SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY

Authors

  • S. Narendhran Department of Biotechnology, Karpagam University, Coimbatore 64102
  • P. Rajiv Department of Biotechnology, Karpagam University, Coimbatore 64102
  • P. Vanathi Department of Biotechnology, Karpagam University, Coimbatore 64102
  • Rajeshwari Sivaraj Department of Biotechnology, Karpagam University, Coimbatore 641021

Keywords:

Anticancer, Antioxidant, Bioactive compound, Streptomyces cavouresis KU-V39, FT-IR and GC-MS

Abstract

Objective: To assess the antioxidant and anticancer activity of crude ethyl acetate extract from Streptomyces cavouresis KU-V39 isolated from vermicompost.

Methods: To determine its antioxidant activity by total phenol and flavonoid content, Hydrogen peroxide radical scavenging activity and Ferrous reducing power assay. To determine in vitro cytotoxicity, various concentrations of extract was tested on HeLa cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectroscopy (GC-MS) have been carried out to investigate the bioactive compound of crude extract.

Results: The Phenol and flavonoid content of crude extract was found to be 20.24 and 26.60 mg /g metabolite. Isolate KU-V39 exhibited H2O2 scavenging activity at IC50 value is 42.35 ± 0.75 μg/ml. The ferrous reducing power assay has higher absorbance value which indicates the high antioxidant capacity of the extracts. The analysis of FT-IR spectroscopy indicate presents of Phenol, carboxylic acid, alkanes, nitro and aliphatic amines functional groups. The GC – MS analysis revealed that presence of Pentanoic acid, L-Proline, Lysine, Erucic acid, isosteviol, Pentadecanoic acid, Phthalic acid, Hexadecanoic acid, Octadecenoic acid, Dichloroacetic acid, Cyclohexanecarboxylic acid and Pyrrolo pyrazine derivative.

Conclusion: This spectral study clearly proved that the vermicompost derived Streptomyces with extra cellular metabolism showed promising antioxidant and anticancer activity.

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Published

01-07-2014

How to Cite

Narendhran, S., P. Rajiv, P. Vanathi, and R. Sivaraj. “SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 319-22, https://innovareacademics.in/journals/index.php/ijpps/article/view/2009.

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