ALPHA AMYLASE INHIBITORY ACTIVITY OF DIFFERENT EXTRACT OF BARK OF ALBIZIA LEBBECK (L.) BENTH
Objective: To evaluate the alpha amylase inhibitory activity of different extract of bark of Albizia lebbeck (L.) Benth.
Methods: Bark of A. lebbeck was collected and air dried. Extraction of flavonoid, alkaloid and steroid carried out using standard methods. Extracts in different polar and non polar solvents were extracted using soxhlet method. Filtered extracts were subsequently tested for alpha amylase inhibitory activity using the chromogenic dinitrosalicylic acid (DNSA) method and starch iodine method. Experiments were performed in triplicates for three different sets and meanÂ±standard error of the mean was used for calculation. Graphpad prism 5 software was used for one way analysis of variance (ANOVA), linear regression and statistical difference analysis. IC50 values were also calculated.
Results: We found that the free flavonoid extract had higher 43.50Â±0.17% to 73.43Â±0.08% inhibition (at a concentration 0.5-1.5 mg/ml) with an IC50 value of 0.6653 mg/ml. At the same concentration methanol, water and bound flavonoid extracts also showed good inhibitory activity i.e. 28.63Â±0.15% to 37.50Â±0.20%, 26.67Â±0.12% to 32.07Â±0.17% and 26.70Â±0.15 to 32.67Â±0.12% with an IC50 value of 7.3621 mg/ml, 22.2844 mg/ml and 41.6869 mg/ml respectively.
Conclusion: The prepared free flavonoid, bound flavonoid, water and methanol bark extracts of Albizia lebbeck showed the significant alpha amylase inhibitory activity. Further works is required to identify the bioactive compound responsible for inhibition of alpha amylse enzyme.
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