EVALUATION OF ANTIDIABETIC, HYPOLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF POLYHERBAL FORMULATION IN STREPTOZOTOCIN-NICOTINAMIDE INDUCED DIABETES IN RATS
Objective: To evaluate antidiabetic, hypolipidemic and antioxidant activity of polyherbalformulation (PHF) aqueous extract in streptozotocin-nicotinamide induced diabetes in rats.
Methods: Fasting blood glucose, lipid profiles, serum insulin and glycosylate haemoglobin (HbA1C) were determined in normal and streptozotocin-nicotinamide induced diabetic rats after oral administration of the PHF for 45 d. Antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) levels were evaluated in kidney and liver tissue. Histopathological changes in diabetic rat vital organs were also observed after PHF treatment.
Results: Daily oral administration of PHF (200 and 400 mg/kg, b.w.) and metformin (5 mg/kg, b.w.) showed beneficial effects on blood glucose level (P<0.001) and hyperlipidaemia due to diabetes. The PHF treatment also enhances serum insulin level and body weight of diabetic rats as compared to diabetic control group. Furthermore, the PHF has favourable effects on histopathological studies, in streptozotocin-nicotinamide induced diabetes. Antioxidant enzymes and GSH levels were found to be significantly increased and levels of MDA were decreased in treated diabetic animals.
Conclusion: PHF possesses antidiabetic, hypolipidemic and antioxidant properties. PHF has also showed favourable effect on histopathological changes in streptozotocin-nicotinamide induced diabetic animals.
2. Navpreet K, Lalit K, Randhir S. Antidiabetic effect of new chromane isolated from Dillenia indica L. leaves in streptozotocin-induced diabetes rats. J Func Foods 2016;22:547-55.
3. Chin-Shiu H, Mei-Chin Y, Lan-Chi C. Antihyperglycemic and antioxidative potential of Psidium guajava fruit in streptozotocin-induced diabetic rats. Food Chem Toxicol 2011;49:2189-95.
4. Cadd WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. J Am Physical Ther Association 2008;88:1322-35.
5. Mamdouh MA, Abdel-Raheim MAM. Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol Part A 2003;135:539-47.
6. Subramaniam R, Aiyalu R, Manisenthi KTK. Antidiabetic, antihyperlipidemic and antioxidant potential of methanol extract of Tectona grandis flowers in streptozotocin-induced diabetic rats. Asian Pac J Trop Med 2011;4:624-31.
7. Bhatt M, Gahlot M, Juyal V, Singh A. Phytochemical investigation anti-diabetic activity of Adhatoda zeylanica. Asian J Pharm Clin Res 2011;4:27-30.
8. Rajesh R, Chitra K, Padmaa MP. Antihyperglycemic and antihyperlipidemic activity of aerial parts of Aerva lanata Linn Juss in streptozotocin-induced diabetic rats. Asian J Trop Biomed 2012;2:924-9.
9. Lawrence L, Richard B, Jyoti K, Susan H, Sharon C. Anti-diabetic and hypoglycaemic effects of Momordica charantia (bitter melon): a mini review. Br J Nutr 2009;102:1703â€“8.
10. Chhanda M, Kausik C, Mehuli GB, Debidas G. Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in streptozotocin-induced diabetic male albino rats. Afr J Trad CAM 2007;4:362-71.
11. Guy K, Jaekyung K, Klaus H, Yanyan C, Xiaozhuo C. Antidiabetes and anti-obesity activity of Lagerstroemia speciosa. eCAM 2007;4:401â€“7.
12. Anu C, Abbas AM, Sohil A, Raj KS. Indian herbs result in hypoglycaemic responses in streptozotocin-induced diabetic rats. Nutr Res 2007;27:161-8.
13. Guidance document on acute oral toxicity testing, series on testing and assessment No. 24, an organization for economic co-operation and development, OECD Environment, health and safety publications, Paris; 2001.
14. Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF. The changing faces of gluthione, a cellular pathogonist. Biochem Pharmacol 2003;66:1499-503.
15. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
16. Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.
17. Ohkawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
18. Malini P, Kanchana G, Rajadurai M. Antidiabetic efficacy of ellagic acid in streptozotocin-induced diabetes in albino wistar rats. Asian J Pharm Clin Res 2011;4:124-8.
19. Babu PS, Prabuseenivasan S, Ignacimuthu S. Cinnamaldehyde-A potential antidiabetic agent. Phytomedicine 2007;14:15-22.
20. Ravi K, Rajasekaran S, Subramanian S. Antihyperlipidemic effect of Eugenia jambolana seed kernel on streptozotocin-induced diabetes in rats. Food Chem Toxicol 2005;43:1433-9.
21. Tiwari BK, Pandey KB, Pandey KB, Abidi AB, Rizvi SI. Markers of oxidative stress during diabetes mellitus. J Biomark 2013:1-8. http://dx.doi.org/10.1155/2013/378790
22. Vuppalapati L, Velayudam R, Ahamed KFHN, Cherukuri S, Kesavan R. The protective effect of dietary flavonoid fraction from Acanthophora spicifera on streptozotocin induced oxidative stress in diabetic rats. Food Sci Hum Well 2016;5:57-64.
23. Goth L, Vitai M. Hypocatalasemia in hospital patients. Clin Chem 1996;42:341-2.