DISCRIMINANT ANALYSIS OF PURIFICATION ON TURMERIC (CURCUMA LONGA LINN) SAMPLES BASED ON PHYTO-PHARMACOGNOSTICAL AND MULTIVARIATE CHEMOMETRIC TECHNIQUE


Anuj Kumar, Akanksha Thakur, Jayanta Kumar Maji, Prashant Bedarkar

Abstract


Objective: To establish an acceptable identification system of various purification effects in context classical based different media on turmeric samples and relates its altering pattern in favor of phyto-pharmacognostical, image processing and multivariate chemometric analysis.

Methods: Authenticated turmeric samples purified through different processes by using different media such as cow’s urine, panchapllava (five different plants tender leaves), the inflorescence of alambusha (Sphaeranthus indicus, Linn) decoction, water and buttermilk. Resultant samples dried, pulverized and undertaken powder microscopy, image processing, physicochemical and chromatographic fingerprinting (HPTLC). The multivariate chemometric analysis, principal component analysis (PCA) analyzed with help of Unscrambler and image processing in Matlab software.

Results: The addition of characters of medias drug with turmeric powders like the crystal of gomutra, pollen grain and starch grain of Alambusha, epidermis, fibre, the crystal of panchapllava. Identify different perceivable colors in variously processed turmeric by analyzing the Lab color space through the Image segmentation. PC1 and PC2 explained (90 + 9) % total variance in score plot of respective purify turmeric samples shown clear grouping in relation to the physicochemical constant. Quantification of curcumin in various treated turmeric samples displayed variation due to additive effect in high-performance thin layer chromatographic profile.

Conclusion: This study proved that purification in ayurveda not only refers to the elimination of toxins and unwanted particles but also the transformation in the properties in the primary substance rendering it safe as well as many desired qualities are imbibed in it.


Keywords


Curcumin, Discrimination, HPTLC, Image Processing, Powder-Microscopy, Purification, Turmeric

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References


Harish K Handral, Shrishail Duggi, Ravichandra Handral, Tulsianand G, Shruthi S D. Turmeric: nature’s precious medicine. Asian J Pharm Clin Res 2013;6:10-6.

Khare CP. Indian medicinal plants. 1st ed. Spinger Science+buiseness media, LLC, New-York, USA; 2007.

Roman Adhikari, Jyothi Y, Deepika Bora, Vamsee Veena A. Combined effect of aqueous extract of curcuma longa linn. with metformin in diabetes induced neuroptahic pain in rats. Asian J Pharm Clin Res 2015;8:166-70.

Govind Das. Vatavyadhichikitsa-26/447 in bhaisajyaratnavali. 15th edi. Varanasi Chaukhamba Sanskrita samsthana; 2001. p. 402.

Sudipta Roy, Rabinarayan Acharya, Vinay J Shukla. Comparative physicochemical profile of Gunja (Abrus precatorius Linn.) seeds processed through water and nimbu swarasa (lemon juice). AYU 2013;34:411-8.

Swarnendu Mitra, VJ Shukla, Rabinarayan Acharya. Effect of sodhan on (processing) on Kupeelu (Strychnos nuxvomica Linn.) with special reference to strychnine and brucine content. AYU 2011;32:402-6.

Vı́zhányó T, Felföldi J. Enhancing color differences in images of diseased mushrooms. Computers Electronics Agriculture 2000;26:187-98.

Jayanta Kumar Maji, Sohim VJ Shukla. Application of multivariate curve resolution alternating least square (MCR-ALS) to the study of trikarshika formulation. Int J Pharm Pharm Sci 2017;8:238-43.

Jayanta Kumar Maji, Shikha Sharma, VJ Shukla. Image processing and ultra-violet and visible reflectance spectroscopy combined with chemometric for discrimination as well as authentification powder and extract with anti-diabetic polyherbal formulation. Int J Pharma Sci Res 2016;8:325-34.

