MYRICETIN ISOLATED FROM TURBINARIA ORNATA AMELIORATES ROTENONE INDUCED PARKINSONISM IN DROSOPHILA MELANOGASTER


Vijayraja Dhanraj, Tamilarasan Manivasagam, Jeyaprakash Karuppaiah

Abstract


Objective: Parkinson’s disease (PD) is a neurodegenerative disorder which affects the elderly population. Free radicals overproduction, oxidative stress, apoptosis, inflammation and abnormalities in mitochondria are critical mediators of the neuronal degeneration. In the present study neuroprotective activity of myricetin, a flavonoid isolated from brown seaweed Turbinaria ornata have been investigated in rotenone induced experimental PD models of Drosophila melanogaster.

Methods: Male fruit flies (Drosophila melanogaster) were fed with an effective dose of 0.1% myricetin three hours before to the treatment with 500 µM of Rotenone (LD 50) for seven days and on 8th day through behavioral analysis the neuroprotective effect of myricetin was investigated for motor coordination in fruit flies. Lipid peroxidation was analyzed by estimating the levels of TBARS. Oxidative stress was determined by estimating the activities of enzymatic antioxidants superoxide dismutase, catalase, and glutathione peroxidase along with the level of reduced glutathione. Dopamine level was estimated in HPLC column detected at 280 nm with UV detectors and degree of apoptosis was studied apoptotic marker Bcl-2, Bax, caspases-3 and 9, cytochrome c and β-actin expressions in the whole body homogenate of fruit flies of experimental groups homogenized in 500μL of 0.1 M phosphate buffers (ice cold, pH, 7.4) containing 1 mmol EDTA.

Results: Myricetin maintains the positive behavioral patterns against motor impairments due to the rotenone toxicity, it creates a balance in oxidant and antioxidant status, reduces the oxidative stress and inhibits apoptosis to retard neurodegeneration and maintains the dopamine level with a significant (p<0.05) difference compared to the rotenone treated group.

Conclusion: The flavonoid myricetin by reducing the oxidative stress, maintaining the enzymatic antioxidants status and by inhibiting apoptosis prevents the degeneration of dopaminergic neurons. The dopaminergic neurons prevention reduces the depletion of dopamine and thereby promotes the muscular coordination and psychological well being of fruit flies of experimental group. Further in depth molecular level studies are in need to explore the preventive mechanisms of myricetin in Parkinson’s disease.


Keywords


Parkinson’s disease, Myricetin, Rotenone, Climbing assay, Apoptosis

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References


Khadira Sereen A, Vijayalakshmi K, Priya Nagappan, Shinu Balima. Effect of sesamol in association with folic acid on 6-OHDA induced Parkinsonian animals-biochemical, neurochemical and histopathological evidence. Asian J Pharm Clin Res 2017;10:46-50.

Vera Dias, Eunsung J, Maral Mouradian M. The role of oxidative stress in parkinson’s disease. J Parkinson's Dis 2013;3:461-91.

Suryakanta Pany, Abhisek Pal, Pratap Kumar Sahu. Neuroprotective effect of quercetin in neurotoxicity induced rats: role of neuroinflammation in neurodegeneration. Asian J Pharm Clin Res 2014;7:152-6.

Uchihara T, Giasson BI. Propagation of alpha-synuclein pathology: hypotheses discoveries, and yet unresolved questions from experimental and human brain studies. Acta Neuropathol 2016;131:49-73.

Butterfield DA, Perluigi M, Reed T, Muharib T, Hughes CP, Robinson RA, et al. redox proteomics in selected neurodegenerative disorders: from its infancy to future applications. Antioxid Redox Signal 2012;17:1610-55.

Mariya V, Vinoth S. Biomedical and pharmacological significance of marine macro algae-review. Indian J Mar Sci 2013;42:527-37.

Vijayraja D, Jeyaprakash K. Phytochemical analysis, in vitro antioxidant and anti-hemolysis activity of Turbinaria ornata (turner) J. Agardh Int J Adv Res Sci Eng Technol 2015;12:45-9.

Vijayraja D, Jeyaprakash K. Phytochemical analysis, in vitro DPPH free radical scavenging, anti-hemolysis and anti-inflammatory activities of Turbinaria ornata (Turner) J. Agardh World J Pharm Pharm 2016;5:1231-8.

