COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF SERIAL EXTRACTS FROM LEAVES AND FRUIT OF AEGLE MARMELOS AND CARICA PAPAYA
Objective: The present study was carried out to evaluate the in vitro antimicrobial activity of water, methanolic and pet ether extracts from leaves and fruit of A. marmelos and C. papaya.
Methods: Crude extract of leaves and fruit of Aegle marmelos and Carica papaya were prepared for series of polar solvents by hot extraction method in soxhlet then the extracts were first screened for its antimicrobial activity by â€œDisc Diffusion Assayâ€ against medically important bacteria, plant pathogen and fungi. Fractions showing activities were then used to determine MIC (minimum inhibitory concentration) and MBC/MFC (minimum bactericidal and fungicidal concentration) by broth dilution and total activity were also calculated.
Result: Aegle marmelos shows comparatively significant antimicrobial activity than Carica papaya. However antimicrobial activity was found both solvent and organism dependent. Almost all the extracts of Aegle marmelos show an inhibitory effect against most of the test organism whereas the test extracts Carica papaya show an inhibitory effect against the very few test organisms. Lowest MIC values 0.0195 mg/ml were recorded against Roultella planticola and Klebsiella pneumonae indicate the significant antimicrobial potential of the test extracts. The high value of TA was recorded against Roultella planticola in both the plants. Data were analyzed by one way ANNOVA and values were considered significant at P<0.05
Conclusion: The MIC and MBC/MFC values of Aegle marmelos showed its broad antimicrobial potential and is promising in the development of phytomedicine for antimicrobial properties then Carica papaya.
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