POTENTIAL ANTI-TUMOR AND ANTI-INFLAMMATORY ACTIVITY OF SIX MISTLETOE PLANTS IN THE FAMILY VISCACEAE PRESENT IN WESTERN GHATS, INDIA


Ramadasan Kuttan, Sivamurthy R. G., Girija Kuttan

Abstract


ABSTRACT

Objectives

To find out the cytotoxicity, anti-tumor and anti-inflammatory activities of six species of plants belongs to Viscaeceae family available in Western Ghats (India).

Methods

In vitro cytotoxicity of Viscum extracts was studied by trypan blue exclusion method and MTT assay using various cell lines. Anti-tumor activity was determined using Ehrlich ascites carcinoma (EAC) and Dalton’s lymphoma ascites (DLA) cells in mice. Anti-inflammatory activities of Viscum extracts were studied using carrageenan and dextran induced mouse paw edema models in mice.

Results

All six Viscaeceae plant extracts studied were cytotoxic towards transformed cell lines like DLA and EAC as well as to MCF-7, MDA-MB-231 and SKBR3 cell lines. V.orientale, V.nepalense and V.ramosissimum, V.trilobatum were cytotoxic towards normal cells while V.angulatum and V.capitellatum were found to be nontoxic. Excepting V.angulatum all the other species selected here showed toxicity to animals. Administration of nontoxic concentration of extracts of Viscaeceae plants significantly (P<0.001) increased the lifespan of ascites tumor bearing animals and reduced DLA cells induced solid tumor development. All these plants except V.capitallatum and V.trilobatum showed significant (P<0.001) anti-inflammatory activity against carrageenan and dextran models and reduced pro-inflammatory cytokine levels.

Conclusion

Out of six species studied four species of Viscum species studied were cytotoxic to tumour cells and inhibited tumour development. Of the six species studied V. angulatum was non-toxic to animals and showed maximum efficiency as an antitumour agent. These plants showed significant anti-inflammatory activity and reduced inflammatory markers.


Keywords


Semiparasitase plants – Viscum species - Tumour reducing activity, Anti-inflammatory activity, Pro-inflammatory cytokines.

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About this article

Title

POTENTIAL ANTI-TUMOR AND ANTI-INFLAMMATORY ACTIVITY OF SIX MISTLETOE PLANTS IN THE FAMILY VISCACEAE PRESENT IN WESTERN GHATS, INDIA

Keywords

Semiparasitase plants – Viscum species - Tumour reducing activity, Anti-inflammatory activity, Pro-inflammatory cytokines.

DOI

10.22159/ijpps.2017v9i11.20541

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 57-64

Online ISSN

0975-1491

Statistics

52 Views | 46 Downloads

Authors & Affiliations

Ramadasan Kuttan
Research Director, Amala Cancer Research Centre, Amala Nagar P.O, Thrissur-680 555, Kerala, India
India

Sivamurthy R. G.
Dr. R.G.Sivamurthy, Retd Professor, Department of Botany, University of Mysore, Mysore
India

Girija Kuttan
Dr. Girija Kuttan, Professor, Department of Immunology, Amala Cancer Research Centre, Amala Nagar P.O, Thrissur-680 555, Kerala, India
India


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