LYCOPENE PRODUCTION IN MYCOBACTERIUM SMEGMATIS BY EXPRESSION OF CRT GENES FROM MYCOBACTERIUM AURUM AND PROTECTIVE EFFECT OF LYCOPENE IN VIVO AND IN VITRO AGAINST UV RADIATION

  • Oumaima Elamin Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
  • Marwa Chraibi Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
  • Saad Koraichi Ibnsouda Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
  • Mohammed Houssaini Iraqui Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco

Abstract

Objective: The aim of the present study was to express in Mycobacterium smegmatis the clustered mycobacterial genes coding for lycopene synthesis and to investigate the protective properties of lycopene against ultraviolet (UV) irradiation.

Methods: The genes, which encode the biogenesis of lycopene in Mycobacterium aurum A+, were introduced into Mycobacterium smegmatis by electroporation. The pigments produced were analyzed by thin layer chromatography, and the absorption spectra were determined. A survival test using UV irradiations was also performed.

Results: The transformed Mycobacterium smegmatis were found to synthesize lycopene with important yield (1.41± 3.09 mg/g) and was more resistant to ultraviolet irradiation than non-pigmented strain (p<0.01). Furthermore, cells of M. smegmatis not transformed but coated with lycopene are more resistant to UV than those uncoated (p<0.01).

Conclusion: M. smegmatis can form orange colonies on agar plates when it is transformed with the lycopene genes, and the transformants produces 1.41 mg/g (dry weight) of this carotene. Our findings strongly suggest that lycopene has antioxidant activities and prevent the lethal action of UV irradiation on bacterial cells in vivo and in vitro, and deserves further studies considering the amelioration of the production.

Keywords: Mycobacterium smegmatis, Lycopene, Plasmid, UV irradiation

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Author Biographies

Oumaima Elamin, Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
Laboratory of Microbial Biotechnology. Department of Life Sciences, Faculty of Sciences and Technology (FST) Fez, Sidi Mohamed Ben abdellah University. BP 2202 Fes Maroc
Marwa Chraibi, Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
Laboratory of Microbial Biotechnology. Department of Life Sciences, Faculty of Sciences and Technology (FST) Fez, Sidi Mohamed Ben abdellah University. BP 2202 Fes Maroc
Saad Koraichi Ibnsouda, Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
Laboratory of Microbial Biotechnology. Department of Life Sciences, Faculty of Sciences and Technology (FST) Fez, Sidi Mohamed Ben abdellah University. BP 2202 Fes Maroc
Mohammed Houssaini Iraqui, Sidi Mohamed Ben Abdelah University, Faculty of Sciences and Technologies, Department of Microbiology, B.P. 2202, Imouzzer Street, Fes, Morocco
Professor Iraqui, Laboratory of Microbial Biotechnology. Department of Life Sciences, Faculty of Sciences and Technology (FST) Fez, Sidi Mohamed Ben abdellah University. BP 2202 Fes Maroc

