EFFECT OF BOILING AND MICROWAVE COOKING ON NUTRITIONAL, ANTI-NUTRITIONAL AND TOXICITY OF WILD EDIBLE PLANTS OF NORTH-EASTERN REGION IN INDIA
Objective: The target of this appraisal was to explore the impacts of various cooking techniques, for example, boiling and microwave cooking on nutritional, antinutritional, minerals content and in vitro toxicity of ten wild consumable plants viz. Zanthoxylum acanthopodium, Viburnum foetidum, Houttuynia cordata, Sonchus arvensis, Oenanthe linearis, Perilla ocymoides, Clerodendrum colebrookeanum, Solanum gilo, Solanum kurzii, Potentilla lineata, widely consumed by the common individuals of North-Eastern area in India.
Methods: The proximate parameters like ash, moisture, protein, fat, fibre, carbohydrate, energy content, minerals viz. sodium, calcium, potassium, iron, magnesium, manganese, copper, zinc and antinutritional parameters like oxalate, phytate, tannin, saponin, cyanogenic glycoside content were evaluated in the selected wild edible plants using standard food analysis techniques. In vitro haemolytic toxicity of aqueous extracts (100, 300 and 500µg/cc) of ten palatable wild plants was done with the blood samples were gathered from healthy rat, mixed with Ethylenediaminetetraacetic acid(EDTA) and centrifuged at 5,000 Revolutions Per Minute (rpm) for five minutes. The 10 % erythrocyte suspension was set up in sterile Phosphate buffer saline (PBS, pH 7.4) for haemolytic examination. The genotoxic potential of the concentrates were assessed by a single-cell gel electrophoresis comet test. Cytotoxicity studies were evaluated with fresh goat livers procured from the local market were perfused in PBS (pH 7.4) with collagenase and the liver was then minced in minute pieces and cells were isolated utilizing cell strainer.
Results: Both cooking medications diminished the congregations of ash, fat, minerals, antinutritional parts and the destructive nature of the consumable plants while the carbohydrate and fiber substance were expanded. The protein focuses in the wild edibles were expanded fundamentally (P<0.05) in the range from 1.26 to 10.45% on microwave cooking while the indistinguishable were exhausted in the range out of 2.20 to11.55% on boiling treatment. The microwave cooking demonstrated lesser misfortunes in minerals in the consumable plants than those cooked by frothing. The microwave cooking also caused the colossal rot (P<0.05) of antinutritional parameters and damaging tendency to a more significant degree than the sputtering medications of the wild edibles.
Conclusion: Therefore, the outcomes uncovered that microwaving of appealing plants could be prescribed to expand the supporting quality and to diminish the fat, threatening to dietary structure and lethality. The toxicity assessment of the consumable plants at cell and genomic level showed that these are harmless to consume.
2. Aberoumand A. Screening of less known two food plants for comparison of nutrient contents: Iranian and Indian vegetables. Funct Food Health Dis 2011;10:416-23.
3. Basu S, Das M, SEN A, Choudhury UR, Datta G. Analysis of the complete nutritional profile of Amorphophallus campanulatus tuber cultivated in Howrah district of West Bengal, India. Asian J Pharm Clin Res 2014;7:25-9.
4. Liener IE, Kkade ML. Protease inhibitor. In: Liener IE. (ed). Toxic constituents of plants foodstuffs. Academic Press. New York; 1980.
5. Larsgon M, Rossande Hulthen L, Sandstome B, Sandberg A. Improved iron and zinc absorption from breakfast meals containing malted oats with reduced phytate content. Br J Nutr 1996;76:677-88.
6. Reddy NR, Sathe SK, Pierson MD. Removal of phytate from great northern beans (Phaseous vulgaris L.) and its combined density fraction. J Food Sci 1988;53:107–10.
