EVALUATION OF BACTERICIDAL ACTIVITY OF AN ANTISEPTIC GELTHROUGH THE DILUTION-NEUTRALIZATION METHOD

  • Janeth Del Carmen Arias-Palacios Bacteriologist. M. Sc. Grupo Biotecnologia Ambiental e Industrial Departmento de Microbiología. Facultad de Ciencias. Pontificia Universidad Javeriana, Bogota, Colombia
  • LIBARDO HERNANDEZ ESQUIVEL Pharmaceutical Chemist Scientific Manager–Vicar® Bogota, Colombia
  • Dorelly Sandoval Medina Industrial Microbiologist-Pontificia Universidad Javeriana Bogota, Colombia
  • Tatiana Diaz B. Industrial Microbiologist-Pontificia Universidad Javeriana Bogota, Colombia

Abstract

Objective: To evaluate the bactericidal activity of an antiseptic gel for hygiene and disinfection of the hands by rubbing.


Methods: The dilution-neutralization method was used according to Colombian Technical Standard NTC, 2009, 12, 16 in Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC), 5 strains, over 6 different types and with 6 replicas per time were exposed to the antiseptic gel, using Letheen Broth as a neutralizer substance.


Results: A 99% of reduction was obtained with Staphylococcus aureus 6538, Enterococos hirae 10541, Pseudomonas aeruginosa 15442 and Klebsiella sp, within the first 30 s of exposure to the gel and within the first 60 s of exposure using the Escherichia coli 19538. All strains used coming from American Type Culture Collection (ATCC).


Conclusion: It was confirmed that the product is effective. In presence of a neutralizer substance, the microorganisms were not inhibit, so the growing reduction occurs due to the action of the product.

Keywords: Bactericidal activity, antiseptic gel, dilution-neutralization, clean hands

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References

1. WHO. World Health Organization. Guidelines on hand hygiene in health care: first global patient safety challenge clean care is safer care. World Health; 2009.
2. Ochwoto M, Muita L, Talaam K, Wanjala C, Ogeto F, Wachira F, et al. Anti-bacterial efficacy of alcoholic hand rubs in the Kenyan market, 2015. Antimicrob Resist Infect Control 2017;6:1-17.
3. Best EL, Parnell P, Wilcox MH. The potential of alcohol release doorplates to reduce surface contamination during hand contact. J Hosp Infect 2017;97:427-9.
4. ICONTEC NTC Disinfectants and antiseptic chemicals. Quantitative assay of suspension for the evaluation of the bactericidal activity of products for the treatment and hygienic and surgical washing of the hands. Testing Method Icontec International; 2009.
5. Enwa F, Anie C, Oghenejobo M, Ilaya A. Evaluation of the comparative activity of alcohol-based hand sanitizers and toilet soaps against some bacterial isolates. GJSFR: C Biol Sci 2015;15:3.
6. Wirtanen G. Microbiological methods for testing disinfectant efficiency on Pseudomonas biofilm. Colloids Surf B 2001;20:37–50.
7. Alvarez A, Espigares E, Galvez R. Evaluation of disinfectants. Dilution-neutralization method. Hig San Amb 2001;1:1-5.
8. Espigares E, Bueno A, Fernandez M, Espigares M. Efficacy of some neutralizers in suspension tests determining the activity of disinfectants. J Hosp Infect 2003;55:137-40.
9. Bermejo J, Wertz A, Bencomo B, Lesnaberes P, Notario R. Effect of alcohol-gel hand hygiene on nosocomial infections due to multi-resistant Klebsiella pneumoniae. Medicina (B. Aires) 2003;63:715–20.
10. Kampf G, Hollingsworth A. Comprehensive bactericidal activity of an ethanol-based hand gel in 15 seconds. Ann Clin Microbiol Antimicrob 2008;7:2.
11. Asaithambi A, Priyanka N, Snehalaxmi R, Uma A. Health-care associated infections and infection control practices in intensive care unit of a tertiary care hospital. Asian J Pharm Clin Res 2016;9:399-402.
12. David O, Olatunji F, Alese M, Babalola T, Alese O. Antimicrobial activity, safety and acceptability of formulated ginger-fortified hand sanitizer gel. Int J Trop Dis Heal 2017;2:1-11.
13. Alvarado D, Garcia J, Arias M. Evaluation of the effectiveness of alcohol-gel in the disinfection of hands and weakness over time. Biomedica 2010;1:29–31.
14. Mita S, Kusuma S, Insiyah N. Potent antibacterial of gel kawang fruits (Litocarpuscelebicus, (miq) rehder) ethanol extract origin from papua indonesia. Asian J Pharm Clin Res 2017;10:122-7.
15. Lavilla L, Benomar N, Galvez A, Abriouel H. Prevalence of bacteria resistant to antibiotics and/or biocides on meat processing plant surfaces throughout meat chain production. Int J Food Microbiol 2013;161:97–106.
16. Khatoon A, Arzoo A, Mohapatra A, Bihari K. Studies on in vitro evaluation of antibacterial activities of Eucalyptus Globuluslabill leaf. Int J Curr Pharm Res 2017;9:140-2.
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Arias-Palacios, J. D. C., L. H. ESQUIVEL, D. S. Medina, and T. D. B. “EVALUATION OF BACTERICIDAL ACTIVITY OF AN ANTISEPTIC GELTHROUGH THE DILUTION-NEUTRALIZATION METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 2, Dec. 2018, pp. 17-20, doi:10.22159/ijpps.2019v11i2.25716.
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