PHYTOCHEMICAL ANALYSIS AND IN VITRO STUDIES ON ANTIBACTERIAL, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES USING CASUARINA EQUISETIFOLIA BARK EXTRACTS


Saranya V. T. K., S. Uma Gowrie

Abstract


Objective: Casuarina equisetifolia is an important multipurpose exotic forest tree species widely cultivated in the coastal regions of Tamil Nadu that serves as a warehouse of essential secondary metabolites. Identification of these bioactive compounds in this forest tree species might lead to the discovery and development of a new drug to treat various diseases.

Methods: The present study was carried out with an objective to analyse the phytochemicals qualitatively and quantitatively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to evaluate the presence of various volatile compounds. An in vitro antibacterial, antioxidant and anti-inflammatory properties of aqueous and organic solvents of C. equisetifolia bark was studied.

Results: The preliminary qualitative screening revealed the presence of alkaloids, glycosides, carbohydrates, proteins, flavonoids, phenols, terpenoids, and tannins. The quantitative analysis revealed the presence of maximum phenols (71.2±0.51 mg/g), flavonoids (35.12±0.34 mg/g), tannins (77.59±0.21) and terpenoids (6%) in methanolic root extract with respective standards. Several peaks were obtained in the GC-MS analysis which indicates the presence of different secondary metabolites. Antibacterial activity showed a maximum zone of inhibition against Escherichia coli (23±0.24 mm) and Proteus vulgaris (23±0.32 mm). The antioxidant potential of various extracts was compared with the standard ascorbic acid. Anti-inflammatory activity was compared with standard diclofenac sodium and the extract showed activities significantly in a dose-dependent manner.

Conclusion: From this study, it is revealed that C. equisetifolia bark extract possesses efficient antibacterial property, the potential in scavenging free radical, effective antioxidant, powerful anti-inflammatory source that can be employed in the development of a novel drug to treat various diseases.


Keywords


Casuariana equisetifolia, bark, Phytochemicals, Antioxidant, Antibacterial, Anti-inflammatory, GC-MS

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About this article

Title

PHYTOCHEMICAL ANALYSIS AND IN VITRO STUDIES ON ANTIBACTERIAL, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES USING CASUARINA EQUISETIFOLIA BARK EXTRACTS

Keywords

Casuariana equisetifolia, bark, Phytochemicals, Antioxidant, Antibacterial, Anti-inflammatory, GC-MS

DOI

10.22159/ijpps.2018v10i1.22188

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 118-125

Online ISSN

0975-1491

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61 Views | 97 Downloads

Authors & Affiliations

Saranya V. T. K.
Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008
India

S. Uma Gowrie
Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008
India


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