GCMS ANALYSIS OF ENDOPHYTIC FUNGI CURVULARIA AERIA MTCC-12847 ISOLATED FROM TRIBULUS TERRESTRIS L.

Authors

  • Kamana Sahani Department of Microbiology, Andhra University, Visakhapatnam, India
  • DEEPENDRA THAKUR Department of Microbiology, Banglore University, Banglore, India

DOI:

https://doi.org/10.22159/ijpps.2019v11i4.28440

Keywords:

Endophytic fungi, Tribulus terrestris L, GCMS analysis

Abstract

Objective: The objective of the present investigation was to perform the Gas Chromatography-mass spectrometry (GCMS) analysis of endophytic fungi Curvularia aeria MTCC-12847 isolated from Tribulus terrestris L. to find out the active compound present in the extract.

Methods: The endophytic fungi were isolated from the plant Tribulus Terrestris L., leaf which was cultivated in optimized media for the production of secondary metabolites and was extracted using ethyl acetate. Ethyl acetate extract was used for the Gas Chromatography-mass spectrometry (GCMS) analysis.

Results: GC-MS analysis of ethyl acetate extract of endophytic fungi revealed the presence of various secondary metabolites, the highest amount present was Palmitic acid (24.54%) and Lowest was Dimethyl 1-phenyl-7-methyl-1-hydroxy-1,4-dihydronaphthalene-2,3-dicarboxylate (5.76%).

Conclusion: The endophytic fungal Curvularia aeria MTCC-12847 extract isolated from the Tribulus terrestris L. shows the presence of various bioactive compounds.

Downloads

Download data is not yet available.

References

Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 13th edn. Pune: Nirali Prakashan Publisher; 2007. p. 370.

Antonio J, Uelmen J, Rodriguez R, Earnes C. The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. Int J Sport Nutr Exerc Metab 2000;10:208-15.

Cai L, Wu Y, Zhang J, Pei F, Xu Y, Xie S. Steroidal saponins from Tribulus terrestris. Planta Med 2001;67:196-8.

Mishra NK, Biswal G, Chowdary KA, Mishra G. Anti-arthritic activity of Tribulus terrestris studied in freund’s-adjuvant induced arthritic rats. J Adv Pharm Technol Res 2013;4:41-8.

Baburao B, Rajyalakshmi G, Venkatesham A, Kiran G, Shyamsunder A, Gangarao B. Anti-inflammatory and antimicrobial activities of methanolic extract of Tribulus terrestris L. plant. Int J Chem Sci 2009;7:1867-72.

Oh JS, Baik SH, Ahn EK, Jeong W, Hong SS. Anti-inflammatory activity of Tribulus terrestris in RAW264.7 cells. J Immunol 2012;88:54-2.

Oh HK, Park SJ, Moon HD, Jun SH, Choi NY, You YO. Tribulus terrestris inhibits caries-inducing properties of Streptococcus mutants. J Med Plants Res 2011;5:6061-66.

Zhang S, Li H, Yang SJ. Tribulosin protects rat hearts from ischemia/reperfusion injury. Acta Pharmacol Sin 2010;31:671-8.

Ivanova A, Serly J, Dinchev D, Ocsovszki I, Kostova I, Molnar J. Screening of some saponins and phenolic components of Tribulus terrestris and Smilax excelsa as MDR modulators. In Vivo 2009;23:545-50.

Svetla A, Zlatina G, Maria K, Georgi A, Valentin L, Tsanko M. Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells. J Biosci Biotech 2013;2:25-32.

Neychev VK, Nicolova E, Zhelev N, Mitev VI. Saponins from Tribulus terrestris L. are less toxic for normal human fibroblasts than for many cancer lines: Influence on apoptosis and proliferation. Exp Med Biol 2007;232:126-33.

Kumar M, Soni AK, Shukla S, Kumar A. Chemopreventive potential of Tribulus terrestris against 7, 12-dimethylbenz (a) anthracene induced skin papillomagenesis in mice. Asian Pac J Cancer Prev 2006;7:289-94.

Bacon CW, White JF. Microbial endophytes. Marcel Dekker Inc, New York; 2000.

Petrini O, Fokkema NJ, Van den Heuvel J. Microbiology of the Phyllosphere. Cambridge University Press, Cambridge; 1986.

Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW. Bacterial endophytes in agricultural crops. Can J Microbiol 1997;43:895-914.

Verma VC, Gond SK, Kumar A, Mishra A, Kharwar RN, Gange AC. Endophytic actinomycetes from Azadirachta indica A. Juss.: isolation, diversity, and anti-microbial activity. Microb Ecol 2009;57:749-75.

Saikkonen K, Wali P, Helander M, Faeth SH. Evolution of endophyte-plant symbiosis. Trends Plant Sci 2004;9:275-80.

Schulz B, Boyle C, Draeger S, Rommert A, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004.

Strobel GA, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003;67:491-502.

Fisher PJ, Petrini O. Tissue specificity by fungi endophytic in Ulex europaeus. Sydow 1987;40:46-50.

Suryanarayanan TS, Kumaresan V, Johnson JA. Foliar fungal endophytes from two species of the mangrove Rhizophora. Can J Microbiol 1998;44:1003-6.

Schulz B, Wanke U, Drager S, Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol Res 1993;97:1447-50.

Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol 2011;27:3005-8.

Mohammed A, Imad H, Autosomal STR. From locus information to next-generation sequencing technology. Res J Biotechnol 2013;8:92-105.

Kareem MA, Hussein AO, Hameed IH. Y-chromosome short tandem repeat, typing technology, locus information, and allele frequency in the different population. Afr J Biotechnol 2015;14:2175-8.

Imad H, Mohammed A, Aamera J. Genetic variation and DNA markers in forensic analysis. Afr J Biotechnol 2014a;13:3122-36.

Imad H, Mohammed A, Cheah Y, Aamera J. Genetic variation of twenty autosomal STR loci and evaluate the importance of these loci for forensic genetic purposes. Afr J Biotechnol 2014b;13:1-9.

Imad H, Muhanned A, Aamera J, Cheah Y. Analysis of eleven Y-chromosomal STR markers in middle and south of Iraq. Afr J Biotechnol 2014c;13:3860-71.

Hamza LF, Sabreen AK, Imad HH. Determination of metabolites products by Penicillium expansum and evaluating antimicrobial activity. J Pharmacogn Phytother 2015;7:194-220.

Published

01-04-2019

How to Cite

Sahani, K., and D. THAKUR. “GCMS ANALYSIS OF ENDOPHYTIC FUNGI CURVULARIA AERIA MTCC-12847 ISOLATED FROM TRIBULUS TERRESTRIS L”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 11, no. 4, Apr. 2019, pp. 26-32, doi:10.22159/ijpps.2019v11i4.28440.

Issue

Section

Original Article(s)