VASCULAR ENDOTHELIAL GROWTH FACTOR VEGF G/C 405 AND C/A 2578 GENE POLYMORPHISMS IN CASES WITH PRE-ECLAMPSIA
Vascular endothelial growth factor gene plays a crucial role in physiological vasculogenesis and vascular permeability and has been implicated in the pathogenesis of pre-eclampsia (PE). VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. This study carried on 170 pregnant women. Out of them, 100 cases with PE. Their mean age Â± SD was 25.28 Â± 4.59 years. Seventy nine (79%) cases had mild PE and 21 (21%) cases had severe PE. The other 70 subjects were clinically healthy pregnant women with complete successful pregnancy and no PE. VEGF C 405 G and C 2578 A polymorphisms were studied using PCR and RFLP. We found that PE among Egyptian women was strongly associated with pre-eclamptic mutations related to VEGF C 405 G and C 2578 A gene polymorphisms. There were statistically significant low frequencies of wild homozygous genotype CC and high frequencies of heterozygous mutant genotypes CG & CA and homozygous mutant genotypes GG & AA in PE cases compared to controls. The frequency of cases carrying the homozygous mutant genotype GG was higher among severe pre-eclamptic cases compared to mild pre-eclamptic cases. In addition, there were statistically significant high frequencies in PE cases compared to controls of mutant G and mutant A alleles.
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