• Affo Dermane Department of Pharmacy, Universite de Lome, Togo,
  • Kafui Kpegba Laboratory of Organic Chemistry and Natural Substances, Universite de Lome, Togo
  • Kossi Metowogo Laboratory of Physiology-Pharmacology, Universite de Lomé, Togo
  • M. Kossi Joppa Laboratory of Physiology-Pharmacology, Universite de Lomé, Togo
  • A. Kodjo Aklikokou Laboratory of Physiology-Pharmacology, Universite de Lomé, Togo


Objective: This study aims to evaluate the fractions from whole extracts of roots and stem barks of Newbouldia laevis and their effect on sickling.

Methods: Hydroethanolic extracts of stem barks and roots of Newbouldia laevis were fractionated by the technics of cold precipitation in ethanol. The fractions obtained after phytochemical screening were subjected to Emmel test to evaluate their anti-sickling activity. Active fractions were tested for DPPH and AAPH assay (AAPH induced membrane lipoperoxidation and evaluation of reduction of hemolysis).

Results: Two fractions were obtained from each whole extract: supernatant and pellet fractions. Supernatants fractions obtained from whole roots barks extract and stem barks extract at a concentration of 30 mg/ml reduced sickling up respectively to 7% and 10% against 86% for the control. Pellets fractions obtained from the both extracts induced coagulation of SS blood at 30 mg/ml against 86% for the control.

Conclusion: Supernatants fractions of hydroethanolic whole extract of roots and stem backs of Newbouldia laevis promise as the potential source of active molecules against sickle cell disease.

Keywords: Sickling, supernatant fraction, Newbouldia laevis, sickle cell disease, SS genotype


Download data is not yet available.


