PREPARATION, CHARACTERIZATION AND EVALUATION OF POLY (LACTIDE –CO –GLYCOLIDE) MICROSPHERES FOR THE CONTROLLED RELEASE OF ZIDOVUDINE


Seema Kohli, Abhisek Pal, Suchit Jain

Abstract


Objective: The purpose of this research work was to develop and evaluate microspheres appropriate for controlled release of zidovudine (AZT).

Methods: The AZT loaded polylactide-co-glycolide (PLGA) microspheres were prepared by W/O/O double emulsion solvent diffusion method. Compatibility of drug and polymer was studied by Fourier-transform infrared spectroscopy (FTIR). The influence of formulation factors (drug: polymer ratio, stirring speed, the concentration of surfactant) on particle size encapsulation efficiency and in vitro release characteristics of the microspheres was investigated. Release kinetics was studied and stability study was performed as per ICH guidelines.

Results: Scanning electron microscopy (SEM) images show good reproducibility of microspheres from different batches. The average particle size was in the range of 216-306 μm. The drug-loaded microspheres showed 74.42±5.08% entrapment efficiency. The cumulative percentage released in phosphate Buffer solution (PBS) buffer was found to be 55.32±5.89 to 74.42±5.08 %. The highest regressions (0.981) were obtained for zero order kinetics followed by Higuchi (0.968) and first order (0.803).

Conclusion: Microsphere prepared by double emulsion solvent diffusion method was investigated and the results revealed that 216-306 μm microsphere was successfully encapsulated in a polymer. FT-IR analysis, entrapment efficiency and SEM Studies revealed the good reproducibility from batch to batch. The microspheres were of an appropriate size and suitable for oral administration. Thus the current investigation show promising results of PLGA microspheres as a matrix for drug delivery and merit for In vivo studies for scale up the technology.


Keywords


Zidovudine, PLGA, Microspheres, W/O/O double emulsion, Higuchi model, Diffusion-Controlled Mechanism

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About this article

Title

PREPARATION, CHARACTERIZATION AND EVALUATION OF POLY (LACTIDE –CO –GLYCOLIDE) MICROSPHERES FOR THE CONTROLLED RELEASE OF ZIDOVUDINE

Keywords

Zidovudine, PLGA, Microspheres, W/O/O double emulsion, Higuchi model, Diffusion-Controlled Mechanism

DOI

10.22159/ijpps.2017v9i12.18377

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 12, 2017 Page: 70-77

Online ISSN

0975-1491

Statistics

25 Views | 7 Downloads

Authors & Affiliations

Seema Kohli
Department of Pharmacy, Kalaniketan Polytechnic, Jabalpur M.P.
India

Abhisek Pal
School of Pharmaceutical Sciences, Shiksha ‘O’Anusandhan University, Bhubaneswar Orissa

Suchit Jain
School of Pharmaceutical Sciences, Shiksha ‘O’Anusandhan University, Bhubaneswar Orissa


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