STRUCTURE CHARACTERIZATION AND EVALUATION POTENTIAL OF ANTIMICROBIAL EXTRACTS FROM PHELLINUS LINTEUS AGAINST SKIN INFECTIOUS PATHOGENS, STAPHYLOCOCCUS EPIDERMIDIS ATCC12228 AND PROPIONIBACTERIUM ACNES DMST14916


Surachai Techaoei, Pattaranut Eakwaropas, Khemjira Jarmkom, Warachate Khobjai

Abstract


Objective: The objective of this study was to investigate the antimicrobial activity of Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis ATCC12228 and Propionibacterium acnes DMST 14916.

Methods: Fungal fruiting bodies were extracted with 95% ethanol and ethyl acetate, and then, vaporized. The antimicrobial activities were determined by the disc diffusion method against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 skin infectious pathogens. A minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) for those crude extracts were determined. Finally, the chemical profile of crude extract was determined by using thin layer chromatography and GC-MS.

Results: The result demonstrated that the ethanolic extraction had more active fractions with an MIC of 0.5 mg/ml against the growth of Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 and also showed a minimum inhibitory concentration (MBC) at a concentration of 1.0 mg/ml, while ethyl acetate-based solvents failed to develop on TLC according to Retention factor (Rf) values of 0.71-0.76. The GC-MS was applied to investigate the chemical profile of crude extract of Phellinus linteus, revealing a component of hexadecanoic acid and 9, 12-octadecadienoic acid.

Conclusion: Phellinus linteus fruiting body extracts have great potential as antimicrobial compounds against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228. Thus, they can be used in the treatment of infectious diseases caused by bacterial pathogens. 


Keywords


Antimicrobial activity, Phellinus linteus, Staphylococcus epidermidis, Propionibacterium acnes, MIC, MBC

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About this article

Title

STRUCTURE CHARACTERIZATION AND EVALUATION POTENTIAL OF ANTIMICROBIAL EXTRACTS FROM PHELLINUS LINTEUS AGAINST SKIN INFECTIOUS PATHOGENS, STAPHYLOCOCCUS EPIDERMIDIS ATCC12228 AND PROPIONIBACTERIUM ACNES DMST14916

Keywords

Antimicrobial activity, Phellinus linteus, Staphylococcus epidermidis, Propionibacterium acnes, MIC, MBC

DOI

10.22159/ijpps.2017v9i12.19032

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 12, 2017 Page: 78-81

Online ISSN

0975-1491

Statistics

55 Views | 14 Downloads

Authors & Affiliations

Surachai Techaoei
Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130 Thailand
Thailand

Pattaranut Eakwaropas
Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130 Thailand

Khemjira Jarmkom
Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130 Thailand

Warachate Khobjai
Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130 Thailand


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