1. Aygun B, Odame I. A global perspective on sickle cell disease. Pediatr Blood Cancer 2012;59:386-90.
2. Stuart MJ, Nagel RL. Sickle-cell disease. Lancet 2004;364:1343-60.
3. Bardakdjian J, Wajcman H. Epidemiologie de la drepanocytose. Rev Prat 2004;54:1531-3.
4. Montalembert M, Tshilolo L. Les progrès thérapeutiques dans la prise en charge de la drepanocytose sont-ils applicables en afrique subsaharienne? Med Trop 2007;67:612-6.
5. Penkert RR, Hurwitz JL, Thomas P, Rosch J, Dowdy J, Sun Y, et al. J. S. Inflammatory molecule reduction with hydroxyurea in children with sickle cell anemia. Haematologica 2018;103:50-4.
6. Rees DC, Robinson S, Howard J. How I manage cell transfusions in patients with sickle cell disease. Br J Haematol 2018;180:607-17.
7. Archana D, Dixitha M, Santhy KS. Antioxidant and anticlastogenic potential of Piper longum L. Int J Appl Pharm 2015;7:11-4.
8. Joppa KM, Vovor A, Eklu Gadegbeku K, Agbonon A, Aklikokou K, Gbeassor M. Effet de Morinda lucida Benth (Rubiaceae) et de Newbouldia laevis P. Beauv (Bignoniaceae) sur la falciformation. Med Trop 2008;68:251-6.
9. Kadebe ZT, Metowogo K, Bakoma B, Lawson Evi SP, Eklu Gadegbeku K, Aklikokou K, et al. Antidiabetic activity of Plumeria alba linn (Apocynaceae) root extract and fractions in streptozotocin-induced diabetic rats. Trop J Pharm Res 2016;15:87-95.
10. Harbone JB. Phytochemical methods. Chapman and hall eds New York; 1973. p. 354.
11. Chabra SC, Uiso F. A survey of the medicinal plants of Eastern Tanzanian for alkaloids, flavonoids, saponins, and tannins. Fitoterapia 1990;1:307-16.
12. Missebukpo A, Metowogo K, Diallo A, Lawson Evi P, Eklu Gadegbeku K, Aklikokou KA, et al. Antioxidant effects of Ixora coccinea linn in a rat model of ovalbumin-induced asthma. Afr J Pharm Pharmacol 2013;7:2794-800.
13. Metowogo K, Sama MS, Lawson Evi P, Eklu Gadegbeku K, Aklikokou AK, Gbeassor M. Effet anti-ulcereux de l'extrait hydroalcoolique d’écorce de tronc et de racine de Ficus polita vahl (Moraceae). J Rech Sci Univ Lome 2014;16:13-22.
14. Dai F, Miao Q, Zhou B, Yang L, Liu ZL. Protective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cells. Life Sci 2005;78:2488-93.
15. Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’her. Int J Appl Pharm 2016;8:7-11.
16. Kambale JK, Ngbolua KN, Mpiana PT, Mudogo V, Tshibangu DST, Wumba DMR, et al. Evaluation in vitro de lactivite antifalcemiante et effet antioxydant des extraits d’Uapaca heudelotii baill. (Euphorbiaceae). Int J Biol Chem Sci 2013;7:523-34.
17. Mpiana PT, Mudogo V, Tshibangu DS, Kitwa EK, Kanangila AB, Lumbu JB, et al. Antisickling activity of anthocyanins from Bombax pentadrum, Ficus capensis and Ziziphus mucronata: photodegradation effect. J Ethnopharmacol 2008;120:413-8.
18. Rakam GK, Raja S. Standardization and phytochemical screening of Bougainvillea glabra. Int J Curr Pharm Res 2017;9:75-80.
19. Mpiana PT, Mudogo V, Kabangu YF, Tshibangu DST, Ngbolua KN, Atibu EK, et al. Antisickling activity and thermostability of anthocyanins extract from a Congolese plant, Hymenocardia acida Tul. (Hymenocardiaceae). Int J Pharmacol 2009a;5:65-70.
20. Sawadogo Stanislas, Sanou Sagazaga Drissa, Dabire Prosper, Belemtougri Gourounga Raymond, Sawadogo Laya, De Leiris Joel, et al. Activité antifalcémiante d’extraits de trois plantes médicinales du Burkina Faso: Jatropha curcas, Khaya senegalensis et Dichrostachys cinerea. Int J Biol Chem Sci 2017;11:2016-27.
21. Aoret Asseny R, Souza A, Kpahey F, Konate K. Dichrostachys cinerea wight et Arn (Mimosaceae) hydro-alcoholic extract action on the contractility of tracheal smooth muscle isolated from guinea-pig. BMC Complementary Altern Med 2011;11:1-8.
22. Chohan M, Naugton DP, Jones SL, Opara EI. An investigation of the relationship between the antiinflammatory activity, polyphenolic content, and antioxidant activities of cooked and in vitro digested culinary herbs. Oxid Med Cell Longev 2012;8:627-43.
23. Lemonne N, Lamarre Y, Romana M, Mukisi Mukaza M, Hardy Dessources MD, Tarer V, et al. Does increase red blood cell deformability raises the risk for osteonecrosis in sickle cell anemia? Blood 2013;121:3054-6.
24. Kahkonen MP, Hopia AI, Vourela HJ, Rauha J, Pihlaja K, Kujala TH, et al. Antioxidant activity plant extracts containing phenolic compounds. J Agric Food Chem 1999;47:3954-62.
25. Ogunlana OE, Ogunlana OO. In vitro assessment of antioxidant activity of Newbouldia laevis. J Med Plant Res 2008;2:176-9.
31 Views | 26 Downloads
How to Cite
Dermane, A., K. Kpegba, K. Metowogo, M. Joppa, and A. Aklikokou. “ANTISICKLING ACTIVITY EVALUATION OF FRACTIONS OBTAINED FROM WHOLE EXTRACTS OF NEWBOULDIA LAEVIS P. BEAUV (BIGNONIACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 2, Dec. 2018, pp. 42-46, doi:10.22159/ijpps.2019v11i2.30112.
Original Article(s)