EVALUATION OF THE EFFICACY OF A HYDROGEN PEROXIDE DISINFECTANT

  • Luz Karime Medina-cÓrdoba Industrial Microbiologist, M.Sc. Georgia Institute of Technology. Atlanta, Georgia
  • Ligia Lucia Valencia-mosquera Industrial Microbiologist Head of Quality Control in Documentary Management EOS. Bogotá, Colombia
  • Gretty Paola Tarazona-diaz Bacteriólogist, Administrative and Marketing Manager at REQPERA I.P.S., S.A.S.
  • Janeth Del Carmen Arias-palacios Bacteriólogist, M.Sc. Environmental and Industrial Biotechnology Group. Department of Microbiology Pontificia Universidad Javeriana. Bogotá, Colombia

Abstract

Objective: To evaluate the efficacy of a disinfectant based on hydrogen peroxide.

Methods: The method used to assess the efficacy of the disinfectant was the agar plate technique. With this procedure, it was possible to determine the percentage of inhibition of the high-level disinfectant of STERIS against four microorganisms, i.e., Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus (Beta-Hemolytic 227), Salmonella choleraesuis (Kuznedorf CMDM 074), and Bacillus subtilis (ATCC 6633). The effectiveness of five disinfectant concentrations (0.02%, 0.04%, 0.08%, 1%, and 2%) was determined and evaluated in three different times 5, 10, and 15 min, for vegetative strains and 3, 6, and 9 h for the sporulated strain.

Results: According to the experimental test, the reduction of the microbial population was, on average, 100% for the disinfectant concentrations of 0.08%, 1%, and 2%.

Conclusion: The results obtained demonstrated that the high-level disinfectant of STERIS based on hydrogen peroxide is 100% effective when the concentration recommended by the commercial house (2%) is used in the shortest time exposure to disinfectant. The minimum level of effectiveness was 0.08%; however, if lower concentrations are used, destruction of the microorganisms is not guaranteed.

Keywords: Bactericidal activity, Effectiveness, Antiseptic, The percentage inhibition

Downloads

Download data is not yet available.

References

1. Cadnum JL, Mana TSC, Jencson A, Thota P, Kundrapu S, Donskey CJ. The effectiveness of a hydrogen peroxide spray for decontamination of soft surfaces in hospitals. Am J Infect Control 2015;12:1357–9.
2. Kanchan C, Sangeeta D, Balasaheb G. A comparative survey of knowledge of antiseptic and disinfectant use and effect of the intervention on medical and surgical staff nurses in a tertiary care hospital. Asian J Pharm Clin Res 2018;11:120-3.
3. Rios Castillo AG, Gonzalez Rivas F, Rodriguez Jerez JJ. Bactericidal efficacy of hydrogen peroxide-based disinfectants against gram-positive and gram-negative bacteria on stainless steel surfaces. J Food Sci 2017;82:2351–6.
4. Assoc Off. Anal Chem AOAC Official Methods of Analysis; 2000.
5. Feridoz J, Roy A. Antibacterial activity of an aqueous alcoholic extract of Abutilon indicum aerial parts against E. faecalis–an in vitro study. Asian J Pharm Clin Res 2017;10:80-1.
6. Uchikawa M, Uchikawa K, Morais F, Quartim C, Queiroz R, Angelo C. Eficacia de la desinfección con alcohol al 70% (p/v) de superficies contaminates sin limpieza previa. Rev Latino Am Enferm 2013;21:1–6.
7. Kohli R, Mittal KL. Developments in surface contamination and cleaning; 2013.
8. Perumal PK, Wand ME, Sutton JM, Bock LJ. Evaluation of the effectiveness of hydrogen-peroxide-based disinfectants on biofilms formed by gram-negative pathogens. J Hosp Infect 2014;87:227–33.
9. Linley E, Denyer SP, McDonnell G, Simons C, Maillard JY. Use of hydrogen peroxide as a biocide: new consideration of its mechanisms of biocidal action. J Antimicrobial Chemother 2012;67:1589–96.
10. Kanemitsu K. A comparative study of ethylene oxide gas, hydrogen peroxide gas plasma, and low-temperature steam formaldehyde sterilization. Infect Control Hosp Epidemiol 2005;26:486–9.
11. Lemmen S, Scheithauer S, Häfner H, Yezli S, Mohr M, Otter JA. Evaluation of hydrogen peroxide vapor for the inactivation of nosocomial pathogens on porous and nonporous surfaces. Am J Infect Control 2015;43:82–5.
12. Horn K, Otter JA. Hydrogen peroxide vapor room disinfection and hand hygiene improvements reduce clostridium difficile infection, methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and extended-spectrum β-lactamase. Am J Infect Control 2015;43:1354–6.
13. Setlow B, Yu J, Li YQ, Setlow P. Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite. Lett Appl Microbiol 2013;57:259–65.
14. Gellatly SL, Hancock REW. Pseudomonas aeruginosa: New insights into pathogenesis and host defenses. Pathog Dis 2013;67:159–73.
15. Arnaouteli S, MacPhee CE, Stanley-wall NR. Just in case it rains: building a hydrophobic biofilm the Bacillus subtilis way. Curr Opinion Microbiol 2016;34:7–12.
Statistics
130 Views | 307 Downloads
Citatons
How to Cite
Medina-cÓrdoba, L. K., L. L. Valencia-mosquera, G. P. Tarazona-diaz, and J. D. C. Arias-palacios. “EVALUATION OF THE EFFICACY OF A HYDROGEN PEROXIDE DISINFECTANT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 10, Oct. 2018, pp. 104-8, doi:10.22159/ijpps.2018v10i10.24652.
Section
Original Article(s)