GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM AQUEOUS EXTRACT OF BASELLA ALBA AND THEIR IN-VITRO ANTIOXIDANT POTENTIALS
Objective: To synthesize silver nanoparticles (AgNps) from Basella alba(B. alba) aqueous extract using 3 mM AgNO3 solution, to characterize the resulted AgNps and also to study the antioxidant activity by DPPH and reducing assay.
Methods: 150 ml of aqueous extract was mixed with 600 ml of 3 mM silver nitrate solutions and kept at room temperature for 1hr. A change from straw yellow to golden yellow was observed and absorption spectra were obtained by UV-VIS spectrometer. The resulted nanoparticles (NPs) were characterized by FT-IR spectrum, SEM-EDS, TEM and XPS techniques. The purity and nature of the particles were observed through TG-DSC analysis. Further, Antioxidant activity was performed by DPPH (2, 2-diphenyl-1-picrylhydrazyl) method and by Ferric chloride reducing assay.
Results: UV-Vis spectral analysis shows a maximum absorption peak at 435 nm. FT-IR spectrum indicated the formation of reducing and capping agents in AgNps. The synthesized silver nanoparticles was spherical in shape and its size ranges from 22.6 to 25 nm. The synthesized silver nanoparticles possess excellent antioxidant activity by various methods. The phenol carboxylic acid present in B. alba leaf extract acted as a reducing agent which was identified by HPTLC technique. A Protein present in the B. alba extract was responsible for the capping of bio-reduced silver nanoparticles.
Conclusion: It has been demonstrated that Basella alba plant extract could be used as a proficient green reducing agent for the synthesis of AgNPs. Further studies will be focused towards the mass scale production of formulation.
2. Prakash Somani R, Savita Somani P, Umeno M. Application of metal nanoparticles decorated carbon nanotubes in photovoltaics. Appl Phys Lett 2008;93:1-4.
3. Rajathi K, Sridhar S. Green synthesized silver nanoparticles from the medicinal plant wrightia tinctoria and itâ€™s antimicrobial potential. Int J Chemtech Res 2013;5:1707-13.
4. Maribel Guzman G, Jean Dille, Stephan Godet. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biol Eng 2009;2:104-11.
5. Hui-Jun Li, An-Oi Zhang, yang Hu, Li Sui, Dong-Jin Qian, Meng Chen. Large-scale synthesis and self-organization of silver nanoparticles with Tween 80 as a reductant and stabilizer. Nanoscale Res Lett 2012;7:612.
6. Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 2009;32:79-84.
7. Jagadish Chandra Tarafdar, Ramesh Raliya Rapid. Low-Cost and eco friendly approach for iron nanoparticle synthesis using aspergillus oryzae tfr9. J Nanopart 2013;2013:1-4.
8. Ponarulselvam S, Pannerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S. Synthesis of nanoparticles using leaves of catharanthus roseus linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed 2012;2:574-80.
9. Garimasinghal, Rhijubhavesh, Kunalkasariya, Ashishranjan Sharma. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopar Res 2011;13:2981â€“8.
10. Debabartbhasiya, NakulSharma, Rituparnabhora. Green synthesis of silver nanoparticles using Bryophyllum pinnatum (Lam) and monitoring the antibacterial activities. Arch Appl Sci Res 2012;4:2098-104.
11. Roshan Adhikari, Naveen Kumar HN, Shruthi SD. A review on medicinal importance of Basella alba L. Int J Pharm Sci Drug Res 2012;4:110-4.
12. Olowokudejo JD, Kadiri AB, Travih VA. An Ethanobotanical survey of herbal markets and medicinal plants in lagos state of Nigeria. J Ethanobotanical Leaflets 2005;12:851-65.
13. Bamidele O, Akinnuga AM, Olorunfemi JO, Odetola OA, Oparaji CK, Ezeigbo N. Effects of aqueous extract of Basella alba leaves on hematological and biochemical parameters in albino rats. Afr J Biotech 2010;9:6952-5.
14. Premakumari KB, Siddiqua Ayesha, Banu Shanaz, Josephine J, Jenita Leno, Raj Binc. Comparative antimicrobial studies of methanolic extract of Muntingia calabura, Basella alba and Basella rubra leaves. J Pharmacogn Phytochem 2010;2:246-8.
15. Krishna Chaitaniya B. Anti-inflammatory activity of basella alba linn in albino rats. J Appl Pharm Sci 2012;2:87.
16. Azad AK, Wan Azizi WS, Babar ZM, Zubair Khalid Labu, Zabin S. An Overview on phytochemical, Anti-inflammatory, Anti-bacterial activity of Basella alba Leaves extract. Middle-East J Sci Res 2013;14:650-5.
17. Inbathamizh L, Mekalai Ponnu T, Jancy Mary E. In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. J Pharm Res 2013;6:32-8.
18. Harborne JB. Phytochemical methods. A guide to modern techniques of plant analysis; 1992;279.
19. Isabel Ferreira CFR, Paula Baptista, Miguel Vilas-Boas, Lillian Barros. Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations. Food Chem 2007;100:1511-6.
20. Oyaizu M. Studies on products of browning reactions: antioxidative activities of browning reaction prepared from glucosamine. Japanese J Nut 1986;44:307-15.
21. Ergon Stahl. Thin Layer Chromatography. 2nd ed. A laboratory handbook; 1969;687.
22. Himaja M, Das Poppy, Karigar Asif. Green-Technique solvent free synthesis and its advantages. Int J Res Ayurvedha Pharm 2011;2:1079-86.
23. Hajar Zamani, Ali Moradshahi. Synthesis and coating of nanosilver by vanillic acid and its effects on Dunaliella salina Teod. Mol Cell Biol Res Commun 2013;2:47-55.
24. Dejan Orcic Z, Neda Mimica-Dukic M, Marina Franciskovic M, Slobodan Petrovic S, Emilija Jovin D. Antioxidant activity relationship of phenolic compounds in Hypericum perforatunm L. Chem Cent J 2011;5:34.