A CHEAP METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS USING UV SPECTROPHOTOMETRY


Vijay Kumar Dandu, Swetha Parupugalla, Chandra Mohan Thota, Ashok Kumar Akuthota

Abstract


Objective: To develop a cheap and a rapid UV spectrophotometric method for the quantitative estimation of Acamprosate calcium (333mg) in tablets and validate as per ICH guidelines.

Methods: The optimized method uses 100% distilled water as a solvent for the estimation of assay of Acamprosate calcium in tablets at a detection wavelength of 208 nm.

Results: The developed method resulted in Acamprosate calcium exhibiting linearity in the range 30-90μg/ml. The precision is exemplified by relative standard deviation of 0.425%. Percentage Mean recovery was found to be in the range of 98‐102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) were found to be 66mg/ml, and 200 mg/ml respectively.

Conclusion: A cheap and a rapid UV spectrophotometric method was developed and validated for the quantitative estimation of Acamprosate calcium in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.

 


Keywords


UV, Acamprosate calcium, Method development, Validation.

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About this article

Title

A CHEAP METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS USING UV SPECTROPHOTOMETRY

Keywords

UV, Acamprosate calcium, Method development, Validation.

Date

01-09-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 8, 2014 Page: 371-374

Online ISSN

0975-1491

Statistics

78 Views | 27 Downloads

Authors & Affiliations

Vijay Kumar Dandu
Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal), Hyderabad 501511, India
India

Swetha Parupugalla
Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal), Hyderabad 501511, India
India

Chandra Mohan Thota
Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal), Hyderabad 501511, India
India

Ashok Kumar Akuthota
Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal), Hyderabad 501511, India
India


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