• Nezha Mattazi Sidi Mohamed Ben Abdellah University
  • Abdellah Farah Sidi Mohamed Ben Abdellah University
  • Mouhcine Fadil Sidi Mohamed Ben Abdellah University
  • Marwa Chraibi Sidi Mohamed Ben Abdellah University
  • Kawtar Fikri Benbrahim Laboratory of Microbial Biotechnology, Faculty of Science and Technology Saïss. Sidi Mohamed Ben Abdellah University, Fez, Morocco


Objective: This study was designed to determine the chemical composition and the antibacterial activity of hydro-distillated essential oils (EOs) extracted from leaves of three aromatic and medicinal plants cultivated in Agadir (Morocco): Rosmarinus officinalis, Mentha piperita and Salvia officinalis.

Methods: EOs chemical composition was determined by GC and GC/MS. Disc diffusion method and agar dilution technique were used to evaluate their antibacterial activity against eight microbial strains. Moreover, a statistical study, consisting in a student test, was conducted.

Results: The most important constituents identified were α-pinene (34.83%), a-thujone (24.05%) and Menthol (41, 23%) respectively in the Rosemary, the Sage and Mint. All tested EOs exhibited an antibacterial activity at different levels against the studied strains. Micrococcus luteus was the most sensitive to the tested EOs with Minimum Inhibitory Concentration values of 5.8, 2.35 and 20 mg/ml for Rosemary, Sage and Mint EOs respectively. Thus, these EOs can be used as antibacterial supplement in the developing countries to develop new therapeutic agents.

Conclusions: Additional in vivo studies and clinical trials would be needed to justify and further evaluate their potential.


Keywords: Antibacterial activity, Essential oils, Rosmarinus officinalis, Salvia officinalis, Mentha piperita


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How to Cite
Mattazi, N., A. Farah, M. Fadil, M. Chraibi, and K. F. Benbrahim. “ESSENTIAL OILS ANALYSIS AND ANTIBACTERIAL ACTIVITY OF THE LEAVES OF ROSMARINUS OFFICINALIS, SALVIA OFFICINALIS AND MENTHA PIPERITA CULTIVATED IN AGADIR (MOROCCO)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 73-79,
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