CHARACTERIZATION, IN VITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF FUNGAL ENDOPHYTIC EXTRACTS OF ANDROGRAPHIS PANICULATA LEAVES IN CCL4 INDUCED HEPATOTOXICITY

  • Smita K. Puri PG Depatment of Pharmacognosy and Phytochemistry, SET’s College of Pharmacy, S R Nagar, Dharwad 580002, Karnataka, India,
  • Prasanna V. Habbu PG Depatment of Pharmacognosy and Phytochemistry, SET’s College of Pharmacy, S R Nagar, Dharwad 580002, Karnataka, India,
  • Preeti V. Kulkarni PG Department of Pharmacology, SET’s College of Pharmacy, S R Nagar, Dharwad 580002, Karnataka, India
  • Venkatrao H. Kulkarni PG Department of Pharmacology, SET’s College of Pharmacy, S R Nagar, Dharwad 580002, Karnataka, India

Abstract

Objective: To isolate and characterize the endophytic fungi from the leaves of Andrographis paniculata for free radical scavenging antioxidant and hepatoprotective activity against CCl4 induced hepatotoxicity


Methods: Two fungal endophytes, APLF-3 (Andrographis paniculata leaf fungi-3) and APLF-4 (Andrographis paniculata leaf fungi-4) were isolated from leaves of Andrographis paniculata to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA sequencing by PCR technique was carried out for identification of APLF-3 and APLF-4. All the APLF-3 and APLF-4 extracts were assayed for in vitro free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power. Then, A4EA and A4nB were screened for hepatoprotective activity against CCl4 induced hepatotoxicity at 50 mg/kg and100 mg/kg doses.


Results: The endophytic fungi, APLF-3 and APLF-4, were identified as Phyllosticta sp. ZLY-2010 isolate M13 and Aspergillus tubingensis strain Cs/7/2 respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant in vitro free radical scavenging activity as compared to other extracts. A4EA and A4nB (50 mg/kg and100 mg/kg) reversed the increased serum biochemical parameters as compared to CCl4 treated group (p<0.001). A4EA and A4nB (100 mg/kg p. o) also restored the LPO, SOD and CAT levels.


Conclusion: These findings suggested that the extracts (A4EA and A4nB) obtained from endophytic fungi APLF-4 contributed towards hepatoprotective activity.

Keywords: Endophytic fungi, Hepatoprotective, Antioxidant, Andrographis paniculata, Phyllosticta sp. ZLY-2010 isolate M13, Aspergillus tubingensis strain Cs/7/2

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Puri, S. K., P. V. Habbu, P. V. Kulkarni, and V. H. Kulkarni. “CHARACTERIZATION, IN VITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF FUNGAL ENDOPHYTIC EXTRACTS OF ANDROGRAPHIS PANICULATA LEAVES IN CCL4 INDUCED HEPATOTOXICITY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 1, Jan. 2019, pp. 44-54, doi:10.22159/ijpps.2019v11i1.26832.
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