7. Hahn DH, Rooney LW, Earp CF. Tannins and phenols of sorghum. Cereal Food World 1984;29:776–9.
8. Plewa MJ, Wagner ED. Activation of promutagens by green plants. Annu Rev Genet 1993;27:93–113.
9. Kshirsagar PP, Bhogaonkar PY. Nicandra physaloides (L.) gaertn–a less known wild edible fruit. Int J Curr Pharm Res 2015;7:60-3.
10. Hefnawy TH. Effect of processing methods on nutritional composition and anti-nutritional factors in lentils (Lens culinaris). Ann Agric Sci 2011;2:57–61.
11. AOAC. Association of official analytical chemists, seventeenth. ed. Washington DC Arlington, Virginia, USA; 2000.
12. Hedge JE, Hofreiter BT. Determination of reducing sugars and carbohydrates. In: Whistler RL., Be Miller JN. Eds. Methods in Carbohydrate Chemistry. Academic Press, New York; 1962. p. 380-94.
13. Gawalko EJ, Nowicki TW, Babb J, Tkachuk R. Comparison of closed vessel and focused open-vessel microwave dissolution for determination of cadmium, copper, lead, and selenium in wheat, wheat products, corn bran, and rice flour by transverse-heated graphite furnace atomic absorption spectrometry. J AOAC Int 1997;80:379–87.
14. Munro A, Bassir O. Oxalate in Nigerian vegetables. West Afr J Biol Appl Chem 1980;12:14-8.
15. Reddy MB, Love M. The impacts of food processing on the nutritional quality of vitamins and minerals. Adv Exp Med Biol 1999;459:99-106.
16. Hudson BJF, El-Difrawi EA. The sapogenins of the seeds of four lupin species. J Plant Foods 1979;3:181-6.
17. Price ML, Scoyoc SV, Butler LG. A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 1978;26:1214–8.
18. Gajjar DG, Patel RM, Patel VA, Patel PKM. Novel hydroxyl-terminated dendrimers as potential drug carriers: sustained release, hemolysis and cytotoxicity study. Int J Appl Pharm 2015;7:5-9.
19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
20. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184–91.
21. Gopalan C, Rama Sastri BV, Balasubramanian SC. Nutritive value of Indian Foods. Printed by national institute of nutrition, Indian Council of Medical Research, Hyderabad-500 007, India; 2004. p. 2-58.
22. Satter MMA, Khan MMRL, Jabin SA, Abedin N, Islam MF, Shaha B. Nutritional quality and safety aspects of wild vegetables consume in Bangladesh. Asian Pac J Trop Biomed 2016;6:125-31.
23. Seal T, Chaudhuri K, Pillai B. Evaluation of proximate and mineral composition of wild edible leaves, traditionally used by the local people of Meghalaya state in India. Asian J Plant Sci 2013;12:171-5.
24. Seal T, Pillai B, Chaudhuri K. Evaluation of nutritional potential of five unexplored wild edible plants consumed by the tribal people of Arunachal Pradesh State in India. J Food Nutr Res 2017;5:1-5.
25. Narzary H, Swargiary A, Basumatary S. Proximate and vitamin C analysis of wild edible plants consumed by Bodos of Assam, India. J Mol Pathophysiol 2015;4:128-33.
26. Tsungai R, Munyanyi M, Mduluza T. Effect of cooking and preservation on the nutritional and phytochemical composition of the mushroom Amanita zambiana. Food Sci Nutr 2017;5:538-44.
27. Jenkin DJ, Jenkin AL, Wolever TMS, Rao AV, Thompson LU. Fibre and starchy foods: gut function and implication in disease. Am J Gastroenterol 1986;81:920-30.
28. Trumbo P, Schlicker S, Yates AA, Poos M. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids. J Am Diet Assoc 2002;102:1621-30.
29. Rehman NU, Hussain J, Ali L, Khan AL, Mabood F, Gillani SA, et al. Nutritional assessment and mineral composition of some selected edible vegetables. Euro J Med Plants 2014;4:444-57.
