IN VITRO ANTIMICROBIAL ACTIVITY AND MIC OF THE EXTRACELLULAR ETHYL ACETATE CRUDE EXTRACT OF ENDOPHYTIC FUNGI FUSARIUM SP. ISOLATED FROM TEPHROSIA PURPUREA ROOT

  • DEEPENDRA THAKUR Department of Microbiology, Banglore University, Banglore, India,
  • KAMANA SAHANI Department of Microbiology, Andhra University, Visakhapatnam, India

Abstract

Objective: The objective of the present investigation was to find antimicrobial and MIC of endophytic fungi Fusarium sp. isolated from Tephrosia purpurea root.


Methods: Well diffusion assay was performed to find out the antimicrobial activity and Resazurin dye reduction method was performed to find out MIC of the extract.


Result: The extract showed the highest zone of inhibition of 22.66±0.57 mm, (Bacillus subtilis, MTCC-441) for Gram-positive bacteria and 20.66±0.57 mm, (E. coli, MTCC-443) for Gram-negative bacteria. Furthermore, the MIC of the extract was found to be (31.25 µg/ml-125 µg/ml).


Conclusion: Hence, the endophytic fungi isolated from the Tephrosia purpurea root, i.e. Fusarium sp. showed good antimicrobial activity and hence can be used to find a novel drug.

