IN VITRO ANTIMICROBIAL ACTIVITY AND MIC OF THE EXTRACELLULAR ETHYL ACETATE CRUDE EXTRACT OF ENDOPHYTIC FUNGI FUSARIUM SP. ISOLATED FROM TEPHROSIA PURPUREA ROOT
DOI:
https://doi.org/10.22159/ijpps.2019v11i3.28230Keywords:
Endophytes, Antimicrobial, MIC, Tephrosia purpurea root, Fusarium spAbstract
Objective: The objective of the present investigation was to find antimicrobial and MIC of endophytic fungi Fusarium sp. isolated from Tephrosia purpurea root.
Methods: Well diffusion assay was performed to find out the antimicrobial activity and Resazurin dye reduction method was performed to find out MIC of the extract.
Result: The extract showed the highest zone of inhibition of 22.66±0.57 mm, (Bacillus subtilis, MTCC-441) for Gram-positive bacteria and 20.66±0.57 mm, (E. coli, MTCC-443) for Gram-negative bacteria. Furthermore, the MIC of the extract was found to be (31.25 µg/ml-125 µg/ml).
Conclusion: Hence, the endophytic fungi isolated from the Tephrosia purpurea root, i.e. Fusarium sp. showed good antimicrobial activity and hence can be used to find a novel drug.
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