• Luz Karime Medina-cordoba Industrial Microbiologist, M.Sc. Georgia Institute of Technology. Atlanta, Georgia
  • Ligia Lucia Valencia-mosquera Industrial Microbiologist Head of Quality Control in Documentary Management EOS. Bogotá, Colombia
  • Gretty Paola Tarazona-diaz Bacteriólogist, Administrative and Marketing Manager at REQPERA I.P.S., S.A.S.
  • Janeth Del Carmen Arias-palacios Bacteriólogist, M.Sc. Environmental and Industrial Biotechnology Group. Department of Microbiology Pontificia Universidad Javeriana. Bogotá, Colombia


Objective: To evaluate the efficacy of a disinfectant based on hydrogen peroxide.

Methods: The method used to assess the efficacy of the disinfectant was the agar plate technique. With this procedure, it was possible to determine the percentage of inhibition of the high-level disinfectant of STERIS against four microorganisms, i.e., Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus (Beta-Hemolytic 227), Salmonella choleraesuis (Kuznedorf CMDM 074), and Bacillus subtilis (ATCC 6633). The effectiveness of five disinfectant concentrations (0.02%, 0.04%, 0.08%, 1%, and 2%) was determined and evaluated in three different times 5, 10, and 15 min, for vegetative strains and 3, 6, and 9 h for the sporulated strain.

Results: According to the experimental test, the reduction of the microbial population was, on average, 100% for the disinfectant concentrations of 0.08%, 1%, and 2%.

Conclusion: The results obtained demonstrated that the high-level disinfectant of STERIS based on hydrogen peroxide is 100% effective when the concentration recommended by the commercial house (2%) is used in the shortest time exposure to disinfectant. The minimum level of effectiveness was 0.08%; however, if lower concentrations are used, destruction of the microorganisms is not guaranteed.

Keywords: Bactericidal activity, Effectiveness, Antiseptic, The percentage inhibition


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How to Cite
Medina-cordoba, L. K., L. L. Valencia-mosquera, G. P. Tarazona-diaz, and J. D. C. Arias-palacios. “EVALUATION OF THE EFFICACY OF A HYDROGEN PEROXIDE DISINFECTANT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 10, Oct. 2018, pp. 104-8, doi:10.22159/ijpps.2018v10i10.24652.
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