EVALUATION OF ANTIDIABETIC, HYPOLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF POLYHERBAL FORMULATION IN STREPTOZOTOCIN-NICOTINAMIDE INDUCED DIABETES IN RATS


Yuvraj Singh Surana, Purnima Ashok, Rajendran R.

Abstract


Objective: To evaluate antidiabetic, hypolipidemic and antioxidant activity of polyherbalformulation (PHF) aqueous extract in streptozotocin-nicotinamide induced diabetes in rats.

Methods: Fasting blood glucose, lipid profiles, serum insulin and glycosylate haemoglobin (HbA1C) were determined in normal and streptozotocin-nicotinamide induced diabetic rats after oral administration of the PHF for 45 d. Antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) levels were evaluated in kidney and liver tissue. Histopathological changes in diabetic rat vital organs were also observed after PHF treatment.

Results: Daily oral administration of PHF (200 and 400 mg/kg, b.w.) and metformin (5 mg/kg, b.w.) showed beneficial effects on blood glucose level (P<0.001) and hyperlipidaemia due to diabetes. The PHF treatment also enhances serum insulin level and body weight of diabetic rats as compared to diabetic control group. Furthermore, the PHF has favourable effects on histopathological studies, in streptozotocin-nicotinamide induced diabetes. Antioxidant enzymes and GSH levels were found to be significantly increased and levels of MDA were decreased in treated diabetic animals.

Conclusion: PHF possesses antidiabetic, hypolipidemic and antioxidant properties. PHF has also showed favourable effect on histopathological changes in streptozotocin-nicotinamide induced diabetic animals.


Keywords


Streptozotocin, Nicotinamide, Metformin, Polyherbal formulation

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About this article

Title

EVALUATION OF ANTIDIABETIC, HYPOLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF POLYHERBAL FORMULATION IN STREPTOZOTOCIN-NICOTINAMIDE INDUCED DIABETES IN RATS

Keywords

Streptozotocin, Nicotinamide, Metformin, Polyherbal formulation

DOI

10.22159/ijpps.2017v9i10.20723

Date

02-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 105-110

Online ISSN

0975-1491

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Authors & Affiliations

Yuvraj Singh Surana
Department of Pharmacology, KLE University College of Pharmacy, Bengaluru KLE Academy of Higher Education and Research (KLE University), Belagavi, Karnataka, India.
India

Purnima Ashok
Department of Pharmacology, KLE University College of Pharmacy, Bengaluru KLE Academy of Higher Education and Research (KLE University), Belagavi, Karnataka, India.

Rajendran R.
Green Chem Herbal Extracts and Formulation, Domlur, Bengluru.


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