ANTI-PSYCHOTIC ACTIVITY OF PYRUS COMMUNIS JUICE
Objective: The present study aim to investigate the anti-psychotic potential of pyrus communis in the rodents.
Methods: The fresh juice of pyrus communis (Pear) was administered orally to rodents for 21 d and the anti-psychotic activity was assessed by in vitro methods viz ketamine induced stereotypic behaviour, pole climbing avoidance in rats and swim induced grooming behaviour experimental models. The biochemical estimation was done on 21 d.
Results: The different concentrations of fresh pyrus communis juice was assayed. When pyrus communis juice (PCJ) was administered chronically for 21 d remarkably decreased ketamine induced falling, head-bobbing, weaving and turning counts. Administration of Pear juice significantly delayed the latency time taken by the animals to climb the pole in Cook's pole climb apparatus. In swim induced grooming behaviour model, Pear juice significantly reduced swim induced grooming behaviour. Moreover, Pear juice significantly decreased the brain dopamine levels and inhibited acetyl cholinesterase activity. In the present study, Pear juice significantly enhanced reduced glutathione levels in the brains of mice, thereby reflecting enhanced scavenging of free radicals and in turn preventing occurrence of psychotic attack.
Conclusion: The present study revealed that pyrus communis juice possessed significant anti-psychotic activity.
2. Li X, Zhang JY, Gao WY, Wang Y, Wang HY, Cao JG, et al. Chemical composition and anti-inflammatory and antioxidant activities of eight pear cultivars. J Agric Food Chem 2012;60:8738-44.
3. Sharma K, Pasricha V, Satpathy G, Gupta RK. Evaluation of the phytochemical and antioxidant activity of raw Pyrus communis (l), an underexploited fruit. J Pharmacogn Phytochem 2015;3:46-50.
4. Parle M, Arzoo. Honey: a sweet way to cure psychosis. Eur J Biomed Pharma Sci 2016;7:479-86.
5. Parle M, Kadian R, Kaura S. Non-behavioral models of psychosis. Int J Pharm 2013;4:89-95.
6. Hashimoto A, Yoshikawa M, Niwa A, Konno R. Mice lacking D-amino acid oxidase activity display marked attenuation of ste-reotypy and ataxia induced by MK-801. Brain Res 2005;1033:210-5.
7. Parle M, Kadian R. Behavioral models of psychosis. Int J Pharm 2013;4:26-30.
8. Ingale SP, Kasture SB. Psychopharmacological profile of passiflora incarnata linn in mice. Int J Phytopharmacol 2012;3:263-8.
9. Schlumpf M, Lichtensteiger W, Langemann H, Waser PG, Hefti F. A fluorimetric micromethod for the simultaneousdetermination of serotonin, noradrenaline and dopamine in milligram amounts of brain tissue. Biochem Pharmacol 1974;23:2337-46.
10. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7:88-95.
11. Ellman G. Tissue sulphydryl groups. Arch Biochem Biophys 1959;82:70-3.
12. Boskovic M, Vovk T, Plesnicar BK, Grabnar I. Oxidative stress in schizophrenia. Curr Neuropharmacol 2011;9:301-12.
13. Ciobica1 A, Padurariu M, Dobrin I, Stefanescu C, Dobrin R. Oxidative stress in schizophrenia-focusing on the main markers. Psychiatr Danub 2011;23:237-45.
14. Byron KY, Tsung-Ung WW. Oxidative stress in schizophrenia: an integrated approach. Neurosci Biobehav Rev 2011;35:878-93.
15. Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: Its medicinal and pharmacological applications. Afr J Pure Appl Chem 2010;4:142-51.
16. Mueller L, Boehm V. Antioxidant activity of Î²-carotene compounds in different in vitro assays. Mol 2011;16:1055-69.
17. Jager AK, Saaby L. Flavonoids and the CNS. Mol 2011;16:1471-85.
18. Kale MA, Bindu SM, Khadkikar P. Role of antioxidants and nutrition in oxidative stree: a review. Int J Appl Pharm 2015;7:1-4.
19. Koppula S, Kumar H, More SV, Kim BW, Kim IS, Choi DK. Recent advances on the neuroprotective potential of antioxidants in experimental models of Parkinsonismâ€™s disease. Int J Mol Sci 2012;13:10608-29.
20. Kovacsova M, Barta A, Parohova J, Vrankova S, Pechanova O. Neuroprotective mechanisms of natural polyphenolic compounds. Activ Nerv Super Rediv 2010;52:181-6.
21. Nachum-Biala Y, Troen AM. B-vitamins for neuroprotection: narrowing the evidence gap. Int Un Biochem Mol Biol 2012;38:145â€“50.
22. RubeÅ¡a G, Gudelj L, Kubinska N. Etiology of schizophrenia and therapeutic options. Psychiatr Danub 2011;23:308-15.