ANALYTICAL METHOD BY HPLC-DAD ALLOWS QUANTIFICATION OF QUERCETIN MARKER IN STANDARDIZED EXTRACT OF ANADENANTHERA COLUBRINA VAR. CEBIL


Valmir Gomes De Souza, FabrÍcio Havy Dantas De Andrade, Fabio Santos De Souza, Rui Oliveira Macedo

Abstract


Objective: The Anadenanthera colubrina (Vell.) Brennan var. cebil is a medicinal plant that has been used for the treatment of many diseases in the northeastern region of Brazil. This plant contains secondary metabolites such as quercetin, a flavonoid that is known by its antioxidant and anti-inflammatory effects. The aim of this work is to propose the validation of an analytical method using high-performance liquid chromatography with diode array detector (HPLC-DAD) for the quantification of quercetin and standardization of the hydroalcoholic extract (HAE) of A. colubrina.

Methods: The A. colubrina extracts were prepared by the maceration process with powdered leaves at 20% weight: volume (w/v) and a hydroalcoholic solution at 50% volume: volume (v/v) for 120 h at room temperature. After pretreatment of the hydroalcoholic extract, the quercetin marker was used for quantification and proceeded to the evaluation of validation parameters for the method using HPLC-DAD.

Results: The analytical method proved to be specific. Linear over the range 1.4–26.6 µg/ml, regression analysis showed a good correlation coefficient (R2= 0.999); the limit of detection (LOD) and the limit of quantification (LOQ) were 0.27 and 0.81 μg/ml respectively. The relative standard deviation (RSD) did not exceed 2.5% for precision. The proposed method was validated with an average recovery of 92.5–97.5%.

Conclusion: The method was validated using HPLC-DAD, allowing the quantification of quercetin in the standardisation process of extracts and quality control of the herbal drug containing A. colubrina Phyto complex.


Keywords


Validation, Analytical, Method, Quercetin, Flavonoid, Anadenanthera colubrina

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About this article

Title

ANALYTICAL METHOD BY HPLC-DAD ALLOWS QUANTIFICATION OF QUERCETIN MARKER IN STANDARDIZED EXTRACT OF ANADENANTHERA COLUBRINA VAR. CEBIL

Keywords

Validation, Analytical, Method, Quercetin, Flavonoid, Anadenanthera colubrina

DOI

10.22159/ijpps.2017v9i8.16468

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 8, 2017 Page: 47-55

Online ISSN

0975-1491

Statistics

161 Views | 23 Downloads

Authors & Affiliations

Valmir Gomes De Souza
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazi
Brazil

FabrÍcio Havy Dantas De Andrade
Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife-PE, Brazil
Brazil

Fabio Santos De Souza
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazil
Brazil

Rui Oliveira Macedo
Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife-PE, Brazil
Brazil


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