IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF CARDIOSPERMUM HALICACABUM L. AGAINST EAC CELL LINE
Keywords:Cardiospermum halicacabum L, Ehrlich Ascites Carcinoma, Free radicals, Antioxidant
Objective: To investigate the in vitro antioxidant and anticancer activity of chloroform and ethanol extracts of Cardiospermum halicacabum L. leaves.
Methods: Phytochemicals were analysed by using standard methods. In vitro antioxidant studies were carried out for the chloroform and ethanol extracts of the Cardiospermum halicacabum using various free radical models such a DPPH, Reducing power assay, nitric oxide scavenging, hydrogen peroxide (H2O2) scavenging, super oxide scavenging activity and ABTS. In vitro cytotoxic assay such as trypan blue dye exclusion and MTT assays were carried out against EAC cell line.
Results: Preliminary phytochemical analysis of the Cardiospermum halicacabum L. was carried out and it revealed the presence of alkaloids, coumarine, flavones, saponins, steroids, sugar, tannins and terpenoids. The results revealed that the chloroform extract has significant antioxidant potential than ethanol extract. The result revealed that the chloroform extracts of Cardiospermum halicacabum L. showed pronounced anticancer activity against Ehrlich Ascites Carcinoma (EAC) cell line than ethanol extract.
Conclusion: The result of the present study concluded that the chloroform extract of Cardiospermum halicacabum L have significant antioxidant and anticancer activity then the ethanolic extract. The potential antioxidant and anticancer activity of Cardiospermum halicacabum L might be due to the presence of phytochemicals.
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