Crozier A, Lean MEJ, McDonald MS, Black C. Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce and celery. J Agric Food Chem 1997;45:590-5.

Das S, Ganapaty S. Phytochemical evaluation of roots of polygonum viscosum buch-ham. Indian J Pharm Sci 2015;77:352-6.

Bilen J, Bonini NM. Drosophila as a model for human neurodegenerative disease. Annu Rev Genet 2005;39:153-71.

Hosamani R, Ramesh SR, Muralidhara. Attenuation of rotenone-induced mitochondrial oxidative damage and neurotoxicty in Drosophila melanogaster supplemented with creatine. Neurochem Res 2010;35:1402-12.

Jeibmann A, Paulus W. Drosophila melanogaster as a model organism of brain diseases. Int J Mol Sci 2009;10:407-40.

Jimenez-Del-Rio M, Daza-Restrepo A, Velez-Pardo C. The cannabinoid CP55, 940 prolongs survival and improves locomotor activity in Drosophila melanogaster against paraquat: implications in Parkinson’s disease. Neurosci Res 2008;61:404-11.

Anandhan A, Tamilselvam K, Vijayraja D. Resveratrol attenuates oxidative stress and improves behaviour in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) challenged mice. Ann Neurosci 2010;17:113-9.

Hirth F. Drosophila melanogaster in the study of human neurodegeneration. CNS Neurol Disord Drug Targets 2010;9:504-23.

Liu Z, Wang X, Yu Y, Li X, Wang T, Jiang H, et al. A drosophila model for LRRK2-linked parkinsonism. Proc Natl Acad Sci USA 2008;105:2693-8.

Dalpiaz A, Filosa R, de Caprariis P, Conte G, Bortolotti F, Biondi C, et al. Molecular mechanism involved in the transport of a prodrug dopamine glycosyl conjugate. Int J Pharm 2007;336:133-9.

Utley HC, Bernheim F, Hochslein P. Effect of sulfhydryl reagent on peroxidation in microsome. Arch Biochem Biophys 1967;118:29-32.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.

Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44:276-87.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.

Jollow DJ, Mitchell JR, Zampagloine N, Gillete JR. Bromobenzene-induced liver necrosis: protective role of glutathione and evidence for 3, 4 bromobenzeneoxide as the hepatotoxic intermediate. Pharmacology 1974;11:151-69.

Mohandas J, Marshall JJ, Duggin GG, Horvath JS, Tiller DJ. Differential distribution of glutathione and glutathione related enzymes in rabbit kidneys: Possible implication in analgesic neuropathy. Cancer Res 1984;44:5086-91.

Tamilselvam K, Braidy N, Manivasagam T, Essa MM, Prasad NR, Karthikeyan S, et al. Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease. Oxid Med Cell Longev 2013:102741. Doi:10.1155/2013/102741.

Joao Massano, Kailash P Bhatia. Clinical approach to Parkinson's disease: features, diagnosis, and principles of management. Cold Spring Harbor Perspect Med 2012;2:1-15.

Manjunath MJ, Muralidhara. Standardized extract of Withania somnifera (Ashwagandha) markedly offsets rotenone-induced locomotor deficits, oxidative impairments and neurotoxicity in Drosophila melanogaster. J Food Sci Technol 2015;52:1971-81.

Nataraj J, Manivasagam T, Thenmozhi AJ, Essa MM. Lutein protects dopaminergic neurons against MPTP-induced apoptotic death and motor dysfunction by ameliorating mitochondrial disruption and oxidative stress. Nutr Neurosci 2016;19:237-46.




About this article

Title

MYRICETIN ISOLATED FROM TURBINARIA ORNATA AMELIORATES ROTENONE INDUCED PARKINSONISM IN DROSOPHILA MELANOGASTER

Keywords

Parkinson’s disease, Myricetin, Rotenone, Climbing assay, Apoptosis

DOI

10.22159/ijpps.2017v9i11.19931

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 39-44

Online ISSN

0975-1491

Statistics

43 Views | 12 Downloads

Authors & Affiliations

Vijayraja Dhanraj
Research and Development Centre, Bharathiar University, Coimbatore-641046, Tamilnadu, India
India

Tamilarasan Manivasagam
Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram-608002, Tamil Nadu, India.

Jeyaprakash Karuppaiah
PG and Research Department of Biochemistry, Rajah Serfoji Government College, Thanjavur-613005, India


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