Siddhinanadana Mishra. Harmekhala. 1st ed. Chaukhambha Publication, Varansi; 2013. p. 166.

Ramtejpandeyana. Bhavaprakash nighantoo. 3rd edition. Varansi: Chaukhamba Sanskrita Pratisthana. 2003. p. 304.

Thomas Lijio, P Rajeev. Turmeric-extension pamphlet. published by Kozhikode ICAR; 2015. p. 9-10.

Govind Das. Vatavyadhichikitsa-26/447 in Bhaisajyaratnavali. 15th edition. Varansi: Chaukhamba Sanskritasamsthana, 2001. p.-404.

Triphati Jagdishprasada. Vatavyadhichikitsa 22/287 in Chakradatta of Chakarpanidatta. 5th edition. Varansi: Chaukhamba Sanskrit series office. 1983. p.211.

Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 42nd ed. Nirali Prakashan, Pune, 2008. Pg-6.3.

Quality standard of Indian Medicinal plants, published by–Indian Council of Medical Research, 2003. p. 102-12.

Anonymous. Ayurvedic Pharmacopoeia of India. Part-I e-Book Appendix Volume(s)–I to IV, New Delhi: Govt. of India: Ministry of Health and Family Welfare, Department of Indian Systems of Medicine and Homeopathy; 2001.

Ashraf Kamran. Validated HPTLC analysis method for quantification of variability in content of curcumin in curcuma longa L. (turmeric) collected from different geographical region of India. Asian Pac J Trop Biomed 2012;2:584-8.

Anonymous. Ayurvedic Pharmacopoeia of India. Part-I e-Book Appendix Volume(s)–I to IV, New Delhi: Govt. of India: Ministry of Health and Family Welfare, Department of Indian Systems of Medicine and Homeopathy; 2001.

Ibidem The Ayurvedic Pharmacopoeia of India. Part-I e-Book Appendix Volume(s)–I to IV, Appendix 2.2.3; 2008.

Ibidem The Ayurvedic Pharmacopoeia of India. Part-I e-Book Appendix Volume(s)–I to IV, Appendix 2.2.4; 2008.

Ibidem The Ayurvedic Pharmacopoeia of India, Part-I e-Book Appendix Volume(s)–I to IV, Appendix 2.2.5; 2008.

Armstrong J Scott. International journal of forecasting (forthcoming). Illusions Regression Anal 2012;28:689.

Ajay Kumar. Antioxidant efficacy and curcumin content of turmeric (Curcuma longa Linn.) flower. Int J Curr Pharm Res 2016;8:112-4.

Chan MM. Inhibition of tumor necrosis factor by curcumin a phytochemical. Biochem Pharmacol 1995;45:1551-6.




About this article

Title

DISCRIMINANT ANALYSIS OF PURIFICATION ON TURMERIC (CURCUMA LONGA LINN) SAMPLES BASED ON PHYTO-PHARMACOGNOSTICAL AND MULTIVARIATE CHEMOMETRIC TECHNIQUE

Keywords

Curcumin, Discrimination, HPTLC, Image Processing, Powder-Microscopy, Purification, Turmeric

DOI

10.22159/ijpps.2017v9i9.20478

Date

13-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 9, 2017 Page: 156-163

Online ISSN

0975-1491

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123 Views | 47 Downloads

Authors & Affiliations

Anuj Kumar
M. Pharma (Ayu.), Department of RS & BK, Gujarat Ayurveda University, Jamnagar, Gujarat.
India

Akanksha Thakur
M. Pharma (Ayu.), Department of Pharmacognosy, Gujarat Ayurveda University, Jamnagar, Gujarat.
India

Jayanta Kumar Maji
Phd scholar (Ayu), Department of Pharmaceutical Chemistry, Gujarat Ayurveda University, Jamnagar, Gujarat.
India

Prashant Bedarkar
Ass. Prof., Department of RS & BK, IPGT & RA, Gujarat Ayurveda University, Jamnagar, Gujarat.


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