PHARMACODYNAMIC INTERACTION OF TINOSPORA CORDIFOLIA WITH ATENOLOL AND PROPRANOLOL IN ISOPROTERENOL INDUCED CARDIAC NECROSIS IN RATS

  • Chetan savant SETs College of Pharamcy, Dharwad
  • VENKATRAO KULKARNI Department of Pharmacology, SET’s College of Pharmacy, Dharwad, Karnataka-580002, India
  • PRASANNA HABBU Department of Pharmacognosy, SET’s College of Pharmacy, Dharwad, Karnataka-580002, India

Abstract

ABSTRACT


Objective: In the present scenario high rate of mortality is mainly because of cardiovascular diseases globally.  Hence, there is need for extensive research for effective treatment of these cardiovascular diseases. The present study was carried out with aim to evaluate the combined cardioprotective effect of standardized extract of Tinospora cordifolia extract (TCE) with atenolol (AT) and propranolol (PP) in isoproterenol (ISO) induced cardiac necrosis in rats. Methods: Myocardial infarction (MI) or cardiac necrosis was induced by subcutaneous administration of ISO for two days consecutively at an interval of 24 h. Rats were pre-administered with test drugs for  21 days followed by ISO was administration on 20 and 21st day. 24 h after final ISO administration mean arterial blood pressure (MAB), Heart rate (HR), electrocardiogram (ECG), heart bio-marker enzyme and histopathological study of cardiac tissue were evaluated from control and experimental groups and analyzed statistically by one-way ANOVA followed by Tukeys’s test.


Results: Rats administered with ISO showed significant changes in ECG, HR, MAB, heart bio-marker enzyme, antioxidant parameters and histopathology of heart. The activities of biomarkers have reduced in serum and there is increase antioxidants in heart tissue of drug treated animals. Similarly, ECG, MAB and HR were restored to normalcy in drug treated animals.


Conclusion: It may be concluded that the herb-drug combinations i.e TCE (500 mg/kg) + AT (10 mg/kg) and TCE (500 mg/kg) + PP (10 mg/kg) have shown increased cardioprotective activity than they were used alone.

Keywords: Atenolol, Cardiac toxicity, Isoproterenol, Propranolol, Tinospora cordifolia

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REFERENCES
1. Rona G. Catecholamine cardiotoxicity. J Mol Cell Cardiolo 1985; 17(4):291–306.
2. Jay LZ, Hassan MA. The role of oxidants and free radicals in reperfusion injury. Cardiovasc Res 2006; 70(2):181–90.
3. Thompson JA, Hess ML. The oxygen free radical system: a fundamental mechanism in the production of myocardial necrosis. Prog Cardiovasc Dis 1968; 28(6): 449–62.
4. Chishti H. The Traditional Healer’s handbook: A classic guide to the medicine of Avicenna. Bear and company ISBN 89281-438-1; 1991.
5. Shimada K, Nishikimi T, Kawarabayashi T, Takeuchi K, Takeda T. Effect of prolonged beta-adrenergic blockade induced by atenolol on left ventricular remodeling after acute myocardial infarction in the rat. Jpn Heart J 1995; 36:81–9.
6. Zhou R, Xu Q, Zheng P, Yan L, Zheng J, Dai G. Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. Eur J Pharmacol 2008; 586:244–50.
7. Pinelli A, Trivulzio S, Tomasoni L, Brenna S, Bonacina E, Accinni R. Isoproterenol-induced myocardial infarction in rabbits Protection by propranolol or labetalol: A proposed non-invasive procedure. Eur J Pharm Sci 2004; 23:277–85.
8. Cruickshank JM, Prichard BNC. Beta-blockers in clinical practice London: Churchill Livingstone. 1996.
9. Rao PR, Kumar VK, Viswanath RK, Subbaraju GV. Cardioprotective activity of alcoholic extract of Tinospora cordifolia in ischemia-reperfusion induced myocardial infarction in rats. Biol Pharm Bull 2005; 28(12):2319-22.
10. Xu LP, Shen FM, Shu H, Miao CY, Jiang YY, Su DF. Synergism of atenolol and amlodipine on lowering and stabilizing blood pressure in spontaneously hypertensive rats. Fund Clin Pharmacol 2004; 18(1):33-8.
11. Chakraborty M, Asdaq SM. Interaction of Semecarpus anacardium L. with propranolol against isoproterenol induced myocardial damage in rats. Indian J Exp Biol 2011; 49:200-6
12. Panda VS, Naik SR. Evaluation of cardioprotective activity of Ginkgo biloba and Ocimum sanctum in rodents. Altern Med Rev 2009; 14(2):161.
13. Sun M, Zigman S. An improved spectrophotometric assay for superoxide dismutase based on epinephrine auto-oxidation. Anal Biochem 1978; 90:81–89
14. Clairborne A. Catalase activity. In: Greenwald RA, editor. CRS handbook of methods in oxygen radical research. Boca Raton, FL: CRS Press; 1991, pp 283–284
15. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 70–77
16. Vennila L, Pugalendi KV. Protective effect of sesamol against myocardial infarction caused by isoproterenol in Wistar rats. Redox Rep 2010; 15:36–42.
17. Yogeeta SK, Gnanapragasam A, Kumar SS, Subhashini R, Sathivel A, Devaki T. Synergistic interactions of ferulic acid with ascorbic acid: its cardioprotective role during isoproterenol induced myocardial infarction in rats. Mol cel biochem 2006; 283(1-2):139-46.
18. Saravanan G, Ponmurugan P, Sathiyavathi M, Vadivukkarasi S, Sengottuvelu S. Cardioprotective activity of Amaranthus viridis Linn: effect on serum marker enzymes, cardiac troponin and antioxidant system in experimental myocardial infarcted rats. Int J Cardiol 2013; 165(3):494-98.
19. Sun F, Hamagawa E, Tsutsui C, Sakaguchi N, Kakuta Y, Tokumaru S, Kojo S. Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 2003; 65:101–07,
20. Halliwell B. How to characterize a biological antioxidant. Free Radic Res commun 1990; 9(1):1-32.
21. Priya LB, Baskaran R, Elangovan P, Dhivya V, Huang CY, Padma VV. Tinospora cordifolia extract attenuates cadmium-induced biochemical and histological alterations in the heart of male Wistar rats. Biomed Pharmacother 2017; 87:280-7.
22. Rajurkar NS, Hande SM. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian J Pharm Sci 2011; 73(2):146–51.
23. López-Sendón J, Swedberg K, McMurray J, Tamargo J, Maggioni AP, Dargie H. Expert consensus document on ?-adrenergic receptor blockers. Eur Heart J 2004; 25:1341–62.
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savant, C., V. KULKARNI, and P. HABBU. “PHARMACODYNAMIC INTERACTION OF TINOSPORA CORDIFOLIA WITH ATENOLOL AND PROPRANOLOL IN ISOPROTERENOL INDUCED CARDIAC NECROSIS IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 5, Mar. 2021, doi:10.22159/ijpps.2021v13i5.40218.
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