References

1. Armstrong GA. Genetics of eubacterial carotenoid biosynthesis: a colorful tale. Annu Rev Microbiol 1997;51:629-59.
2. Sugawara T, Yamashita K, Asai A, Nagao A, Shiraishi T, Imai I, et al. Esterification of xanthophylls by human intestinal caco-2 cells. Arch. Biochem Biophys 2009;483:205-12.
3. Amengual J, Lobo GP, Golczak M, Li HNM, Klimova T, Hoppel CL, et al. A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 2011;25:948-59.
4. Hernandez-Almanza A, Montanez J, Martinez G, Aguilar-Jimenez A, Contreras-Esquivel JC, Aguilar CN. Progress in microbial production. Trends Food Sci Technol 2016;56:142-8.
5. Schnurr G, Schmidt A, Sandmann G. Mapping of a carotenogenic gene cluster from Erwinia herbicola and functional identification of six genes. FEMS Microbiol Lett 1991;78:157-61.
6. Chamovitz D, Misawa N, Sandmann G, Hirschberg J. Molecular cloning and expression in Escherichia coli of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzyme. FEBS Lett 1992;296:305-10.
7. Krügel H, Krubasik P, Weber K, Saluz HP, Sandmann G. Functional analysis of genes from Streptomyces griseus involved in the synthesis of isorenieratene, a carotenoid with aromatic end groups, revealed a novel type of carotenoid desaturase. Biochim Biophysica Acta 1999;1439:57-64.
8. Araya Garay JM, Feijoo Siota L, Veiga Crespo P, Villa TG. cDNA cloning of a novel gene codifying for the enzyme lycopene β-cyclase from Ficus carica and its expression in Escherichia coli. Appl Microbiol Biotechnol 2011;92:769-77.
9. Suwanaruang T. Analyzing lycopene content in fruits. Agric Agric Sci Proc 2016;11:46-8.
10. Di Mascio P, Kaiser S, Sies H. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 1989;274:532-8.
11. Sahin K, Yazlak H, Orhan C, Tuzcu M, Akdemir F, Sahin N. The effect of lycopene on antioxidant status in rainbow trout (Oncorhynchus mykiss) reared under high stocking density. Aquaculture 2014;418:132-8.
12. Soares NDCP, Teodoro AJ, Oliveira FL, Santos CADN, Takiya CM, Junior OS, et al. Influence of lycopene on cell viability, cell cycle, and apoptosis of human prostate cancer and benign hyperplastic cells. Nutr Cancer 2013;65:1076-85.
13. Campos KKD, Araujo GR, Martins TL, Bandeira ACB, de Paula Costa G, Talvani A, et al. The antioxidant and anti-inflammatory properties of lycopene in mice lungs exposed to cigarette smoke. J Nutr Biochem 2017;48:9-20.
14. Hazewindus M, Haenen GRMM, Weseler AR, Bast A. The anti-inflammatory effect of lycopene complements the antioxidant action of ascorbic acid and α-tocopherol. Food Chem 2012;132: 954-8.
15. Sung WS, Lee IS, Lee DG. Damage to the cytoplasmic membrane and cell death caused by lycopene in Candida albicans. J Microbiol Biotechnol 2007;17:1797-804.
16. Das A, Yoon SH, Lee SH, Kim JY, Oh DK, Kim SW. An update on microbial carotenoid production: application of recent metabolic engineering tools. Appl Microbiol Biotechnol 2007; 77:505-12.
17. Nobre BP, Gouveia L, Matos PG, Cristino AF, Palavra AF, Mendes RL. Supercritical extraction of lycopene from tomato industrial wastes with ethane. Molecules 2012;17:8397-407.
18. Lopez Nieto MJ, Costa J, Peiro E, Mendez E, Rodriguez Saiz M, De la Fuente JL, et al. Biotechnological lycopene production by mated fermentation of Blakeslea trispora. Appl Microbiol Biotechnol 2004;66:153-9.
19. Snapper SB, Melton RE, Mustafa S, Kieser T, Jacobs WR. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol Microbiol 1990;4:1911-9.
20. Hassi M, Haggoud A, El Mzibri M, Ibnsouda S, Houari A, Iraqui M. Isolation and identification of a staphylococal strain with an anti-mycobacterial activity and study of its mode of action. Ann Microbiol 2007;57:651-6.
21. Papa F, Riviere M, Fournie JJ, Puzo G, David H. Specificity of a Mycobacterium kansasii phenolic glycolipid (mycoside A) immune serum. J Clin Microbiol 1987;25:2270-3.
22. Houssaini Iraqui M, Khamlichi N, El Yamani J, Rastogi N. Response of Escherichia coli Containing mycobacterial carotene genes to UV radiation. J Biomed Biotechnol 2001;1:79-84.
23. Houssaini Iraqui M, Lazraq MH, Clavel Seres S, Rastogi N, David HL. Cloning and expression of Mycobacterium aurum carotenogenesis genes in Mycobacterium smegmatis. FEMS Microbiol Lett 1992;69:239-44.
24. Lu C, Engelmann NJ, Lila MA, Jr JWE. Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology. J Agric Food Chem 2008;56:7710-4.
25. Dagnelie P. Statistiques theoriques et appliquees–tome 1 statistique descriptive et bases de l’inférence statistique; 1998.
26. Ausich RL. Commercial opportunities for carotenoid production by biotechnology. Pure Appl Chem 1997;69:2169-73.
27. Zhu F, Lu L, Fu S, Zhong X, Hu M, Deng Z, et al. Targeted engineering and scale-up of lycopene overproduction in Escherichia coli. Process Biochem 2015;50:341-6.
28. Shore RE. Radiation-induced skin cancer in humans. Med Pediatr Oncol 2001;36:549-54.
29. Won EJ, Han J, Lee Y, Kumar KS, Shin KH, Lee SJ, et al. In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana. Aquat Toxicol 2015;165:1-8.
30. Butnariu MV, Giuchici CV. The use of some nanoemulsions based on aqueous propolis and lycopene extract in the skin’s protective mechanisms against UVA radiation. J Nanobiotechnol 2011;9:3.
31. Bhat AH, Dar KB, Anees S, Zargar MA, Masood A, Sofi MA, et al. Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases; a mechanistic insight. Biomed Pharmacother 2015;74:101-10.
32. Mei Y, Thompson MD, Cohen RA, Tong X. Autophagy and oxidative stress in cardiovascular diseases. Biochim Biophys Acta 2015;1852:243-51.
33. Martinez A, Melendez Martinez AJ. Lycopene, oxidative cleavage derivatives, and antiradical activity. Comput Theor Chem 2016;1077:92-8.
34. Srinivasan M, Kalpana KB, Devipriya N, Menon VP. Protective effect of lycopene on whole body irradiation induced liver damage of swiss albino mice: a pathological evaluation. Biomed Prev Nutr 2014;4:87-94.
35. Lee LK, Foo KY. An appraisal of the therapeutic value of lycopene for the chemoprevention of prostate cancer: a nutrigenomic approach. Food Res Int 2013;54:1217-28.
36. Arab L, Steck S. Lycopene, and cardiovascular disease. Am J Clin Nutr 2000;71:1691S-5S.
37. Prema A, Janakiraman U, Manivasagam T, Thenmozhi AJ. Neuroprotective effect of lycopene against MPTP induced experimental Parkinson’s disease in mice. Neurosci Lett 2015;599:12-9.
38. Anitha T. Medicinal plants used in skin protection. Asian J Pharm Clin Res 2012;5 Suppl 3:35-8.
39. Priyanka S, Mary SRI, Nandini HS, Kutty AVM, Kiranmayee P. A pilot study on sun protection factor of plant extracts: an observational study. Asian J Pharm Clin Res 2018;11:67-71.
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How to Cite
Elamin, O., M. Chraibi, S. K. Ibnsouda, and M. H. Iraqui. “LYCOPENE PRODUCTION IN MYCOBACTERIUM SMEGMATIS BY EXPRESSION OF CRT GENES FROM MYCOBACTERIUM AURUM AND PROTECTIVE EFFECT OF LYCOPENE IN VIVO AND IN VITRO AGAINST UV RADIATION”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 9, Sept. 2018, pp. 49-53, doi:10.22159/ijpps.2018v10i9.25768.
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