ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF METHANOLIC EXTRACT OF BAUHINIA RACEMOSA
Objective: To evaluate antimicrobial and in vitro [s1]Â antioxidant activities of methanolic extract of Bauhinia racemosa leaf.
Methods: The antimicrobial activity was determined by the agar-well diffusion method. The antioxidant potential was evaluated by using various in vitro [s2]Â methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), phospho molybdenum reduction assay and ferric (Fe 3+) reducing power assays.
Results: The maximum inhibition zone (26 mm) was found against Proteus mirabilis while lowest inhibition zone (20 mm) was observed against Acinetobacter baumannii.
The DPPH and ABTS were significantly inhibited by methanolic extract of Bauhinia racemosa leaf with IC50 of 9 Î¼g/ml and 550Î¼g/ml respectively, whereas methanolic extract of leaves showed good antioxidant potential using ferric reducing power assay (1.896[s3]Â and phosphomolybdate assay (108.12 mg AAE/g) methods.
Conclusion: The results of present study showed that Bauhinia racemosa leaf possesses high potential antimicrobial and antioxidant activity and could be a potential source of natural antioxidant that could have great importance as therapeutic agents in preventing oxidative stress-related degenerative diseases.Keywords: Bauhinia racemosa, Antimicrobial, In vitro [s4]Â antioxidant, DPPH, ABTS, FRPA.
Â [s3]IC50 or what?
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