PHYTOCHEMICAL AND ANTIOXIDANT CHARACTERIZATION OF THINNED IMMATURE CITRUS UNSHIU FRUITS


Ji Hye Kim, Min Young Kim

Abstract


Objective: We aimed to evaluate the characterization of thinned immature Citrus unshiu fruits with regard to their phytochemical profile and antioxidant capacity.

Methods: Determination of total phenolic, flavonoid, and carotenoid and ascorbic acid contents was done by UV-Visible spectrophotometry, whereas UPLC-mass detection was used for the analysis of individual flavanone (naringin, hesperidin, hesperetin, neohesperedine and narirutin) and flavonol (rutin). In addition, free radicals (DPPH, O2-, H2O2 and NO) scavenging assays were used to determine the antioxidant capacity.

Results: Naringin, hesperidine, neohesperedine and narirutin were the main flavanones in all thinned immature Citrus unshiu fruits. The contents of total phenolic, flavonoid and carotenoid were more prevalent in immature fruits than the level found in mature fruits. All thinned immature Citrus unshiu fruits possess an evident antioxidant capacity. The immature Citrus extract concentrations providing 50% inhibition (IC50) for free radicals; 1.2-1.49 mg/ml for DPPH, 1.03-1.46 mg/ml for superoxide, 1.95-3.43 mg/ml for hydrogen peroxide and 1.64-3.45 mg/ml for nitric oxide was lower than those of mature Citrus extracts.

Conclusion: Thinned immature Citrus unshiu fruits could be an economic and readily accessible source of natural antioxidants and as a possible food and pharmaceutical supplement.


Keywords


Thinned immature Citrus, Phytochemical composition, Antioxidant activity

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About this article

Title

PHYTOCHEMICAL AND ANTIOXIDANT CHARACTERIZATION OF THINNED IMMATURE CITRUS UNSHIU FRUITS

Keywords

Thinned immature Citrus, Phytochemical composition, Antioxidant activity

DOI

10.22159/ijpps.2017v9i12.22971

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 12, 2017 Page: 293-297

Online ISSN

0975-1491

Statistics

22 Views | 5 Downloads

Authors & Affiliations

Ji Hye Kim
Toxicology Laboratory, Faculty of Biotechnology (Biomaterials), College of Applied Life Science, SARI, Jeju National University, Jeju, Republic of Korea

Min Young Kim
Toxicology Laboratory, Faculty of Biotechnology (Biomaterials), College of Applied Life Science, SARI, Jeju National University, Jeju, Republic of Korea


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