DOES LOW DOSE OF PIPER NIGRUM EXTRACT EXERT ANTI-OBESITY ACTIVITY?

  • IRAM NAZISH Department of Pharmaceutical Sciences, CMR College of Pharmacy, Hyderabad, Telangana, India http://orcid.org/0000-0002-1038-3557
  • FAIZA SHAIKH Department of Pharmacology, H K College of Pharmacy, Oshiwara, Mumbai, India
  • GAURANG SAWANT Department of Pharmacology, H K College of Pharmacy, Oshiwara, Mumbai, India

Abstract

Objective: To evaluate anti-obesity effect of aqueous P. nigrum extract in a murine model of high-fat diet (HFD) induced obesity.


Methods: Male Wistar rats were fed with a high-fat diet (HFD) (20g/day/rat) for a period of 50 d to induce obesity. Aqueous P. nigrum extract (20 mg/kg) administered orally to high-fat diet (HFD) fed rats from 8th day to 50th day (total 42 d). The parameters like gain in body weight, serum lipids, insulin and leptin were measured.


Results: The rats treated with extract showed a significant reduction in body weight gain, serum insulin, leptin, lipids as compared to rats fed with only a high-fat diet (HFD). In addition, the extract-treated group showed a considerable rise in high-density lipoprotein (HDL-C) level (29.61±7.68 mg/dl) as compared to the control group (23.23±9.69 mg/dl).


Conclusion: The results indicate that aqueous P. nigrum extract possess the potential to reduce obesity markers in a high-fat diet (HFD) fed rats.

Keywords: Piper nigrum, High fat diet, Obesity, Insulin, Lipid

Downloads

Download data is not yet available.

Author Biographies

IRAM NAZISH, Department of Pharmaceutical Sciences, CMR College of Pharmacy, Hyderabad, Telangana, India

ASSOCIATE PROFESSOR

 

PHARMACOLOGY

FAIZA SHAIKH, Department of Pharmacology, H K College of Pharmacy, Oshiwara, Mumbai, India

Assistant Professor

References

1. Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet 2014;384:766-81.
2. Aune D, Sen A, Prasad M, Norat T, Janszky I, Tonstad S, et al. BMI and all-cause mortality: a systematic review and non-linear dose-response meta-analysis of 230 cohort studies with 3.74 million deaths among 30.3 million participants. Br Med J 2016;4:353.
3. Patel SE, Vyas NI. Validated spectrofluorimetric method for estimation of piperine in an ayurvedic formulation. Asian J Pharm Clin Res 2012;5:231-3.
4. Kshirsagar AD, Mohite R, Aggrawal AS, Suralkar UR. Hepatoprotective medicinal plants of Ayurveda-a review. Asian J Pharm Clin Res 2011;4:1-8.
5. Hasani Ranjbar S, Jouyandeh Z, Abdollahi M. A systematic review of anti-obesity medicinal plants-an update. Diabetes Metab Syndr 2013;12:28.
6. Yun JW. Possible anti-obesity therapeutics from nature–a review. Phytochemistry 2010;71:1625-41.
7. Kolhe SR, Borole P, Patel U. Extraction and evaluation of piperine from Piper nigrum Linn. Int J Appl Biol Pharm 2011;2:144-9.
8. Ahmad N, Fazal H, Abbasi BH, Rashid M, Mahmood T, Fatima N. Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L. Plant Cell Tissue Organ Cult 2010;102:129-34.
9. Kale MA, Bindu SM, Khadkikar P. Role of antioxidants and nutrition in oxidative stress: a review. Int J Appl Pharm Sci 2015;7:1-4.
10. Meriga B, Parim B, Chunduri VR, Naik RR, Nemani H, Suresh P, et al. Antiobesity potential of piperonal: promising modulation of body composition, lipid profiles and obesogenic marker expression in HFD-induced obese rats. Nutr Metab 2017;14:72.
11. Trease GE, Evans WC. Pharmacognosy: 7th Edition. Philadelphia: Bailliere Tindal Publisher and distributer; 1989.
12. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. J Am Association Clin Chem 1972;18:499-502.
13. Shah SS, Shah GB, Singh SD, Gohil PV, Chauhan K, Shah KA, et al. Effect of piperine in the regulation of obesity-induced dyslipidemia in high-fat diet rats. Indian J Pharmacol 2011;43:296.
14. Shah SS, Shah GB, Singh SD, Gohil PV, Chauhan K, Shah KA, et al. Effect of piperine in the regulation of obesity-induced dyslipidemia in high-fat diet rats. Indian J Pharmacol 2011;43:296.
15. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutr Res Rev 2010;23:270-99.
16. Parim B, Harishankar N, Balaji M, Pothana S, Sajjalaguddam RR. Effects of Piper nigrum extracts: restorative perspectives of high-fat diet-induced changes on lipid profile, body composition, and hormones in sprague–dawley rats. Pharm Biol 2015;53:1318-28.
17. Bhandari U, Kumar V, Khanna N, Panda BP. The effect of high-fat diet-induced obesity on cardiovascular toxicity in Wistar albino rats. Hum Exp Toxicol 2011;30:1313-21.
18. Harbilas D, Brault A, Vallerand D, Martineau LC, Saleem A, Arnason JT, et al. Populus balsamifera L. (Salicaceae) mitigates the development of obesity and improves insulin sensitivity in a diet-induced obese mouse model. J Ethnopharmacol 2012;141:1012-20.
19. Christodoulides C, Vidal Puig A. PPARs and adipocyte function. Mol Cell Endocrinol 2010;318:61-8.
20. Li X, Choi Y, Yanakawa Y, Park T. Piperonal prevents high-fat diet-induced hepatic steatosis and insulin resistance in mice via activation of adiponectin/AMPK pathway. IJO 2014;38:140-7.
21. Shukla R, Kashaw V. Development, characterization and evaluation of herbal ointment containing momordica charantia, pongamia glabra and piper nigrum. J Drug Delivery Ther 2019;9:136-40.
22. Kuraji R, Fujita M, Ito H, Hashimoto S, Numabe Y. Effects of experimental periodontitis on the metabolic system in rats with diet-induced obesity (DIO): an analysis of serum biochemical parameters. Odontology 2018;106:162-70.
Statistics
40 Views | 42 Downloads
Citatons
How to Cite
NAZISH, I., F. SHAIKH, and G. SAWANT. “DOES LOW DOSE OF PIPER NIGRUM EXTRACT EXERT ANTI-OBESITY ACTIVITY?”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 5, Mar. 2020, pp. 41-46, doi:10.22159/ijpps.2020v12i5.27942.
Section
Original Article(s)