• Kirthika P. Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Dheeba B. Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Sivakumar R. Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Sheik Abdulla S. Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.


Nanoparticles, Terminalia chebula, Zeta potential, AgNPs, TEM, XRD


Objective: The study was aimed to synthesis and characterization of silver nanoparticles from five different herbal plants (Terminalia chebula, Mimusops elengi, Myristica fragrans, Centella asiatica and Hemidesmus indicus).

Methods: The qualitative analysis of plant extracts was performed to determine the presence of secondary metabolites. The plant mediated silver nanoparticles were synthesized. The color changed into brown to black color indicating the formation of AgNPs. The characterization of synthesized AgNPs was carried out by different methods such as UV-Vis Spectra, FE-TEM, Particle size analysis, Zeta potential analysis, XRD and FTIR. The antimicrobial activity of synthesized silver nanoparticles also examined against three fungi and bacteria.

Results: The UV wave length of AgNPs is from 300 to 450 nm. The average size of AgNPs 581 d.nm, zeta potential is -13.3 mV. The FTIR results show that AgNPs contains the functional groups. In antimicrobial activity of all AgNPs synthesized by five plants inhibits the growth of bacteria and Terminalia chebula showed maximum effect. The XRD pattern clearly confirmed that the synthesized silver nanoparticles are crystalline in nature. TEM results shows that synthesized silver nanoparticles are round in shape.

Conclusion: The green synthesis of nanoparticles shows that cost-effective, environmentally friendly, and safe for human therapeutic use. Color change, UV-Vis spectra, TEM and XRD analysis confirmed the stability of synthesized AgNPs.



Download data is not yet available.


Huang J, Lin L, Li Q, Sun D, Wang Y, Lu Y, et al. Continuousflow biosynthesis of silver nanoparticles by lixivium of sundried Cinnamomum camphora leaf in tubular microreactors. J Ind Eng Chem Res 2008;47:6081-90.

Ahmad N, Sharma S, Alam M K, Singh V N, Shamsi, S F, Mehta, B R, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. J Colloids Surf 2010;81:81-6.

Elechiguerra JL, Burt JL, Morones JR, Bragado AC, Gao X, Lara HH and Yacaman M J. Interaction of silver nanoparticles with HIV-1. J Nanobiotech 2005;3:6.

Arango A C, Johnson LR, Bliznyuk VN, Schlesinger Z, Carter SA, Horhold HH. Efficient titanium oxide/conjugated polymer photovoltaics for solar energy conversion. J Adv Mater 2000;12:1689-92.

Tsujino K, Matsumura M. Morphology of nanoholes formed in silicon by wet etching in solutions containing HF and H2O2 at different concentrations using silver nanoparticles as catalysts. Electrochim. J Acta 2007;53:28-34.

Jun Y W, Huh YM, Choi JS. Nanoscale size effect of magnetic nanocrystals and their utilization for cancer diagnosis via magnetic resonance imaging. J Am Chem Soc 2005;127:5732-33.

Bao J, Chen W, Liu T, Zhu Y, Jin P, Wang L, et al. Bifunctional Au-Fe3O4 nanoparticles for protein separation. J ACS Nano 2007;1:293-8.

Bharat Reddy D, Reddy TCM, Jyotsna G, Satish Sharan, Nalini Priya, Lakshmipathi V, Pallu Reddanna. Chebulagic acid a COX–LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz induces apoptosis in COLO-205 cell line. J of Ethnopharmacology 2009;124:506-12.

Mazzola L. Commercializing nanotechnology. J Nature Biotechnology 2003;21:1137-43.

Willner I, Baron R, Willner B. Growing metal nanoparticles by enzymes. J Adv Mater 2006;18:1109-20.

Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paraliker KM and Balasubramanya RH. Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. J Mater Lett 2007;61:1413-8.

Shankar SS, Ahmed A, Akkamwar B, Sastry M, Rai A, Singh A. Biological synthesis of triangular gold nanoprism. J Nature 2004;3:482.

Kumar V and Yadav SK. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 2009;84:151-7.

Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB and Ramírez JT. The bactericidal effect of silver nanoparticles. J Nanotechnology 2005;16:2346-53.

Rajkumara S, Pandiselvi A and Sandhiya G. Isolation of Chemical Constituents from Mimusops elengi bark and Evaluation of Antiâ€inflammatory activity. Int J Phytopharmacy 2012;3(1):9â€15.

Jebakumar.T Immanuel Edison, Sethuraman.M,G. Instant green synthesis of silver nanoparticles using Terminalia chebula fruit extract and evaluation of their catalytic activity on reduction of methylene blue. J Process Biochemistry 2012;47:1351-7.

Mulvaney P. Nanoparticles and applications. J Langmuir 1996;12:788.

Prabhu N, Raj Divya T, Gowri Yamuna K, Ayisha siddiqua S, Joseph pushpa D. Dig J Nanomet Bios 2010;5(1):185.

Daizy Philip. Green synthesis of gold an dsilvernano particles using Hibiscus rosasinensis. J Physica 2009;E 42 (2010):1417-24.

Neveen Abdel-Raouf, Ibraheem B M, Ibraheem, and Nouf Mohammad Al-Enazi. Green biosynthesis of gold nanoparticles using Galaxaura elongata and characterization of their antibacterial activity. Arabian J of Chemistry 2013;1878-535.

Bankar AV, Kumar AR, Zinjarde SS. Removal of chromium (VI) ions from aqueous solution by adsorption onto two marine isolates of Yarrowia lipolytica. J Hazard Mater 2009;170:487-94.

Sathyavathi R, Krishna MB, Rao SV, Saritha R and Rao DN. Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. J Adv Sci Lett 2010;3:138-43.

SuryaPrakash DV, Sree Satya N, Sumanjali Avanigadda and Meena Vangalapati. Pharmacological Review on Terminalia Chebula. Int J of Res in Pharm and Biol Sci 2012;3:2229-3701.

Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K and Thangamani S. Asian Pac J Trop Biomed 2012;2(7):574-80.

Prabhu N, Divya T.R, Yamuna G. Synthesis of silver phyto nanoparticles and their antibacterial efficacy. Digest J Nanomater Biostruct 2010;5:185-9.

Williams D. Synthesis of silver phyto nanoparticles. J Biomaterials 2008;29:1737.

Kim TG, Kang SY, Jung KK, Kang JH, Lee E, Han HM and Kim SH. Antiviral activities of extracts isolated from Terminalis chebula Retz., Sanguisorba officinalis L., Rubus coreanus Miq and Rheum palmatum L. against hepatitis B virus. J Phytotherapy Res 2001;15(8):718-20.

Saifuddin N, Wong CW and Nur Yasumira AA. Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. E J Chem 2009;6(1):61-70.

Ankanna S, Prasad TNVKV, Elumalai EK and S avithramma N. Production of biogenic silver nanoparticles using Boswellia ovalifoliolata stem bark. Dig J Nanomater Biostruct 2010;5(2):369-72.

Dubey M, Bhadauria S, Kushwah BS. Green synthesis of nanosilver particles from extract of Eucalyptus hybrida (Safeda) leaf. Dig J Nanomat Biostruct 2009;4(3):537-43



How to Cite

P., K., D. B., S. R., and S. A. S. “PLANT MEDIATED SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 304-10,



Original Article(s)