30. Vadivel V, Janardhanan K. Nutritional and anti-nutritional characteristics of seven South Indian wild legumes. Plant Food Hum Nutr 2005;60:69-75.
31. Gupta S, Lakshmi AJ, Manjunath MN, Prakash J. Analysis of nutrient and antinutrient content of underutilized green leafy vegetables. LWT Food Sci Technol 2005;38:339-45.
32. Ali A. Proximate and mineral composition of the marchubeh (Asparagus officinalis). World J Dairy Food Sci 2009;4:142-9.
33. Saha J, Biswal AK, Deka SC. Chemical composition of some underutilized green leafy vegetables of Sonitpur district of Assam, India. Int Food Res J 2015;22:1466-73.
34. Bressani T. Grain quality of common beans. Food Rev Int 1993;9:237–97.
35. Bliss FA. Cowpeas in Nigeria: Nutritional improvement of food legumes Breeding, Milner M. ed. New York: John Wiley and Sons; 1975.
36. Saupi N, Zakaria MH, Bujang JS. Analytic chemical composition and mineral content of yellow velvet leaf (Limnocharis flava (L.) Buchenau)’s edible parts. J Appl Sci 2009;9:2969-74.
37. Sundriyal M, Sundriyal RC. Wild edible plants of the Sikkim Himalaya: nutritive values of selected species. Econ Bot 2004;58:286-99.
38. Mohammed MI, Sharif N. Mineral composition of some leafy vegetables consumed in Kano, Nigeria. Nig J Basic Appl Sci 2011;19:208-12.
39. Geissler CA, Powers HJ. Human nutrition, eleventh edition. Elsevier, Churchill Livingstone; 2005.
40. Chaturvedi VC, Shrivastava R, Upreti RK. Viral infections and trace elements: a complex trace element. Curr Sci 2004;87:1536-54.
41. Indrayan AK, Sharma S, Durgapal D, Kumar N, Kumar M. Determination of nutritive value and analysis of mineral elements for some medicinally valued plants from Uttaranchal. Curr Sci 2005;89:1252-5.
42. Saikia P, Deka DC. Mineral content of some wild green leafy vegetables of North-East India. J Chem Pharm Res 2013;5:117-21.
43. Ilelaboye NOA, Amoo IA, Pikuda OO. Effect of cooking methods on mineral and anti-nutrient composition of some green leafy vegetables. Arch Appl Sci Res 2013;5:254-60.
44. Yasir A, Ahmad A. Impact of processing on nutritional and antinutritional factors of legumes: a review. Ann Food Sci Technol 2018;19:199-215.
45. Sharma N, Goyal SK, Alam T, Fatma S, Chaoruangrit A, Niranjan K. Effect of high pressure soaking on water absorption, gelatinization, and biochemical properties of germinated and non-germinated foxtail milletgrains. J Cereal Sci 2018;83:162-70.
46. Fan Y, Guo DY, Song Q, Li T. Effect of total saponin of aralia taibaiensis on the proliferation of leukemia cells. Zhong Yao Cai 2013;36:604-7.
47. Fereidoon S, Wanasundara PKJPD. Cyanogenic glycosides of flaxseeds. Antinutrients and Phytochemicals in Food. Chapter 10; 1997. p. 171-85.
48. Behravan J, Mosafa F, Soudmand N, Taghiabadi E, Razavi BM, Karimi G. Protective effects of aqueous and ethanolic extracts of Portulaca oleracea L. aerial parts on H2O2-induced DNA damage in lymphocytes by comet assay. J Acupunct Meridian Stud 2011;4:193-7.
49. Kumari N, Deshwal RK. Antioxidants and their protective action against DNA Damage. Int J Pharm Pharm Sci 2011;3:28-32.
50. Mohamed AM, Cangiano MA, Alcaraz LE, Satorres SE, Laciar AL, Mattana CM. Comet assay application in evaluation the safe use of medicinal plants. Emirates J Food Agric 2016;28:737-40.
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