Community noise. Archives of the center for sensory. Reseh Stocholm 1995;2:1-195.
Spreng M. Possible health effects of noise-induced cortisol increase. Noise Heath 2000;2:59-63.
Archana R, Namasivayam A. Effect of Ocimum sanctum on chronic noise on neutrophil functions in rats. Am Int J Res Formal Appl Nat Sci 2013;3 Suppl 1:88-92.
https://in.pinterest.com/pin/400961173052187434. [Last accessed on 01 Jan 2016].
Bhaskar VH, Balakrishnan N. Veliparuthi Pergularia daemia Forsk.–As a phytomedicine: a review. Int J Pharm Tech Res 2009;1 Suppl 4:305-13.
Suresh kumar SV, Mishra SH. Hepatoprotective activity of extracts from Pergularia daemia Forsk. against carbon tetrachloride-induced toxicity in rats. J Ethnopharmacol 2006;107:164-8.
Thatoi HN, Panda SK, Rath SK, Dutta SK. Antimicrobial activity and ethnomedicinal uses of some medicine plant from similipal biosphere reserve, Orissa. Asian J Plant Sci 2008;7 Suppl 3:260-7.
Golam S, Gafur MA, Shah MAB, Khurshid AHM, Biswas MHU, Hassan P, et al. Antifertility activity of Pergularia daemia. J Sci 2001;1:22-4.
Bhavin V, Ruchi V. Diuretic potential of whole plant extracts of Pergularia daemia Forsk. Iran J Pharm Res 2011;10 Suppl 4:795-8.
Ramya Sravani KM, Ashok Kumar CK, Mohanalakshmi S, Silpa Rani G. Psychopharmacological profiles of Pergularia daemia forsk Chiov. Asian J Pharm Clin Res 2012;5 Suppl 2:112-4.
Sridevi G, Sembulingam K, Prema Sembulingam, Muhammed Ibrahim, Srividya S. Evaluation of in vitro anti-inflammatory activity of Pergularia daemia. World J Pharm Res 2015;4 Suppl 6:1100-8.
Feldman S, Conforti N. Amygdalectomy inhibits adrenocortical responses to somatosensory and olfactory stimulation. Neuroendocrinology 1981;32 Suppl 6:330–4.
Mattingly D. A simple fluorimetric method for the study of free H-hydroxy corticosteroids in human plasma. J Clin Pathol 1962;15:374–9.
Archana R, Impact of noise stress on the immune status of albino rats. Res J Pharm Biol Chem Sci 2013;4 Suppl 4:713-7.
Srikumar R, Jeya Parthasarathy N, Sheela Devi R. Immunomodulatory activity of triphala on neutrophil functions. Biol Pharm Bull 2005;28 Suppl 8:1398–403.
Archana R, Namasivayam A. Effect of Ocimum sanctum on noise induced changes in neutrophil functions. J Ethnopharmacol 2000;73:81-5.
Sembulingam K, Sembulingam P, Namasivayam A. Effect of Ocimum sanctum Linn on noise induced changes in plasma corticosterone level. Indian J Physiol Pharmacol 1997;41 Suppl 2:139-43.
Manikandan S, Srikumar R, Jeya PN, Sheela Devi R. Protective effect of acormus calamus linn on free radical scavengers and lipid peroxidation is discrete regions of the brain against noise stress exposed rat. Biol Pharm Bull 2005;28 Suppl 12:2327-30.
Sembulingam K, Sembulingam P, Namasivayam A. Antistressor effect of Ocimum sanctum linn on charges in organ weight of albino rats induced by acute noise stress. Biomedicine 1998;18 Suppl 1:31-5.
Spehner V, De Wazieres, Nicod L, Harraga S, Robert JF, Seilles E. Auditory stress induces changes in membrane functions of mouse peritoneal macrophages. Scand J Immunol 1996;44:643-7.
Hemantkumar A Thakur. Antimicrobial and antifungal activity of Cucumismelo l. [Cucurbitaceae] and Pergularia deamia forsk. [Asclepiadaceae] ethnomedicinal plants. Int J Bioassays 2015;4 Suppl 1:3661-5.
Savitha G, Selvamani S, Balamurugan S. Evaluation of the antimicrobial potential of various solvent extracts of Pergularia daemia [forsk.] Chiov. Leaves. Int J Sci Inventions Today 2014;3 Suppl 6:719-24.
Sridevi G, Prema Sembulingam, Sekar Suresh, Sembulingam K. Phytochemical analysis of Pergularia daemia for its bioactive components through gas chromatographic mass spectrometry [GCMS]. IOSR J Pharm 2014;4 Suppl 5:41-6.
Kassa Belay, Mesay Sisay. Phytochemical constituents and physicochemical properties of the medicinal plant (Moringa Oleifera) around bule hora. Int J Innovation Sci Res 2014;8 Suppl 1:88-98.