• Janeth Del Carmen Arias-Palacios Bacteriologist. M. Sc. Grupo Biotecnologia Ambiental e Industrial Departmento de Microbiología. Facultad de Ciencias. Pontificia Universidad Javeriana, Bogota, Colombia
  • LIBARDO HERNANDEZ ESQUIVEL Pharmaceutical Chemist Scientific Manager–Vicar® Bogota, Colombia
  • Dorelly Sandoval Medina Industrial Microbiologist-Pontificia Universidad Javeriana Bogota, Colombia
  • Tatiana Diaz B. Industrial Microbiologist-Pontificia Universidad Javeriana Bogota, Colombia


Objective: To evaluate the bactericidal activity of an antiseptic gel for hygiene and disinfection of the hands by rubbing.

Methods: The dilution-neutralization method was used according to Colombian Technical Standard NTC, 2009, 12, 16 in Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC), 5 strains, over 6 different types and with 6 replicas per time were exposed to the antiseptic gel, using Letheen Broth as a neutralizer substance.

Results: A 99% of reduction was obtained with Staphylococcus aureus 6538, Enterococos hirae 10541, Pseudomonas aeruginosa 15442 and Klebsiella sp, within the first 30 s of exposure to the gel and within the first 60 s of exposure using the Escherichia coli 19538. All strains used coming from American Type Culture Collection (ATCC).

Conclusion: It was confirmed that the product is effective. In presence of a neutralizer substance, the microorganisms were not inhibit, so the growing reduction occurs due to the action of the product.

Keywords: Bactericidal activity, antiseptic gel, dilution-neutralization, clean hands


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How to Cite
Arias-Palacios, J. D. C., L. H. ESQUIVEL, D. S. Medina, and T. D. B. “EVALUATION OF BACTERICIDAL ACTIVITY OF AN ANTISEPTIC GELTHROUGH THE DILUTION-NEUTRALIZATION METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 2, Feb. 2019, pp. 17-20, doi:10.22159/ijpps.2019v11i2.25716.
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