Keywords: Endophytes, Antimicrobial, MIC, Tephrosia purpurea root, Fusarium sp

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References

1. Guo B, Wang Y, Sun X, Tang K. Bioactive natural products from endophytes: a review. Appl Biochem Microbiol 2008;44:136-42.
2. Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003;67:491-502.
3. Berdy J. Bioactive microbial metabolites. A personal view. J Antibiot 2005;58:1-26.
4. Gunatilaka AA. Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity and implications of their occurrence. J Nat Products 2006;69:509-26.
5. Kusari S, Zuhlke S, Spiteller M. Effect of artificial reconstitution of the interaction between the plant Camptotheca acuminata and the fungal endophyte Fusarium solani on camptothecin biosynthesis. J Nat Prod 2011;74:764-75.
6. Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, et al. Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiol Res 2010;165:437-49.
7. Strobel GA. Microbial gifts from rain forests. Can J Plant Pathol 2002a;24:14-20.
8. Strobel GA. Rainforest endophytes and bioactive products. Crit Rev Biotechnol 2002b;22:315-33.
9. Wiyakrutta S, Sriubolmas N, Panphut W, Thongon N, Danwiserkanjana K, Ruangrungsi N, et al. Endophytic fungi with antimicrobial, anti-cancer, anti-malarial activities isolated from Thai medicinal plants. World J Microbiol Biotechnol 2004;20:265-72.
10. Huang WY, Cai YZ, Xing J, Corke H, Sun M. Potential antioxidant resource: endophytic fungi isolated from traditional Chinese medicinal plants. Econ Bot 2007;61:14-30.
11. Kharwar RN, Gond SK, Kumar A, Kumar J, Mishra A. A comparative study of endophytic and epiphytic fungal association with the leaf of Eucalyptus citriodora Hook and their antimicrobial activity. World J Microbiol Biotechnol 2010;26:1941-8.
12. Fisher PJ, Petrini O. Tissue specificity by fungi endophytic in Ulex europaeus. Sydow 1987;40:46-50.
13. Suryanarayanan TS, Kumaresan V, Johnson JA. Foliar fungal endophytes from two species of the mangrove Rhizophora. Can J Microbiol 1998;44:1003-6.
14. Schulz B, Wanke U, Drager S, Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol Res 1993;97:1447-50.
15. Clinical Laboratory Standards Institute (CLSI), Performance standards for antimicrobial disk susceptibility tests, approved standard, M2-A8. 8th ed. CLSI, Wayne, PA, U. S. A; 2005.
16. NCCLS, National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing. In: Ninth Informational Supplement, Wayne, Pennsylvania M 100-S9; 1999.
17. Zhang Y, Mu J, Feng Y, Kang Y, Zhang J, Juan P, et al. Broad-spectrum antimicrobial epiphytic and endophytic fungi from marine organisms: isolation, bioassay, and taxonomy. Mar Drugs 2009;7:97-112.
18. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol 2011;27:3005-8.
19. Clinical and Laboratory Standards Institute (CLSI), Performance standards for antimicrobial disk susceptibility tests; approved standard. 10th Ed. M02-A10. CLSI, Wayne, PA. U. S. A; 2009.
20. Clinical and Laboratory Standards Institute (CLSI), Method for antifungal disk diffusion susceptibility testing of yeasts. Second edition. M44-A2. 2nd Ed. Wayne, PA, U. S. A; 2009.
21. Clinical Laboratory Standards Institute (CLSI), Method for antifungal disk diffusion susceptibility testing of filamentous fungi; proposed guideline. Document M51-P. CLSI, Wayne, PA, U. S. A; 2009.
22. Arivudainambi USE, Anand TD, Shanmugaiah V, Karunakaran C, Rajendran A. Novel bioactive metabolites producing endophytic fungus Colletotrichum gloeosporioides against multidrug-resistant Staphylococcus aureus. FEMS Immunol Medical Microbiol 2011;61:340-5.
23. Clinical Laboratory Standards Institute (CLSI), Development of in vitro susceptibility testing criteria and quality control parameters. 3rd ed. M23-A3. CLSI, Wayne, PA. U. S. A.; 2008.
24. Clinical Laboratory Standards Institute (CLSI), Performance standards for antimicrobial susceptibility testing: 21st informational supplement. M100-S21. CLSI, Wayne, PA, U. S. A.; 2011.
25. Gulnaz AR, Savitha G. Evaluation of antimicrobial activity of leaf and stem extracts of sidda medicinal plant Sida cordata. Int J Med Pharma Sci 2013;3:39-50.
26. Martin A, Camacho M, Portaels F, Carlos Palomino C. Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method. Antimicrob Agents Chemother 2003;47:3616-9.
27. Zou WX, Meng JC, Lu H, Chen GX, Shi GX, Zhang TY, et al. Metabolites of Colletotrichum gloeosporioides, an endophytic fungus in Artemisia mongolica. J Nat Prod 2000;63:1529-30.
28. Shu RG, Wang FW, Yang YM, Liu YX, Tan RX. Antibacterial and xanthine oxidase inhibitory cerebrosides from Fusarium sp., IFB-121 an endophytic fungus in Quercus variabillis. Lipids 2004;39:667-73.
29. Kamana Sahani, Deependra Thakur, KPJ Hemalatha, Arkajit Ganguly. Antibacterial activity of endophytes from selected medicinal plants. Int J Adv Res 2017;5:2076-86.
30. Chareprasert S, Piapukiew J, Thienhirun S, Anthony J, Whalley S, Sihanonth P. Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman merr. World J Microbiol Biotechnol 2006;22:481-6.
31. Wu JG, Peng W, Zeng PY, Wu YB, Yi J, Wu JZ. Antimicrobial activity and cytotoxicity of endophytes from Scapania verrucosa heeg. Genetics Mol Res 2013;12:916-24.
32. Raviraja NS, Maria GL, Sridhar KR. Antimicrobial evaluation of endophytic fungi inhabiting medicinal plants of the Western Ghats of India. Engin Life Sci 2006;6:515-20.
33. Wang FW, Jiao RH, Cheng AB, Tan SH, Song YC. Antimicrobial potentials of endophytic fungi in Quercus variabillis, brefeldin a obtained from Cladosporium sp. World J Microbiol Biotechnol 2007:23:79-83.
34. Xu L, Zhou L, Zhao J, Li J, Li X, Wang J. Fungal endophytes from Dioscorea zingiberensis rhizomes and their antibacterial activity. Lett Appl Microbiol 2007;46:68-72.
35. Verma S, Singh SP. Current and future status of herbal medicines. Veterin World 2008;1:347-50.
36. Ramos HP, Braun GH, Pupo MT, Said S. Antimicrobial activity of endophytic fungi Arthinium state of Apiospora montagnei Sacc. and Papulospora immerse. Brazilian Arch Biol Technol 2010;53:629-32.
37. Refaei J, Jones EBG, Sakayaroj J, Santhanam J. Endophytic fungi from Rafflesia cantleyi: species diversity and antimicrobial activity. Mycosph 2011;2:429-47.
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How to Cite
THAKUR, D., and K. SAHANI. “IN VITRO ANTIMICROBIAL ACTIVITY AND MIC OF THE EXTRACELLULAR ETHYL ACETATE CRUDE EXTRACT OF ENDOPHYTIC FUNGI FUSARIUM SP. ISOLATED FROM TEPHROSIA PURPUREA ROOT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 3, Jan. 2019, pp. 48-53, doi:10.22159/ijpps.2019v11i3.28230.
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