EFFECT OF SOLVENT EXTRACTION SYSTEM ON THE ANTIOXIDANT ACTIVITIES OF ALGAE
Objective: The present study aimed to determine the antioxidant activities of five algae including Oedogonium globossum, Pithophora oedogonia, Rhizoclonium hieroglyphicum, Scytonema ocellatum and Spirogyra communis and the effect of different solvent (benzene, chloroform, acetone and methanol) used for the extraction of active components from algae.
Methods: The five algae were extracted with four different solvents e. g benzene, chloroform, acetone and methanol. The Folin-Ciocalteu procedure was used to estimate the amount of total phenolic content in different extracts of algae. Total flavonoid content was estimated by aluminium chloride colorimetric method. The DPPH radical scavenging assay was used to find out the antioxidant activities of algae under investigation.
Results: The total phenolic content varied from 9.80Â±0.29 to 44.44Â±2.26 mg/g, 7.26Â±0.08 to 21.30Â±0.17 mg/g, 9.82Â±0.12 to 50.14Â±0.99 mg/g and 11.51Â±0.17 to 54.02Â±0.58 mg/g dry material in the benzene, chloroform, acetone and methanol extracts of the algae respectively. The flavonoid content was found to be the highest in the acetone extract of Scytonema ocellatum (593.81Â±2.69 mg/g) and flavonol content was found to be the highest in the chloroform extract of Scytonema ocellatum (448.22 Â± 4.36 mg/g) whereas least amount of flavonoid present in the benzene extract of Oedogonium globossum 8.91Â±0.37 and least amount of flavonol was present in the chloroform extract of Spirogyra communis (13.54Â±1.07 mg/g). The highest DPPH radical scavenging was observed in the methanol extract of Scytonema ocellatum with IC50 = 0.24Â±0.003 mg dry material. The reducing power of the extracts of the algae was also evaluated as mg AAE (ascorbic acid equivalent)/g dry material. Chloroform extract of Rhizoclonium hieroglyphicum showed the highest reducing power 185.56Â±2.00 mg AAE/g dry materials.
Conclusion: Thus it could be suggested that these algae can be utilized as natural antioxidant.
2. Udhayasankar MR, Danya U, Arumugasamy K. Phytochemistry and free radical scavenging activity of Wattakaka volubilis (Linn. f.) Benth ex. Hook f. (Asclepiadaceae)-A rare and threatened medicinal plant. Int J Pharm Tech Res 2012;4(3):1025-32.
3. Patel VR, Patel PR, Kajal SS. Antioxidant activity of some selected medicinal plants in western region of India. Adv Biol Res 2010;4(1):23-6.
4. Hasan NS, Amom ZH, Nor AI, Mokhtarrudin N, Mohd Esa N, Azlan A. Nutritional composition and in vitro evaluation of the antioxidant properties of various dates extracts (Phoenix dactylifera L.) from Libya. Asian J Clin Nut 2010;2:208-14.
5. Kaur N, Kishore L. Antioxidant activity of methanolic extract of Phaseolus trilobus root powder. Int J Pharm Pharm Sci 2012;4(1):271-5.
6. Krishnaraj RN, Babu SV, Ashokkumar B, Malliga P, Varalakshmi P. Antioxidant property of fresh and marine water cyanobacterial extracts in Swiss mice. J Biopest 2012;5:250-4.
7. Indu H, Seenivasan R. In vitro antioxidant activity of selected seaweeds from southeast coast of india. Int J Pharm Pharm Sci 2013;5(2):474-84.
8. Supardya NA, Ibrahima D, Sulaimanb SF, Zakaria NA. Free radical scavenging activity, total phenolic content and toxicity level of Halimeda discoidea (decaisne) extracts (Malaysiaâ€™s green macroalgae) Int J Pharm Pharm Sci 2011;3(5):397-402.
9. Singleton VL, Rossi JA. Colorimetry of total phenolics with Phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
10. Ordonez AAL, Gomez JG, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chem 2006;97:452-8.
11. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006;97:109-14.
12. Oyaizu M. Studies on product on browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
13. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958;181:1199-200.
14. Florence OJ, Adeolu AA, Anthony JA. Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchus oleraceus. Rec Nat Prod 2011;5(1):29-42.
15. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.
16. Jamuna KS, Ramesh CK, Srinivasa TR, Raghu KI. In vitro antioxidant studies in some common fruits. Int J Pharm Pharm Sci 2011;3(1):60-3.
17. Koleva II, Van Beek TA, Linssen JPH, Groot AD, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002;13:8-17.